MicroRNA-7 Inhibits Epithelial-to-Mesenchymal Transition and Metastasis of Breast Cancer Cells via Targeting FAK Expression

Kong, Xiangjun; Li, Gaopeng; Yuan, Yan; He, Yan; Wu, Xiaoli; Zhang, Weijie; Wu, Zhengsheng; Chen, Tingting; Wu, Wenyong; Lobie, Peter E.; Zhu, Tao

doi:10.1371/journal.pone.0041523

Cited by 169 publications

(148 citation statements)

References 50 publications

Supporting

Mentioning

142

Contrasting

Order By: Relevance

“…Similarly, miR-7 was reported to be downregulated in various cancers, including pancreatic and breast cancer, and was closely associated with the metastatic status of the patients (12,29). In the present study, we observed that the frequency of miR-7 promoter mutation was higher in the ADC type of lung cancer, although the difference was not statistically significant.…”

Section: Promoter Mutation Is Associated With Repressed Expression Ofcontrasting

confidence: 48%

See 1 more Smart Citation

Promoter mutation of tumor suppressor microRNA-7 is associated with poor prognosis of lung cancer

Zhao

Wang

Liao

et al. 2015

Molecular and Clinical Oncology

View full text Add to dashboard Cite

Abstract. The significance of promoter mutations of microRNAs (miRNAs) in lung cancer is poorly understood. Recent evidence demonstrated that miRNA-7 (miR-7), a unique member of the miRNA family, exhibited decreased expression and has emerged as an important regulator in lung tumorigenesis. However, the mechanism underlying the downregulation of miR-7 in lung cancer remains largely unknown. In this study, we investigated the sites of mutation of the miR-7 promoter in lung cancer tissues using DNA sequencing. We identified a G→C change at the -617 site (25/39, 64.1%) and an A→G change at the -604 site (20/39, 51.3%) in the miR-7 promoter region in lung cancer tissues. Moreover, the expression of miR-7 in cancer tissue with promoter site mutations was lower compared with that in cancer tissue without mutations (P<0.05). Furthermore, we demonstrated that mutations at these sites may decrease the activity of the miR-7 promoter and alter the expression of miR-7. Notably, mutations at these sites of the miR-7 promoter were found to be closely associated with poor prognosis of lung cancer patients (P=0.037). These data may provide novel insight on the altered expression of specific miRNA molecules in lung cancer and ultimately prove to be helpful in the development of prognostic and therapeutic strategies against lung cancer.

show abstract

Section: Promoter Mutation Is Associated With Repressed Expression Ofcontrasting

confidence: 48%

“…miRNA (miR)-7, a distinct member of the miRNA family, was reported to be able to regulate the biology of various tumor cells through repressing the expression of different target molecules (10)(11)(12)(13). miR-7 expression was found to be repressed in lung cancer tissues (7,14).…”

Section: Introductionmentioning

confidence: 99%

Promoter mutation of tumor suppressor microRNA-7 is associated with poor prognosis of lung cancer

Zhao

Wang

Liao

et al. 2015

Molecular and Clinical Oncology

View full text Add to dashboard Cite

show abstract

“…For instance, ciRS‐7, the natural antisense transcript of cerebellar degeneration‐associated protein 1 (CRD1), contained approximately 70 MREs of miR‐7 24, 32, 36. Its high expression within cytoplasms could effectively lower miR‐7 activity through binding to it, thereby down‐regulating the expressions of insulin‐like growth factor‐1 receptor (IGF1R), epidermal growth factor receptor (EGFR) and focal adhesion kinase (FAK) 37, 38, 39…”

Section: Discussionmentioning

confidence: 99%

CiRS‐7 targeting miR‐7 modulates the progression of non‐small cell lung cancer in a manner dependent on NF‐κB signalling

