MicroRNA‑543 suppresses liver cancer growth and induces apoptosis via the JAK2/STAT3 signaling pathway

Xiu, Dianhui; Wang, Dawei; Wang, Jing; Ji, Fujian; Zhang, Wenlei

doi:10.3892/ol.2018.9838

Cited by 6 publications

(12 citation statements)

References 23 publications

(28 reference statements)

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“…The basal level of miR-543 in chemoresistant CRC cells was significantly higher than that in chemosensitive CRC cells (Figure 2C). Although several studies have revealed that miR-543 acts as an oncogene in various cancers 17-19, miR-543 has been reported to function as a tumor suppressor 20-22 . To validate the roles of miR-543 in the chemosensitivity of L-OHP, miR-543 was overexpressed in HCT116 and HCT-8 cells by directly transfecting cells with a miR-543 mimic or infecting cells with the overexpression virus.…”

Section: Resultsmentioning

confidence: 99%

Dichloroacetate Overcomes Oxaliplatin Chemoresistance in Colorectal Cancer through the miR-543/PTEN/Akt/mTOR Pathway

Lan¹,

Zhu²,

Zhu³

et al. 2019

J. Cancer

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Chemoresistance is responsible for most colorectal cancer (CRC) related deaths. In this study, we found that dichloroacetate (DCA), a pyruvate dehydrogenase kinase (PDK) inhibitor, can be used as a sensitizer for oxaliplatin (L-OHP) chemoresistant CRC cells. The aim of this study was to explore the ability of DCA to overcome L-OHP resistance in CRC cells and to identify the underlying molecular mechanisms. We found that DCA sensitizes chemoresistant CRC cells to L-OHP-induced cytotoxic effects by inhibiting clone formation capacity and promoting cell apoptosis. A microRNA (miRNA) array was used for screen, and miR-543 was identified and shown to be downregulated after DCA treatment. The expression of miR-543 was higher in chemoresistant CRC cells than in chemosensitive CRC cells. Overexpression of miR-543 increased chemoresistance in CRC cells. The validated target gene, PTEN, was negatively regulated by miR-543 both in vitro and in vivo, and PTEN was upregulated by DCA through miR-543. In addition, overexpression of miR-543 reversed the inhibition of colony formation after DCA treatment. Furthermore, the Akt/mTOR pathway is activated by miR-543 and is involved in the miR-543 induced chemoresistance. There was a significant inverse relationship between miR-543 expression and PTEN level in CRC patients, and high miR-543 expression was associated with worse prognosis. In conclusion, DCA restored chemosensitivity through miR-543/PTEN/Akt/mTOR pathway, and miR-543 may be a potential marker or therapeutic target for chemoresistance in CRC.

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Section: Resultsmentioning

confidence: 99%

Dichloroacetate Overcomes Oxaliplatin Chemoresistance in Colorectal Cancer through the miR-543/PTEN/Akt/mTOR Pathway

Lan¹,

Zhu²,

Zhu³

et al. 2019

J. Cancer

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“…However, Fan et al (2016) found that miR‐543 expression was downregulated in CRC tissue samples and had a tumor‐suppressive effect. L. P. Liu, Yang, et al (2017) and Xiu, Wang, Wang, Ji, and Zhang (2019) confirmed the low expression of miR‐543 in tissue samples of hepatocellular carcinoma (HCC), while Visalli et al (2018) found upregulation of miR‐543 expression in tissue samples from patients with HCC. Dang, Zheng, Liu, Wu, and Wu (2017) found that the expression level of miR‐543 was upregulated in cervical cancer tissue, while X. Liu, Gan, and Zhang (2019) and Ma et al (2020) found that miR‐543 expression was downregulated in cervical cancer tissue and cells.…”

Section: The Role Of Mir‐543 In Human Cancermentioning

confidence: 97%

“…L. P Liu, Yang, et al (2017). andXiu, Wang, Wang, Ji, and Zhang (2019) confirmed the low expression of miR-543 in tissue samples of hepatocellular carcinoma (HCC), whileVisalli et al (2018) found upregulation of miR-543 expression in tissue samples from patients with HCC Dang, Zheng, Liu, Wu, and Wu (2017). found that the expression level of miR-543 was upregulated in cervical cancer tissue, while X Liu, Gan, and Zhang (2019).…”

mentioning

confidence: 88%

The role of miR‐543 in human cancerous and noncancerous diseases

Zhou

Zhao

Duan

2020

Journal Cellular Physiology

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MicroRNA (miRNA) is a noncoding single-stranded RNA molecule that can regulate the posttranscriptional expression level of a gene by binding to the 3′-untranslated region (3′-UTR) of the target messenger RNA. miR-543 is a kind of miRNA, which plays an important role in the occurrence and development of various human cancerous and noncancerous diseases. miR-543 directly or indirectly regulates a large number of downstream target genes and plays an important role in cellular components, biological processes, and molecular functions. In addition, many studies have verified the regulatory mechanism, physiological role, biological function, and prognostic value of miR-543. Therefore, this article reviews the papers published in the past decade and elaborates on the research progress of miR-543 from the aspects of physiology and pathology, especially in cancerous and other noncancerous diseases. In particular, we pay attention to the expression patterns, direct targets, biological functions, related pathways, and prognostic value of miR-543 reported in experimental articles. And by comparing similar research articles, we point out existing controversies in this field to date, so as to facilitate further research in the future.

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“…Inhibition of miRNA-543 expression resulted in liver cancer cells with exactly the same consequences like inhibition of miRNA-409: increased cancer cell proliferation and decreased apoptosis. It also activated the protein expression of phosphorylated JAK2, phosphorylated STAT3, c-Myc, and B-cell lymphoma 2 (Bcl-2) in liver cancer cells [53]. miRNA-3662, which downregulated in HCC tissues and cell lines, may be involved in reprogramming cancer cells' glucose metabolism and forming of Warburg effect while having hypoxia-inducible factor-1α (HIF-1α) as one of the direct targets.…”

Section: Mirna Expression Patterns In Hccmentioning

confidence: 99%

miRNAs in Liver Cancer

Petkevich¹,

Abramov²,

Pospelov³

2023

Liver Cancer - Genesis, Progression and Metastasis

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miRNAs are small noncoding RNAs, which are involved in epigenetic regulation of gene expression. Hepatocellular carcinoma (HCC), although not being at the top of most widespread cancers, nevertheless, remains among cancers with the most lethal cases. The chapter is dedicated to the epigenetic aspect of HCC development, namely the role of miRNA in this process. Being small and noncoding RNAs, they have a huge and significant function in gene regulation. This chapter will briefly cover following questions: miRNA biogenesis and function, metabolic and signaling pathways disrupted in HCC with a significant miRNA involvement, and main miRNAs contributing to HCC development and their targets.

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MicroRNA‑543 suppresses liver cancer growth and induces apoptosis via the JAK2/STAT3 signaling pathway

Cited by 6 publications

References 23 publications

Dichloroacetate Overcomes Oxaliplatin Chemoresistance in Colorectal Cancer through the miR-543/PTEN/Akt/mTOR Pathway

Dichloroacetate Overcomes Oxaliplatin Chemoresistance in Colorectal Cancer through the miR-543/PTEN/Akt/mTOR Pathway

The role of miR‐543 in human cancerous and noncancerous diseases

miRNAs in Liver Cancer

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