MicroRNA‑758 inhibits tumorous behavior in tongue squamous cell carcinoma by directly targeting metadherin

Zhang, Yulan; Zhao, Fu-Jun

doi:10.3892/mmr.2019.9805

Cited by 5 publications

(5 citation statements)

References 35 publications

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“…Three human TScc cell lines, Scc-9, cAL-27 and Scc-15, as well as normal gingival epithelial cells (ATcc ® PCS-200-014™) were purchased from the American Type Culture Collection (ATCC). Previous studies (26,27) have used the normal gingival epithelial cells as a control for TScc cell lines. dulbecco's modified Eagle's medium (dMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin solution (all Invitrogen; Thermo Fisher Scientific, Inc.) was utilized for cell culture.…”

Section: Methodsmentioning

confidence: 99%

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Zou

et al. 2020

Int J Mol Med

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A long non-coding RNA (lncRNA) called prostate cancer-associated non-coding RNA 1 (PRNCR1) serves crucial roles in the aggressive phenotypes of colorectal cancer and non-small cell lung cancer. However, there is little research on the expression profile, clinical value and detailed functions of PRNCR1 in tongue squamous cell carcinoma (TScc). The aim of the present study was to determine PRNCR1 expression in TScc and to examine the involvement of PRNCR1 in TScc progression. The molecular mechanisms behind the oncogenic effects of PRNCR1 in TScc cells were also investigated. PRNCR1 was revealed to be upregulated in TScc tumors and cell lines. The high PRNCR1 expression showed a significant correlation with tumor size, clinical stage, lymph node metastasis, and shorter overall survival times among patients with TScc. A PRNCR1-knockdown reduced TScc cell proliferation, migration and invasion, and increased apoptosis in vitro. Additionally, the PRNCR1-knockdown slowed down in vivo tumor growth of TScc cells. With regards to the mechanism, PRNCR1 acted as a competing endogenous RNA on microRNA-944 (miR-944) in TScc cells, and the effects of the PRNCR1-knockdown were reversed by an miR-944-knockdown. HOXB5 was validated as a direct target gene of miR-944 in TScc cells, and HOXB5 expression was found to be positively regulated by PRNCR1. Furthermore, resumption of HOXB5 expression reversed the tumor-suppressive actions of miR-944 in TScc cells. In conclusion, PRNCR1 acts as an oncogenic lncRNA in TScc through the upregulation of HOXB5 by sponging miR-944, thereby indicating a potential therapeutic target in TScc.

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Section: Methodsmentioning

confidence: 99%

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Zou

et al. 2020

Int J Mol Med

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“…Feng CZ’s research [ 45 ] suggested the roles of miRNA-124 in the microglial response to oxidative stress. In vitro, functional experiments revealed that up-regulation of miR-758 inhibited cell proliferation, migration, and invasion [ 46 , 47 ]. Mi-RNAs suppressed the proliferation, manifested as down-regulation in ovarian cancer tissues and cell lines [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanism of Malignant Transformation of Balb/c-3T3 Cells Induced by Long-Term Exposure to 1800 MHz Radiofrequency Electromagnetic Radiation (RF-EMR)

Ding

Xiang

et al. 2022

Bioengineering

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Purpose: We aimed to investigate RF-EMR-induced cell malignant transformation. Methods: We divided Balb/c-3T3 cells into sham and expo groups. The expo groups were exposed to a 1800 MHz RF continuous wave for 40 and 60 days, for 4 h per day. The sham group was sham-exposed. Cells were harvested for a cell transformation assay, transplantation in severe combined immune deficient (SCID) mice, soft agar clone formation detection, and a transwell assay. The mRNA microarray assay was used to declare key genes and pathways. Results: The exposed Balb/c-3T3 cells showed a strong increase in cell proliferation and migration. Malignant transformation was observed in expo Balb/c-3T3 cells exposed for 40 days and 60 days, which was symbolized with visible foci and clone formation. Expo Balb/c-3T3 cells that were exposed for 40 days and 60 days produced visible tumors in the SCID mice. Lipid metabolism was the key biological process and pathway involved. The mevalonate (MVA) pathway was the key metabolic pathway. The interacted miRNAs could be further research targets to examine the molecular mechanism of the carcinogenic effects of long-term exposure. Conclusion: Exposure for 40 and 60 days to 1800 MHz RF-EMR induced malignant transformation in Balb/c-3T3 cells at the SAR of 8.0 W/kg. We declared that lipid metabolism was the pivotal biological process and pathway. The MVA pathway was the key metabolic pathway.

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“…miR-758-3p also inhibits the malignant progression of retinoblastoma by directly targeting PAX6( 37 ). In addition, miR-758-3p also inhibits lung cancer cells ( 38 , 39 ). Based on these findings, it was speculated that miR-758-3p is closely associated with inflammation.…”

Section: Discussionmentioning

confidence: 99%

Increased expression of cyclooxygenase‑2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA‑758‑3p expression

et al. 2021

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Cyclooxygenase-2 (COX-2) is a common factor in inflammation, and its specific regulatory mechanism has not been fully elucidated. The present study aimed to investigate COX-2 mRNA and protein expression levels in synovium tissues and synovial fluid from patients with knee osteoarthritis (KOA), and determine the molecular mechanism by which microRNA (miRNA/miR)-758 regulates KOA via COX-2. A total of 37 patients with KOA and 29 patients with acute knee trauma (control group) were enrolled in the present study. Reverse transcription-quantitative PCR analysis was performed to detect miR-758-3p and COX-2 mRNA expression, while western blotting and ELISA were performed to detect COX-2 protein expression in synovium and synovial fluid, respectively. The dual-luciferase reporter assay was performed to verify the interaction between miR-758-3p and the 3'-untraslated region (UTR) of COX-2 mRNA. Synovial cells were transfected with agomiR-758-3p, and the MTT assay was performed to assess cell proliferation. The results demonstrated that COX-2 expression was higher in patients with KOA than those with acute knee trauma. Conversely, miR-758-3p expression was lower in patients with KOA than those with acute knee trauma. Notably, miR-758-3p interacted with the 3'-UTR of COX-2 mRNA to regulate its expression. Overexpression of miR-758-3p inhibited the expression and release of COX-2, as well as the proliferation of human KOA synovial cells. Taken together, these results suggest that COX-2 expression is upregulated in synovium tissues and synovial fluid from patients with KOA, which is associated with downregulated miR-758-3p expression. In addition, miR-758-3p affects the proliferation of synovial cells and the expression of relevant proteins in these cells, thus promoting the occurrence and development of KOA.

show abstract

MicroRNA‑758 inhibits tumorous behavior in tongue squamous cell carcinoma by directly targeting metadherin

Cited by 5 publications

References 35 publications

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Long non‑coding RNA PRNCR1 exerts oncogenic effects in�tongue squamous cell carcinoma in�vitro and in�vivo by sponging microRNA‑944 and thereby increasing HOXB5�expression

Molecular Mechanism of Malignant Transformation of Balb/c-3T3 Cells Induced by Long-Term Exposure to 1800 MHz Radiofrequency Electromagnetic Radiation (RF-EMR)

Increased expression of cyclooxygenase‑2 in synovium tissues and synovial fluid from patients with knee osteoarthritis is associated with downregulated microRNA‑758‑3p expression

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