MicroRNA-590-3p enhances the radioresistance in glioblastoma cells by targeting LRIG1

Abstract

Abstract. microRNA (miR)-590 has been found to serve potential roles in cancer development; however, the expression and function of miR-590 in human gliomas remains to be elucidated. The present study aimed to investigate the expression of miR-590 in human glioma tissues and radioresistant human glioblastoma cells (U251R), and to determine the effect and related molecular mechanism of miR-590-3p on the radiosensitivity of U251R cells in vitro. The results from reverse transcription-quantitative polymerase chain reaction indicated that miR-590-3p was upregulated in human glioma tissues and radioresistant human glioblastoma cells, and that miR-590-3p expression was higher in high grade than in low grade gliomas. In vitro experiments revealed that the miR-590-3p inhibitor enhanced the radiosensitivity of U251R cells by suppressing cell viability, decreasing colony formation capacity and increasing cell apoptosis rate, as demonstrated by MTT, colony formation and flow cytometry analyses. A luciferase reporter assay demonstrated that leucine-rich repeats and immunoglobulin-like domains protein 1 (LRIG1) was a direct target of miR-590-3p. Furthermore, it was demonstrated that the effect of miR-590-3p suppression on cell viability, colony formation capacity and cell apoptosis rate was attenuated by the knockdown of LRIG1 in the U251R cells. In conclusion, the present study revealed that miR-590-3p was upregulated in human glioma tissues and radioresistant human glioblastoma cells, and miR-590-3p contributes to the radioresistance of human glioblastoma cells by directly targeting LRIG1. These findings may provide potential therapeutic strategies to prevent radioresistance in human gliomas.

Loss of PPM1F expression predicts tumour recurrence and is negatively regulated by miR‐590‐3p in gastric cancer

“…Multivariate analysis also revealed miR‐590 as an independent prognostic factor for tumour recurrence of GC patients. Intriguingly, our findings for the functions of miR‐590 in GC were also supported by some other studies in a variety of cancers, such as colorectal cancer (CRC), cervical cancer and glioblastoma, but opposed by those in CRC and glioblastoma multiforme . Thus, our above results showed that miR‐590 might act as an oncogenic marker in GC by targeting PPM1F.…”

miR-590-3p Promotes Ovarian Cancer Growth and Metastasis via a Novel FOXA2–Versican Pathway

“…For example, miR-590-3p promotes cell proliferation and invasion in T-cell acute lymphoblastic leukemia by inhibiting RB1 (6) and in colorectal cancer by targeting the Hippo pathway (7) and by promoting b-catenin signaling (32). Similarly, tumor-promoting effects of miR-590-3p were observed in glioblastoma (33). In contrast, miR-590-3p suppresses hepatocellular carcinoma growth (34) and inhibits proliferation and migration in bladder cancer cells (35).…”

The role of micro-RNA in the regulation of signal pathways in gliomas

“…Одной из мишеней miR-146b-5p является ELAVL1 (ELAV like RNA binding protein 1; также известный как HuR), что связано со снижением уровня lincRNA-p21, регулятора клеточной пролиферации и апоптоза [109]. Уровень другой микро-РНК -miR-590-3p -был повышен в тканях глиомы и радиорезистентных клетках глиобластомы, показано, что miR-590-3p способствует радиорезистентности путём прямого ингибирования опухолевого супрессора LRIG1 (leucine rich repeats and immunoglobulin like domains 1) [110].…”