Zhang

et al. 2018

J Cellular Molecular Medi

108

View full text Add to dashboard Cite

The purpose of this study was to figure out the effect of ciRS‐7/miR‐7/NF‐κB axis on the development of non‐small cell lung cancer (NSCLC). In response, the expressions of ciRS‐7, miR‐7 and NF‐κB subunit (ie RELA) within NSCLC tissues and cell lines were determined with real‐time polymerase chain reaction (RT‐PCR) and Western blot. Moreover, the NSCLC cells were transfected with pcDNA3‐ciRS‐7‐ir, pcDNA3‐ciRS‐7, miR‐NC and miR‐7 mimic. Furthermore, the targeted relationships between ciRS‐7 and miR‐7, as well as between miR‐7 and RELA, were confirmed by luciferase reporter assay. The proliferation, migration and apoptosis of NSCLC cells were, successively, measured using CCK‐8 assay, wound‐healing assay and flow cytometry test. Consequently, ciRS‐7, miR‐7, histopathological grade, lymph node metastasis and histopathological stage could independently predict the prognosis of patients with NSCLC (all P < .05). Moreover, remarkably up‐regulated ciRS‐7 and RELA expressions, as along with down‐regulated miR‐7 expressions, were found within NSCLC tissues and cells in comparison with normal ones (P < .05). Besides, overexpressed ciRS‐7 and underexpressed miR‐7 were correlated with increased proliferation, migration and invasion, yet reduced apoptosis rate of NSCLC cells (P < .05). More than that, ciRS‐7 specifically targeted miR‐7 to reduce its expressions (P < .05). Ultimately, the NSCLC cells within miR‐7 + RELA group were observed with superior proliferative, migratory and invasive capabilities than those within miR‐7 group (P < .05), and RELA expression was also significantly modified by both ciRS‐7 and miR‐7 (P < .05). In conclusion, the ciRS‐7/miR‐7/NF‐kB axis could exert pronounced impacts on the proliferation, migration, invasion and apoptosis of NSCLC cells.

show abstract

“…As miRNA plays important roles in the biological activities of living cells, increasing studies have discovered that certain aberrantly expressed miRNAs in various human cancers were involved in the oncogenesis, progression and metastasis (9). Considerable miRNAs have been characterized as oncogenes or tumor suppressors by targeting downstream genes (10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

miR-886-3p upregulation in clear cell renal cell carcinoma regulates cell migration, proliferation and apoptosis by targeting PITX1

Chen

et al. 2014

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Abstract. miR-886-3p has been discovered to be involved in the oncogenesis, progression and metastasis of several types of human cancer. The aim of the present study was to identify the biological function of miR-886-3p in clear cell renal cell carcinoma (ccRCC) and to determine its possible molecular mechanisms. miR-886-3p was found to be significantly upregulated in ccRCC tissues (P<0.05), in accordance with a previous sequencing result. Functional experiments revealed that forced downregulation of miR-886-3p significantly inhibited cellular migration, suppressed cell proliferation and induced cell apoptosis of renal cancer cells. Paired-like homeodomain 1 (PITX1), which has been identified as a tumor suppressor, was found to be downregulated in ccRCC tissues and identified as a target gene of miR-886-3p. Further experiments demonstrated that the protein level, and not the mRNA level, of PITX1 was significantly decreased or increased when miR-886-3p was upregulated or downregulated, respectively, indicating that miR-886-3p acted as an oncogene by directly regulating the protein expression of PITX1 at a post-transcriptional level. In conclusion, this study revealed that miR-886-3p was upregulated in ccRCC and was involved in cellular migration, proliferation and apoptosis of renal cancer cells by directly targeting the tumor suppressor gene, PITX1.

show abstract

MicroRNA-7 Inhibits Epithelial-to-Mesenchymal Transition and Metastasis of Breast Cancer Cells via Targeting FAK Expression

Cited by 169 publications

References 50 publications

Promoter mutation of tumor suppressor microRNA-7 is associated with poor prognosis of lung cancer

Promoter mutation of tumor suppressor microRNA-7 is associated with poor prognosis of lung cancer

CiRS‐7 targeting miR‐7 modulates the progression of non‐small cell lung cancer in a manner dependent on NF‐κB signalling

miR-886-3p upregulation in clear cell renal cell carcinoma regulates cell migration, proliferation and apoptosis by targeting PITX1

Contact Info

Product

Resources

About