MicroRNA-497 is a potential prognostic marker in human cervical cancer and functions as a tumor suppressor by targeting the insulin-like growth factor 1 receptor

Luo, Min; Shen, Dongxiang; Zhou, Xiaolu; Chen, Xiaodong; Wang, Wei

doi:10.1016/j.surg.2012.12.004

Cited by 110 publications

(99 citation statements)

References 30 publications

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“…The current study focused on miRNA-497, which was previously demonstrated to exhibit decreased expression in numerous tumor types, and which may function as a tumor suppressor (12)(13)(14)(15)(16)(17)(18)(19)(20). The results confirmed that the expression levels of miRNA-497 are decreased in HCC tumor tissues or HCC-derived cell lines compared with adjacent non-cancerous tissues or normal human L02 hepatocytes.…”

Section: Discussionsupporting

confidence: 74%

“…IGF-1R, a target gene of miR-497, was also selected for investigation; overexpression of this gene has been reported in human cervical cancer and colorectal cancer (12,20). Furthermore, several online databases, including miRanda (http://www.microrna.org/microrna/getMrna.do?gene=3480 &utr=31402&organism=9606&matureName=hsa-miR-497) and miRTarBase (http://mirtarbase.mbc.nctu.edu.tw/php/ search.php?q=search_exact&searchword=hsa-miR-497-5p), indicated that IGF-1R mRNA contains miR-497 binding sites.…”

Section: Discussionmentioning

confidence: 99%

“…Many of these miRNAs act as tumor suppressors and/or oncogenes, and are involved in cell activities that include development, differentiation, proliferation, apoptosis, metabolism and immunity (7,10,11). Recently, an increasing number of studies have revealed that microRNA-497 (miR-497) levels are decreased in tumors, and that it functions as a tumor suppressor in a number of types of human cancer, including colorectal, gastric, cervical and breast cancers, adrenocortical carcinoma and melanoma (12)(13)(14)(15)(16)(17)(18)(19)(20). Similarly, a study by Furuta et al (21) indicated that miR-497 targets multiple cell cycle regulators and suppresses cell cycle progression in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…IGF-1R mRNA and protein expression are increased in HCC and are closely associated with the progression of malignant tumors (34,35). To date, studies have demonstrated that miR-497 targets IGF-1R and has a tumor suppressive role in human cervical cancer (20) and in colorectal cancer (12). However, whether miR-497 functions as a tumor suppressor by directly targeting IGF-1R in HCC remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma

Ding

et al. 2015

Oncology Letters

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Abstract. Hepatocellular carcinoma (HCC) is the most common primary cancer of the liver. ) is known to be downregulated in several types of human cancer; however, the expression, function and underlying mechanisms of miR-497 in HCC remain unclear. Therefore, the present study investigated miR-497 expression in HCC samples and HCC-derived cell lines using reverse transcription-quantitative polymerase chain reaction. The protein expression of one of the predicted common targets of miR-497, insulin-like growth factor-1 receptor (IGF-1R), was assessed using western blot analyses and immunohistochemistry. The role of miR-497 in regulating the proliferation of HCC-derived cells was also investigated in vitro and in vivo. Of 60 paired specimens from HCC patients, miR-497 was downregulated in 42 cancer specimens compared with adjacent non-cancer tissues. Western blotting and immunohistochemical analyses revealed that IGF-1R expression was significantly increased in HCC compared to control tissues. In addition, overexpression of miR-497 was observed to inhibit colony formation and tumor growth in MHCC-97H human HCC cells. Conversely, SMMC-7721 human HCC cells transfected with a miR-497 inhibitor exhibited enhanced colony formation and tumor growth. Finally, IGF-1R protein, phosphoinositide 3-kinase/Akt signaling pathway-associated proteins and cyclin pathway-associated proteins were differentially expressed between miR-497-overexpressing cells and miR-497-silenced cells. These results indicate that miR-497 may be a potentially effective gene therapy target.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma

