MicroRNA‑640 promotes cell proliferation and adhesion in glioblastoma by targeting Slit guidance ligand 1

Abstract

The effects of microRNAs (miRNAs/miRs) on glioblastoma have attracted the attention of researchers in the last 7 years. However, the role of miR-640 and its targeted gene, Slit guidance ligand 1 (SLIT1), in the development of glioblastoma are not yet fully understood. The present study aimed to investigate the role of miR-640 in the proliferation and adhesion of glioblastoma. Reverse transcription-quantitative PCR analysis was performed to detect miR-640 and SLIT1 expression in glioblastoma tissues and cells. In addition, the Dual-luciferase reporter and RNA-pull down assays were performed to assess the association between miR-640 and SLIT1. The Cell Counting Kit-8, BrdU ELISA, cell adhesion and caspase-3 activity assays were also performed to assess cell viability, proliferation, adhesion and apoptosis of glioblastoma cells, respectively. The results demonstrated that miR-640 expression was upregulated in glioblastoma tissues and cells. In addition, miR-640 promoted the cell viability, proliferation and adhesion of glioblastoma cells, while inhibiting cell apoptosis. SLIT1, a direct downstream target of miR-640, was demonstrated to be downregulated in glioblastoma tissues and cells. Furthermore, overexpression of SLIT1 attenuated the promotive effect of miR-640 on glioblastoma cells. Taken together, these results suggest that miR-640 accelerates the proliferation and adhesion of glioblastoma cell lines by targeting and suppressing SLIT1.

Micro RNA-640 Targeting SLIT1 Enhances Glioma Radiosensitivity by Restraining the Activation of Wnt/β-Catenin Signaling Pathway

“…In this study, we analyzed how SLIT1 affects glioma radiation tolerance. We found that SLIT1 was overexpressed in gliomas, which is inconsistent with the observations reported by Luo et al ( 32 ). The discrepancy could have occurred because our glioma tissue samples were radio-treated, while those used by Luo et al ( 32 ) were not.…”

“…We found that SLIT1 was overexpressed in gliomas, which is inconsistent with the observations reported by Luo et al ( 32 ). The discrepancy could have occurred because our glioma tissue samples were radio-treated, while those used by Luo et al ( 32 ) were not. In addition, we observed radiation-induced SLIT1 expression and detected SLIT1 silencing-induced radio-sensitization of the tested glioma cell lines.…”

Molecular Docking and Molecular Dynamics Studies Reveal Secretory Proteins as Novel Targets of Temozolomide in Glioblastoma Multiforme

“…Lastly, SLIT1, which is a secreted axon guidance molecule [ 31 ], has substantially downregulated protein and mRNA expression in GBM tissues compared to normal tissues. It is significantly suppressed by miR-640 and consequently stimulates the adhesion and proliferation of GBM cell lines, suggesting that the miR-640/SLIT1 axis might have the potential to act as a novel target for the treatment and diagnosis of GBM [ 32 ].…”