MicroRNA-506-3p inhibits osteosarcoma cell proliferation and metastasis by suppressing RAB3D expression

Wang, Jiashi; Qiu, Chuang; Zhenjun, Zhang; Wang, Guangbin; B, Li; He, Ming

doi:10.18632/aging.101468

Cited by 51 publications

(38 citation statements)

References 26 publications

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“…RAB3D involvement has been implicated in multiple regulatory processes, such as vesicle transport, protein secretion, and signal transduction (63,64). It was reported that RAB3D is highly expressed in tumor tissues and cells, and promotes the migration and invasion of tumor cells (65,66). In our study, we found that RAB3D can act as an oncogene in glioma, and hsa_circ_0088732 can regulate RAB3D expression by sponging miR-661.…”

Section: Discussionsupporting

confidence: 54%

Lcn2-derived Circular RNA (hsa_circ_0088732) Inhibits Cell Apoptosis and Promotes EMT in Glioma via the miR-661/RAB3D Axis

Jin

Liu

et al. 2020

Front. Oncol.

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Background: Glioma is the most common malignant tumor of the central nervous system, and often displays invasive growth. Recently, circular RNA (circRNA), which is a novel non-coding type of RNA, has been shown to play a vital role in glioma tumorigenesis. However, the functions and mechanism of lipocalin-2 (Lcn2)-derived circular RNA (hsa_circ_0088732) in glioma progression remain unclear. Methods: We evaluated hsa_circ_0088732 expression by fluorescence in situ hybridization (FISH), Sanger sequencing, and PCR assays. Cell apoptosis was evaluated by flow cytometry and Hoechst 33258 staining. Transwell migration and invasion assays were performed to measure cell metastasis and viability. In addition, the target miRNA of hsa_circ_0088732 and the target gene of miR-661 were predicted by a bioinformatics analysis, and the interactions were verified by dual-luciferase reporter assays. RAB3D expression was analyzed by an immunochemistry assay, and E-cadherin, N-cadherin, and vimentin protein expression were examined by western blot assays. A mouse xenograft model was developed and used to analyze the effects of hsa_circ_0088732 on glioma growth in vivo. Results: We verified that hsa_circ_0088732 is circular and highly expressed in glioma tissues. Knockdown of hsa_circ_0088732 induced glioma cell apoptosis and inhibited glioma cell migration, invasion, and epithelial-mesenchymal transition (EMT). We found that hsa_circ_0088732 negatively regulated miR-661 by targeting miR-661, and RAB3D was a target gene of miR-661. In addition, inhibition of miR-661 promoted glioma cell metastasis and suppressed cell apoptosis. Knockdown of RAB3D induced cell apoptosis and suppressed cell metastasis. Moreover, hsa_circ_0088732 accelerated glioma progression through its effects on the miR-661/RAB3D axis. Finally, results from a mouse xenograft model confirmed that knockdown of hsa_circ_0088732 induced miR-661 expression, resulting in suppression of RAB3D expression and inhibition of tumor growth in vivo. Jin et al. hsa_circ_0088732 Acts as an Oncogene in Glioma Conclusion: We demonstrated that hsa_circ_0088732 facilitated glioma progression by sponging miR-661 to increase RAB3D expression. This study provides a theoretical basis for understanding the development and occurrence of glioma, as well as for the development of targeted drugs.

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Section: Discussionsupporting

confidence: 54%

Lcn2-derived Circular RNA (hsa_circ_0088732) Inhibits Cell Apoptosis and Promotes EMT in Glioma via the miR-661/RAB3D Axis

Jin

Liu

et al. 2020

Front. Oncol.

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“…miRNAs are important regulators for tumorigenesis and it was proved that miRNAs have the potential to be used as novel therapeutic methods for cancer (10)(11)(12)(13)15). Rab3D, one member of the Rab GTPase family, serves an oncogenic role in several cancer types including osteosarcoma, esophageal squamous cell carcinoma and CRC (16)(17)(18). Evidence demonstrated that Rab3D is a key player in regulating tumor metastasis, which suggested that silencing Rab3D expression may be a way to restrain cancer metastasis (19).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D

et al. 2018

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The purpose of the present study was to investigate the biological role of microRNA-761 (miR-761) in colorectal cancer (CRC) and the underlying mechanisms by which miR-761 regulates CRC cell proliferation and migration. Quantitative polymerase chain reaction was performed to measure miR-761 expression in CRC tumor tissues and cell lines. It was demonstrated that miR-761 expression was dramatically reduced in CRC tumor tissues and cell lines compared with in normal tissues and cell lines. Overexpression of miR-761 significantly decreased CRC cell growth and migration. Using bioinformatics analysis and luciferase reporter assays, Rab3D was identified as a novel target of miR-761. In addition, it was demonstrated that Rab3D expression was negatively correlated with miR-761. Furthermore, overexpression of Rab3D could reverse the inhibitory effects of miR-761 on cell proliferation and migration. Collectively, the present study demonstrated that miR-761 overexpression could inhibit the proliferation and migration of CRC cell lines, partly at least, via directly targeting Rab3D.

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“…For example, Wu et al revealed that MiRNA-145-3p was reported as a tumour suppressor, inhibiting proliferation and induces apoptosis and autophagy of OS cell [20]. miR-506-3p is a tumour suppressor by repressing the proliferation and metastasis of OS cell [21]. miR-223-3p was reported to suppress OS metastasis by targeting CDH6 [19].…”

Section: Introductionmentioning

confidence: 99%

miR-219a-5p represses migration and invasion of osteosarcoma cells via targeting EYA2

Zhu

Chen

Lin

2018

Artificial Cells, Nanomedicine, and Biotechnology

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MicroRNA-506-3p inhibits osteosarcoma cell proliferation and metastasis by suppressing RAB3D expression

Cited by 51 publications

References 26 publications

Lcn2-derived Circular RNA (hsa_circ_0088732) Inhibits Cell Apoptosis and Promotes EMT in Glioma via the miR-661/RAB3D Axis

Lcn2-derived Circular RNA (hsa_circ_0088732) Inhibits Cell Apoptosis and Promotes EMT in Glioma via the miR-661/RAB3D Axis

MicroRNA‑761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D

miR-219a-5p represses migration and invasion of osteosarcoma cells via targeting EYA2

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