Ding

et al. 2015

Oncology Letters

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“…Zheng et al also reported that miR-497 simultaneously inhibits protein levels of three members of the ERK pathway, RAF1, MEK1 and ERK1 in Hela cells (Zheng et al, 2012). Guo, et al and Luo, et al showed that miR-497 could target the insulin-like growth factor 1 receptor (IGF-1R) and had a tumor-suppressive role in human colorectal and cervical cancer (Guo et al, 2012;Luo et al, 2013). miR-497 maybe have a tumor suppressive role in prostate cancer cells by targeting MAPK, IGF-1R or other signaling pathways, which we will prove them next step.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-497 Suppresses Proliferation and Induces Apoptosis in Prostate Cancer Cells

Wang

Li²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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A systematic study on dysregulated microRNAs in cervical cancer development

Lin

Ding

et al. 2015

Intl Journal of Cancer

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MicroRNAs (miRNAs) are short regulatory RNAs that modulate the transcriptome and proteome at the post-transcriptional level. To obtain a better understanding on the role of miRNAs in the progression of cervical cancer, meta-analysis and gene set enrichment analysis were used to analyze published cervical cancer miRNA studies. From 85 published reports, which include 3,922 cases and 2,099 noncancerous control tissue samples, 63 differentially expressed miRNAs (DEmiRNAs) were identified in different stages of cervical cancer development (CIN 1-3 and CC). It was found that some of the dysregulated miRNAs were associated with specific stages of cervical cancer development. To illustrate the impact of miRNAs on the pathogenesis of cervical cancer, a miRNA-mRNA interaction network on selected pathways was built by integrating viral oncoproteins, dysregulated miRNAs and their predicted/validated targets. The results indicated that the deregulated miRNAs at the different stages of cervical cancer were functionally involved in several key cancer related pathways, such as cell cycle, p53 and Wnt signaling pathways. These dysregulated miRNAs could play an important role in cervical cancer development. Some of the stage-specific miRNAs can also be used as biomarkers for cancer classification and monitoring the progression of cancer development.Cervical cancer is the second leading cause of female cancer deaths worldwide, with an estimated annual global incidence of more than half a million new cases and about 270,000 deaths. 1,2 Most cervical cancers are caused by infection with high risk (HR) human papilloma virus (HPV), which disrupts the normal proliferation and differentiation of cervical squamous epithelium cells via two viral-encoded oncoproteins: E6 and E7. The E6/E7 protein tumorgenesis is mediated through the interaction with cellular tumor suppressor proteins, TP53 and RB1 (Retinoblastoma 1), respectively. 3 Besides the misregulated host proteins, E6 and E7 oncoproteins also interact with host noncoding transcripts such as microRNAs (miRNAs). [4][5][6] MiRNAs are short (19-25 nucleotides in length) regulatory RNAs that modulate gene expression level by partial base pairing with the 3' untranslated region of their target messenger RNAs (mRNAs). 7 There are currently over 2,500 human miRNAs recorded in miRBase (www.mirbase.org), and it has been estimated that roughly two third of human transcripts are regulated by miRNAs. 8 One of the most important features of miRNAs is the partial nucleotide sequence paring between the miRNA and its mRNA target, which results in promiscuous interactions: one miRNA often interacts with more than one mRNAs, and one transcript can be targeted by multiple miRNAs. 9 The first cervical cancer

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MicroRNA-497 is a potential prognostic marker in human cervical cancer and functions as a tumor suppressor by targeting the insulin-like growth factor 1 receptor

Cited by 110 publications

References 30 publications

MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma

MicroRNA-497 regulates cell proliferation in hepatocellular carcinoma

MicroRNA-497 Suppresses Proliferation and Induces Apoptosis in Prostate Cancer Cells

A systematic study on dysregulated microRNAs in cervical cancer development

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