MicroRNA-574 Regulates FAM210A Expression and Influences Pathological Cardiac Remodeling

Abstract

Aberrant synthesis of mitochondrial proteins impairs cardiac function and causes heart disease. However, the mechanism of regulation of mitochondria encoded protein expression during cardiac disease remains underexplored. Here, we have shown that multiple pathogenic cardiac stressors induce the expression of miR-574 guide and passenger strands (miR-574-5p/3p) in both humans and mice. miR-574 knockout mice exhibit severe cardiac disorder under heart disease-triggering stresses. miR-574-5p/3p mimics that are delivered systematically using nanoparticles reduce cardiac pathogenesis under disease insults.Transcriptome analysis of miR-574-null hearts uncovers FAM210A as a common target mRNA for both strands of miR-574. The interactome capture and translational state analyses suggest that FAM210A interacts with mitochondrial translation factors and regulates the protein expression of mitochondrial encoded electron transport chain genes. Using a human cardiomyocyte cell culture system, we discover that miR-574 regulates FAM210A expression and modulates mitochondrial encoded protein expression, which influences cardiac remodeling in heart failure.Abbreviations of key terms: ACM, adult cardiomyocytes; CF, cardiac fibroblasts; CM, cardiomyocytes; ETC, electron transport chain; FAM210A, family with sequence similarity 210 member A; HF, heart failure; ISO, isoproterenol; MEG, mitochondrial encoded gene; MI, myocardial infarction; NEMG, nuclear-encoded mitochondrial gene; RBP, RNA-binding protein; RISC, RNA-induced silencing complex; TAC, transverse aortic constriction; UTR, untranslated region Results Cardiac stress induces miR-574-5p and miR-574-3p in human and mouse heartsFrom data mining of unbiased screening data from four laboratories, the guide strand miR-574-5p has been identified as a miRNA that is robustly induced in the heart in response to pathological cardiac remodeling, which included the cardiac tissues of patients during the initial stages following MI onset 33 , a mouse model of left coronary artery occlusion-derived MI 24 , and aged murine hearts ( Fig. 1a) 26 . On the other hand, the passenger strand miR-574-3p is increased in the human hearts after MI and more remarkably induced by exercise training in murine hearts 34 . The cardiac functions of complementary strands of miR-574-5p and miR-574-3p remain unknown. miR-574 is conserved in 43 animal species (10 primates and 33 mammals, UCSC Genome Browser). In mammals, miR-574 is located in intron 1 of the host gene FAM114A1 (Family with sequence similarity 114 member A1) ( Fig. 1b). We confirmed that both miR-574-5p and miR-574-3p were significantly induced in heart tissues of chronic HF patients, with the expression level of miR-574-5p even higher than that of miR-574-3p (Fig. 1c). Northern blot analysis showed that both miR-574-5p and miR-574-3p were expressed in the normal murine heart ( Supplementary Fig. 1a). miR-574-5p and miR-574-3p were both significantly induced in isoproterenol (ISO)-or transverse aortic constriction (TAC)-treated mouse hearts, compar...

A Multi-Network Comparative Analysis of Transcriptome and Translatome Identifies Novel Hub Genes in Cardiac Remodeling

“…Atad3a is essential for mitochondrial metabolism and translation, and has been implicated in several processes in mitochondria. More recent work, which we discovered while this manuscript was under review, has shown how the miR-574- Fam210a axis regulates mitonchondrial-encoded protein expression in cardiac pathological remodeling (Wu J. et al, 2020 ). Taken together, these results suggest that Fam210a could modulate translation of mitochondrial-encoded electron-transport chain proteins, and play a yet undescribed role in cardiac muscle adaptation and growth.…”