MicroRNA‐524‐5p suppresses the progression of papillary thyroid carcinoma cells via targeting on FOXE1 and ITGA3 in cell autophagy and cycling pathways

Abstract

MicroRNAs are beneficial for cancer therapy as they can simultaneously downregulate multiple targets involved in diverse biological pathways related to tumor development. In papillary thyroid cancer, many microRNAs were identified as differentially expressed factors in tumor tissues. In another way, recent studies revealed cell proliferation, cell cycling, apoptosis, and autophagy are critical pathways controlling papillary thyroid cancer development and progression. As miR‐524‐5p was approved as a cancer suppressor targeting multiple genes in several types of cancer cells, this study aims to characterize the role of miR‐524‐5p in the thyroid cancer cell. The expression of miR‐524‐5p was decreased in the papillary thyroid cancer tissues and cell lines, while forkhead box E1 (FOXE1) and ITGA3 were increased. In the clinical case, expression of miR‐524‐5p, FOXE1, and ITGA3 were significantly correlated with papillary thyroid cancer development and progression. FOXE1 and ITGA3 were approved as direct targets of miR‐524‐5p. miR‐524‐5p could inhibit papillary thyroid cancer cell viability, migration, invasion, and apoptosis through targeting FOXE1 and ITGA3. Cell cycling and autophagy pathways were disturbed by downregulation of FOXE1 and ITGA3, respectively. Collectively, miR‐524‐5p targeting on FOXE1 and ITGA3 prevents thyroid cancer progression through different pathways including cell cycling and autophagy.

miR‑122‑5p suppresses the oncogenesis of PTC by inhibiting DUSP4 expression

“…In addition, miR-215 can suppress the proliferation, migration and invasion of PTC cells (4). Similar reports also confirmed the inhibitory effect of miR-let-7e, miR-200b, miR-188-5p and miR-524-5p on PTC oncogenesis (20)(21)(22)(23). In the present study, miR-122-5p was focused on, which is widely reported to suppress the growth and function of tumours (5)(6)(7) and specifically bind to the 3'-UTR of PTC promoters (7).…”

“…Nude mouse xenograft experiments revealed that miR-122-5p overexpression suppressed PTC growth in vivo. Several miRNAs have been revealed to inhibit the carcinogenesis of PTC, such as miR-let-7e and miR-200b (20)(21)(22)(23). These data indicated that miR-122-5p may have a potential therapeutic effect on PTC.…”

Development and validation of a nomogram with an autophagy-related gene signature for predicting survival in patients with glioblastoma

“…This subunit joins with a beta 1 subunit to form an integrin that functions as a cell surface adhesion molecule and interacts with extracellular matrix proteins. The literature reports that high expression of ITGA3 can promote proliferation, progression and invasion in various tumors, such as cholangiocarcinoma, thyroid carcinoma, pancreatic adenocarcinoma and glioma [31][32][33]. Fiscon et al [33] demonstrated that ITGA3 was involved in ECM-receptor interactions and focal adhesion pathways and promoted the development and differentiation of GBM cells.…”

The Emerging Roles of Autophagy-Related MicroRNAs in Cancer

“…In NSCLC, miR-18a-5p was reported to promote autophagy by targeting interferon regulatory factor 2 (IRF2) to increase apoptosis while inhibiting cellular migration 121 . MiR-524-5p, a downregulated miRNA in papillary thyroid carcinoma, was shown to promote autophagy and inhibit cell viability, invasion, migration and apoptosis by targeting ITGA3 and FOXE1 in papillary thyroid cancer 136 .…”