MicroRNA-548c-3p inhibits T98G glioma cell proliferation and migration by downregulating c-Myb

Abstract

Abstract. MicroRNAs (miRNAs/miRs) are short non-coding RNAs (between 20 and 22 nucleotides) that regulate gene expression by binding to the 3'-untranslated region of target mRNA, and preventing protein translation or inducing mRNA destabilization. miRNAs are predicted to target ~60% of all mRNAs, therefore providing a marked degree of regulation of a number of cellular processes. In the present study, the expression of miR-548c-3p was determined by reverse transcription-quantitative polymerase chain reaction analysis and demonstrated to be markedly downregulated in clinical malignant glioma tissues and the glioma T98G cell line compared with normal human brain tissue. Transfection of miR-548c-3p inhibited cell proliferation by inducing G 1 cell cycle arrest and also inhibited the migration of the T98G cells in vitro. Furthermore, a bioinformatic algorithm and a luciferase reporter assay identified proto-oncogene c-Myb (c-Myb) as a potential direct target of miR-548c-3p. Further experiments demonstrated that the inhibition of c-Myb by miR-548c-3p partially mediated the antitumor effect of miR-548c-3p. The results of the present study provide the novel insight that miR-548c-3p inhibits glioma tumorigenesis by targeting c-Myb. Therefore, miR-548c-3p may contribute to the development of improved glioma treatment.

<p>The inhibitive effect of sh-HIF1A-AS2 on the proliferation, invasion, and pathological damage of breast cancer via targeting miR-548c-3p through regulating HIF-1&alpha;/VEGF pathway in vitro and vivo</p>

“…The mature miR-548c-3p is obtained from miR-548c and consists of 22 nucleotides. A mass of studies have indicated that miR-548c-3p is involved in various cancers, including prostate cancer,23 glioma,24 gastric cancer,25 and BC 26. Nevertheless, the underlying molecular mechanisms of miR-548c-3p in BC remains undiscovered.…”

NFIX Circular RNA Promotes Glioma Progression by Regulating miR-34a-5p via Notch Signaling Pathway

“…Glioma, a tumor in the central nervous system, has a poor survival rate and high mortality (Zhang Y. et al, 2017 ). The latest report suggested that the incidence of glioma has increased from 5.9/100,000 people to 6.61/100,000 people between 1973 and 2016 with the application of improved radiological diagnosis (Lu et al, 2017 ). Despite multiple treatment options, cancer remains one of the leading causes of death worldwide.…”

Analysis of circular RNA expression profiles of lung cancer in Xuanwei, China

“…Besides, this study also found that dysregulated hsa_circ_0005927 was related to the tumor size, suggesting that the expression of hsa_circ_0005927 may be related to the growth and proliferation of LCXW. What is more, the downregulated of predicted miRNA of hsa_circ_0005927 is also associated with progression of some tumors 22‐25,31 . It is worth noting that predicted miR‐592 function as a tumor suppressor in NSCLC by targeting SOX9 , 25 SOX9 is significantly upregulated in NSCLC and NSCLC cell lines 32 and promote the proliferation of lung cancer cells, 33 and SOX9 also mediates Notch1‐induced epithelial mesenchymal transformations (EMT) in lung adenocarcinoma 34 .…”