MicroRNA-499 Expression Distinctively Correlates to Target Genes sox6 and rod1 Profiles to Resolve the Skeletal Muscle Phenotype in Nile Tilapia

Nachtigall, Pedro Gabriel; Dias, Marianne Camargos; Carvalho, Robson Francisco; Martins, Cesar; Pinhal, Danillo

doi:10.1371/journal.pone.0119804

Cited by 38 publications

(35 citation statements)

References 47 publications

(61 reference statements)

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“…The transcription factor SOX6 is known to modulate terminal differentiation in mesoderm-, ectoderm-, and endoderm-derived cell lineages. [40][41][42] Our data show that SOX6 protein is predominantly expressed in the proliferating basal layer of the healthy epidermis, in which miR-335 expression is low. In AD lesional skin, in which miR-335 expression is lost, the SOX6 expression domain expands into the suprabasal layers.…”

Section: Discussionmentioning

confidence: 64%

Belinostat resolves skin barrier defects in atopic dermatitis by targeting the dysregulated miR-335:SOX6 axis

Liew

Sundaram

Quah

et al. 2020

Journal of Allergy and Clinical Immunology

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Section: Discussionmentioning

confidence: 64%

Belinostat resolves skin barrier defects in atopic dermatitis by targeting the dysregulated miR-335:SOX6 axis

Liew

Sundaram

Quah

et al. 2020

Journal of Allergy and Clinical Immunology

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“…Another molecule involved in muscle growth is srf ( serum response factor ), the mRNA of which is silenced by miR-133a and miR-133b, which also belong to the same family of miRNAs and are related to myoblast proliferation [ 12 , 18 ]. In addition to these miRNAs, miR-499 is involved in the specification and maintenance of the slow-twitch phenotype in muscle fibers, particularly by silencing sox6 (( sex determining region Y)-box 6 ), which participates in the definition of the fast-twitch phenotype [ 13 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Differential microRNA Expression in Fast- and Slow-Twitch Skeletal Muscle of Piaractus mesopotamicus during Growth

et al. 2015

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Pacu (Piaractus mesopotamicus) is a Brazilian fish with a high economic value in pisciculture due to its rusticity and fast growth. Postnatal growth of skeletal muscle in fish occurs by hyperplasia and/or hypertrophy, processes that are dependent on the proliferation and differentiation of myoblasts. A class of small noncoding RNAs, known as microRNAs (miRNAs), represses the expression of target mRNAs, and many studies have demonstrated that miR-1, miR-133, miR-206 and miR-499 regulate different processes in skeletal muscle through the mRNA silencing of hdac4 (histone deacetylase 4), srf (serum response factor), pax7 (paired box 7) and sox6 ((sex determining region Y)-box 6), respectively. The aim of our work was to evaluate the expression of these miRNAs and their putative target mRNAs in fast- and slow-twitch skeletal muscle of pacu during growth. We used pacus in three different development stages: larval (aged 30 days), juvenile (aged 90 days and 150 days) and adult (aged 2 years). To complement our study, we also performed a pacu myoblast cell culture, which allowed us to investigate miRNA expression in the progression from myoblast proliferation to differentiation. Our results revealed an inverse correlation between the expression of the miRNAs and their target mRNAs, and there was evidence that miR-1 and miR-206 may regulate the differentiation of myoblasts, whereas miR-133 may regulate the proliferation of these cells. miR-499 was highly expressed in slow-twitch muscle, which suggests its involvement in the specification of the slow phenotype in muscle fibers. The expression of these miRNAs exhibited variations between different development stages and between distinct muscle twitch phenotypes. This work provides the first identification of miRNA expression profiles in pacu skeletal muscle and suggests an important role of these molecules in muscle growth and in the maintenance of the muscle phenotype.

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“…Intriguingly, miR-499 was found to be expressed in skeletal muscle, Wang et al reported that miRNA-208a, another muscle-enriched miRNA, had a high specificity (100%) and sensitivity (90.9%) in the diagnosis of AMI, however, the sensitivity of miRNA-499 was only 36.4% (25,26). Thus, whether miRNA-499 can be a highly © Annals of Translational Medicine.…”

Section: Possibility Of Mir-499 As a Biomarker For Diagnosis Of Amimentioning

confidence: 99%

Circulating miR-499 as a potential biomarker for acute myocardial infarction

Xin¹,

Yang²,

Han³

2016

Ann. Transl. Med.

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Acute myocardial infarction (AMI), a common heart disease that may lead to chronic heart failure, is the leading cause of morbidity and mortality worldwide. MicroRNAs (miRNAs) are small non-coding RNAs that mediate the expression of target genes. Recently, a number of miRNAs are emerging as potential biomarkers of AMI. MiRNA-499 is a newly discovered member of miRNAs, and is mainly expressed in myocardium, the circulating levels of miRNA-499 was increased in AMI patients. This review summarizes the latest advances in the miRNA-499 study and discusses the potential of miRNA-499 to be a biomarker of AMI. Acute myocardial infarction (AMI) is one of the leading causes of morbidity and mortality worldwide, thus an effective and accurate diagnostic biomarker would be needed to help decrease the mortality of AMI patients. One of the traditional biomarkers for AMI is troponin. Circulating troponin levels rise around 3 hours after the onset of chest pain, due to the relative delayed release time of troponin. It is widely believed that a rapid and correct diagnosis of AMI has an important impact on patients' treatment and prognosis. Therefore, earlier biomarkers than troponin with both high sensitivity and specificity remain to be needed. MicroRNAs (miRNAs) are endogenous small non-coding RNAs of less than 22 nucleotides that are implicated in nearly all cellular events including cell differentiation and proliferation as well as the pathogenesis of certain human diseases (1,2). Recently, a number of findings support the notion of the implication of miRNAs in the development and progression of cardiovascular diseases (3). The plasma levels of miRNA-1, -133a, -133b, and -499-5p are upregulated in AMI patient, and represent novel biomarkers of cardiac damage. Several reviews have provided an over-view of biology of miRNA-1, miRNA-133b, -499-5p and their involvement in cardiovascular physiology and pathology, and discussed the potential of these miRNAs to become a novel biomarker of AMI and even an effective therapeutics agent (4-8). miRNA-499 is a newly discovered member of miRNAs encoded by myosin gene family, and its expression level in plasma was elevated in the patients with AMI, a common heart disease that is the leading cause of morbidity and mortality worldwide (9). A previous study compared the diagnostic performance of miRNA-499 and some traditional biomarkers like SMB, cTnI, cTnT, CK-MB, CK, LDH, and concluded that miRNA-499 was present in plasma earlier than other conventional biomarkers of AMI, pointing to the likelihood that miR-499 can be a vital molecule in the early diagnosis of AMI. This study also found that the circulating level of miR-499 correlated well with circulating troponin I

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MicroRNA-499 Expression Distinctively Correlates to Target Genes sox6 and rod1 Profiles to Resolve the Skeletal Muscle Phenotype in Nile Tilapia

Cited by 38 publications

References 47 publications

Belinostat resolves skin barrier defects in atopic dermatitis by targeting the dysregulated miR-335:SOX6 axis

Belinostat resolves skin barrier defects in atopic dermatitis by targeting the dysregulated miR-335:SOX6 axis

Differential microRNA Expression in Fast- and Slow-Twitch Skeletal Muscle of Piaractus mesopotamicus during Growth

Circulating miR-499 as a potential biomarker for acute myocardial infarction

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