MicroRNA‑6852 suppresses cell proliferation and invasion via targeting forkhead box J1 in gastric cancer

Yu, Hui; Zhang, Jing; Qu, Wen; Dai, Yi; Zhang, Wanli; Li, Fen; Li, Juan

doi:10.3892/etm.2018.6569

Cited by 10 publications

(12 citation statements)

References 31 publications

(28 reference statements)

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“…GC is the third leading cause of cancer-associated mortality around the world [13]. Despite the recent improvements in GC diagnosis, surgical methods, perioperative management, and the concept of chemotherapy, GC patients still have a very low 5-year overall survival [14].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: miR-200a-3p plays tumor suppressor roles in gastric cancer cells by targeting KLF12

Jia

Zhang

Xie

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: miR-200a-3p plays tumor suppressor roles in gastric cancer cells by targeting KLF12

Jia

Zhang

Xie

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Previous reports have indicated the tumor-suppressive roles of miR-6852 in a variety of cancer types, including gastric and colorectal cancer (9)(10)(11). Therefore, it is reasonable to speculate that miR-6852 may be associated with glioma.…”

Section: Mir-6852 Expression Is Reduced In Glioma Tissues and Cellsmentioning

confidence: 96%

“…TargetScan predicted that miR-6852 has a direct binding site with LEF1. Previous studies have also indicated that miR-6852 targets forkhead box protein J1 (FOXJ1) and transcription factor 7 (TCF7) in a variety of other types of cancer (9,10). Therefore, in the present study, WT and mutant sequences of LEF1 were constructed (Fig.…”

Section: Lef1 Expression Is Inhibited By Mir-6852mentioning

confidence: 97%

MicroRNA‑6852 suppresses glioma A172 cell proliferation and invasion by targeting LEF1

et al. 2019

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microRNA (miR)-6852 has been demonstrated to suppress the progression of gastric, colorectal and cervical cancer. The mechanism by which miR-6852 regulates glioma cells is yet to be elucidated. In the present study, reverse transcription-quantitative PCR analysis was used and the results demonstrated that miR-6852 expression was reduced in glioma tissues and cells. Cell counting kit-8 and transwell assay analysis indicated that proliferation, migration and invasion of A172 cells in the miR-6852 mimic group were lower than in the miR-NC group. Compared with the Inh-NC group, A172 cells of the Inh-miR-6852 group exhibited higher proliferation, migration and invasion. Additionally, the results indicated that lymphoid enhancer binding factor 1 (LEF1) was directly inhibited by miR-6852 and LEF1 expression was negatively correlated with miR-6852 expression in glioma tissues. Furthermore, the restoration of LEF1 reversed the effects of the miR-6852 mimics. The present findings suggested that miR-6852 inhibited glioma cells proliferation, migration and invasion by targeting the suppression of LEF1. Recently, miR-6852 has been demonstrated to be associated with the development of tumors, including those in gastric, colorectal and cervical cancer (9-11). The association of miR-6852 with glioma remains undetermined. Therefore, the present study aimed to assess miR-6852 expression in glioma, and further determine the effect of miR-6852 in the regulation of glioma progression. Materials and methodsGlioma tissue collection. A total of 32 pairs of glioma tissues (14 males and 18 females; age range, 31-56 years old; median age, 47 years old) and corresponding normal tissues were collected during biopsy from Affiliated Hospital of Hebei University (Hebei, China) between October 2014 and October 2016 for the current study. Normal tissues were derived from the temporal lobes and saddle area 2 cm away from tumor tissues. All glioma tissues were obtained from patients upon first diagnosis of glioma. Among these patients, 18 cases were high-grade and 14 cases were low-grade. All patients did not recieve radiotherapy or chemotherapy prior to surgery. Patients

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“…MicroRNAs (miRNAs/miRs) can function as oncogenes or tumor suppressor genes, and regulate gene expression through translational repression or mRNA degradation by binding to the 3'-untranslated region (3'UTR) of their target mRNAs (5,6). Previous studies have identified that miRNAs exhibit aberrant expression and serve essential roles in GC (7)(8)(9)(10). Therefore, further investigation into the expression and function of miRNAs may improve understanding of the molecular mechanisms responsible for the pathogenesis of GC.…”

Section: Introductionmentioning

confidence: 99%

Upregulation of microRNA‑520a‑3p inhibits the proliferation, migration and invasion via spindle and kinetochore associated 2 in gastric cancer

Ren

Jiang

et al. 2019

Oncol Lett

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MicroRNAs (miR) serve important roles in the development and progression of tumors by targeting different genes. miR-520a-3p reported in lung and breast cancers as a tumor suppressor gene. However, the expression and functional significance of miR-520a-3p is not completely understood in gastric cancer (GC). In the present study, it was demonstrated that the expression levels of miR-520a-3p were significantly downregulated in GC tissues and cells using RT-qPCR. In addition, downregulated expression of miR-520a-3p was associated with the clinical stage of the tumor and invasion in patients with GC. Furthermore, overexpression of miR-520a-3p significantly inhibited cell proliferation, invasion and migration in SGC-7901 and MGC-803 GC cell lines using proliferation, wound healing and cell invasion assays. Spindle and kinetochore associated 2 (SKA2) was upregulated in GC cells using western blot analysis and a target gene of miR-520a-3p; miR-520a-3p mimics significantly reduced SKA2 expression. In addition, upregulation of SKA2 protein expression SKA2 reversed the miR-520a-3p-mediated inhibition of SGC-7901 cell proliferation, migration and invasion. In conclusion, miR-520a-3p functioned as a tumor suppressor gene by targeting SKA2 in GC cell lines, and may serve as a novel prognostic and potential therapeutic marker.

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MicroRNA‑6852 suppresses cell proliferation and invasion via targeting forkhead box J1 in gastric cancer

Cited by 10 publications

References 31 publications

RETRACTED ARTICLE: miR-200a-3p plays tumor suppressor roles in gastric cancer cells by targeting KLF12

RETRACTED ARTICLE: miR-200a-3p plays tumor suppressor roles in gastric cancer cells by targeting KLF12

MicroRNA‑6852 suppresses glioma A172 cell proliferation and invasion by targeting LEF1

Upregulation of microRNA‑520a‑3p inhibits the proliferation, migration and invasion via spindle and kinetochore associated 2 in gastric cancer

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