MicroRNA-513c-5p is involved in the pathogenesis of preeclampsia by regulating of low-density lipoprotein receptor-associated protein 6

Zhou, Qian; Li, Hongyan; Zhang, Yan; Peng, Wei; Hou, Haiyan; Gu, Mengqi; Zhang, Fengyuan; Wang, Xietong; Gu, Xiao; Li, Lei

doi:10.1186/s12884-021-04069-w

Cited by 4 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MicroRNA (miRNA) is a type of widely existing small molecule non-coding RNA, playing a role in cell proliferation, differentiation, apoptosis, and other processes [14,15]. The expression of miRNA is tissue-speci c, and its abnormal expression may be correlated with the occurrence and development of a wide variety of diseases.PE refers to a placenta-derived disease.…”

Section: Discussionmentioning

confidence: 99%

Expression of miR-377-3p in preeclampsia and its effect on CCNG2

Niu,

Yu,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

Background To investigate the relationship between changes in miR-377-3p and cell cyclin G2 (CCNG2) expression and clinical features of pregnant women in preeclampsia (PE) and to further analyze the effect of miR-377-3p on CCNG2. Methods 120 pregnant subjects were recruited consisting of 70 PE and 50 healthy pregnancies. The expression of miR-377-3p and CCNG2 mRNA were detected through RT-qPCR, and the protein expression of CCNG2 was examined through Western Blot. The Pearson test was used to analyze the relationship between miR-377-3p and CCNG2 mRNA expression and clinical parameters related to pregnant women with PE, and the association between them. Dual luciferase assay to verify the effect of miR-377-3p on CCNG2. Results The expression of miR-377-3p in the peripheral plasma and placenta of pregnant women in the PE group was lower than that in the control group, and the expression levels of CCNG2 mRNA and protein were higher than that in the control group. Expression of miR-377-3p and CCNG2 mRNA in plasma and placenta of pregnant women with PE was correlated with systolic, diastolic blood pressure and neonatal birth weight. miR-377-3p and CCNG2 were consistently expressed in plasma and placental tissues, and their expressions were negatively correlated. Dual luciferase assay showed that miR-377-3p negatively regulated CCNG2 expression in HTR-8/SVneo cells. Conclusion Aberrant expression of miR-377-3p and CCNG2 in pregnant women with PE was associated with the severity of PE and its prognosis. It is hypothesized that miR-377-3p may be involved in the pathogenesis of PE through targeted inhibition of CCNG2 expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of miR-377-3p in preeclampsia and its effect on CCNG2

Niu,

Yu,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The four potential biomarker miRNAs identified in our study have never been linked to RPL before. The expression of miR-145-5p was increased in the placentas of patients with PE (Zhou et al, 2021). Mir-145-5p overexpression inhibited HTR-8/SVneo cell motility and invasion by negatively regulating Cyr61 expression through interactions with its 3′-untranslated region (UTR) (Wen et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of circulating microRNAs and the placental transcriptome in recurrent pregnancy loss

Zhou

Shi

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

Recurrent pregnancy loss (RPL) is a major type of pathological pregnancy that still lacks reliable early diagnosis and effective treatment. The placenta is critical to fetal development and pregnancy success because it participates in critical processes such as early embryo implantation, vascular remodeling, and immunological tolerance. RPL is associated with abnormalities in the biological behavior of placental villous trophoblasts, resulting in aberrant placental function. MicroRNAs (miRNAs) are increasingly being recognized as essential regulators of placental development, as well as potential biomarkers. In this study, plasma miRNAs and placental messenger RNAs (mRNAs) from RPL patients and normal pregnant (NP) controls were sequenced and analyzed. Compared to those in NP controls, 108 circulating miRNAs and 1199 placental mRNAs were differentially expressed in RPL samples. A total of 140 overlapping genes (overlapping between plasma miRNA target genes and actual placental disorder genes) were identified, and functional enrichment analysis showed that these genes were mainly related to cell proliferation, angiogenesis, and cell migration. The regulatory network among miRNAs, overlapping genes, and downstream biological processes was analyzed by protein–protein interactions and Cytoscape. Moreover, enriched mRNAs, which were predictive targets of the differentially expressed plasma miRNAs miR-766-5p, miR-1285-3p, and miR-520a-3p, were accordingly altered in the placenta. These results suggest that circulating miRNAs may be involved in the pathogenesis of RPL and are potential noninvasive biomarkers for RPL.

show abstract

“…miR-513c-5p is a coding product of MIR-513C , which is localized on the human chromosome X. Little is known of miR-513c-5p function, except for some reports indicating that miR-513c-5p might play roles in the progression of thyroid cancer, neuroblastoma, or preeclampsia ( Jia et al, 2020 ; Tong et al, 2021 ; Zhou et al, 2021 ). Our intensive functional and mechanistic investigation demonstrated that miR-513c-5p effectively inhibited caspase-1 expression by binding to its 3′ untranslated region (3′UTR).…”

Section: Introductionmentioning

confidence: 99%

miR-513c-5p Suppression Aggravates Pyroptosis of Endothelial Cell in Deep Venous Thrombosis by Promoting Caspase-1

Chu

Wang

Zhang

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Deep vein thrombosis (DVT) is a common peripheral vascular disease. Secondary pulmonary embolism (PE) caused by DVT leads to substantial patient death. Inflammation has been suggested as a key factor in the pathophysiology of DVT, however, involvement of pyroptosis-related inflammatory factors in DVT formation remains unclear. Here, we proposed that post-transcriptional modification of caspase-1 might be a crucial trigger for enhanced pyroptosis in vascular endothelial cells (VECs), and consequently contributed to severer symptoms in DVT patients. In order to explore the involvement of pyroptosis in DVT, peripheral blood mononuclear cells were collected from 30 DVT patients, and compared with the healthy controls, we found caspase-1 was increased both in mRNA and protein levels. miRNA microarray analysis demonstrated that down-regulated miR-513c-5p was significantly negatively correlated with the expression of caspase-1. In vitro assays suggested that miR-513c-5p overexpression could ameliorate the expression of caspase-1, and thus decreased the production of cleaved gasdermin D (GSDMD) and interleukin (IL)-1β and IL-18 in VECs. The dual-luciferase reporter assay identified direct binding between miR-513c-5p and the 3′ untranslated region of caspase-1 encoding gene. The administration of miR-513c-5p mimics through tail vein injection or caspase-1 inhibitor (vx-765) by intraperitoneal injection remarkably decreased the volume of blood clots in vivo, whereas miR-513c-5p inhibitor aggravated thrombosis formation and this effect was dramatically weakened when treated in combination with vx-765. Collectively, these results revealed that the pyroptosis of VECs induced by decreased miR-513c-5p was involved in DVT progression and indicated a potential therapeutic strategy of targeting the miR-513c-5p/caspase-1/GSDMD signal axis for DVT management.

show abstract

MicroRNA-513c-5p is involved in the pathogenesis of preeclampsia by regulating of low-density lipoprotein receptor-associated protein 6

Cited by 4 publications

References 31 publications

Expression of miR-377-3p in preeclampsia and its effect on CCNG2

Expression of miR-377-3p in preeclampsia and its effect on CCNG2

Integrative analysis of circulating microRNAs and the placental transcriptome in recurrent pregnancy loss

miR-513c-5p Suppression Aggravates Pyroptosis of Endothelial Cell in Deep Venous Thrombosis by Promoting Caspase-1

Contact Info

Product

Resources

About