MicroRNA-494 promotes cervical cancer proliferation through the regulation of PTEN

Yang, Yongkang; Xi, Wen‐Yan; Xi, Ruxing; Li, Jing‐Yuan; Qin, Li; Gao, Yue

doi:10.3892/or.2015.3821

Cited by 53 publications

(39 citation statements)

References 44 publications

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“…Contrary to our finding of it being suppressed in BCC relative to SCC and HK, miR-494 is induced in several cancers (malignant breast cancer, esophageal squamous cell carcinoma, gastric carcinoma, oral cancer, ovarian cancer and pancreatic cancer) (148)(149)(150)(151)(152)(153)(154)(155)(156)(157). Rather than inferring its suppression in BCC, perhaps it is induced early in the transformation of keratinocytes to SCC.…”

Section: A Study Population Demographics and Histopathological Analysiscontrasting

confidence: 99%

MiRNA expression changes in arsenic-induced skin cancer in vitro and in vivo.

Al-Eryani¹

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Section: A Study Population Demographics and Histopathological Analysiscontrasting

confidence: 99%

MiRNA expression changes in arsenic-induced skin cancer in vitro and in vivo.

Al-Eryani¹

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“…Previous studies have identified that miR-494 is associated with the development of tumors (8,9). However, there are a limited number of studies on the expression and the function of miR-494 in cervical cancer.…”

Section: Mir-494 Expression Is Associated With Clinicopathological Fementioning

confidence: 99%

“…Previous studies have revealed abnormal expression of a number of miRNAs in cervical cancer, including miR-214, miR-21, miR-143 and miR-145 (4-7). In cervical cancer, miR-214 expression is decreased, which has been reported to inhibit cancer progression through targeting mitochondrial transcription factor A (8). miR-21 overexpression may inhibit the expression of phosphatase and tensin homolog (PTEN), and promote cancer cell proliferation and migration (9).…”

Section: Introductionmentioning

confidence: 99%

“…A previous study demonstrated that the expression level of miR-145 was significantly decreased in human cervical cancer miR-494 inhibits cervical cancer cell proliferation through upregulation of SOCS6 expression LEI CHENG 1,2 , BEIHUA KONG 1 , YING ZHAO 2 and JIE JIANG tissues when compared with corresponding adjacent normal tissues and associated with tumor progression, and poor prognosis (11). Furthermore, miR-494 serves as a tumor suppressor miRNA and abnormal expression of miR-494 has been identified in a number of tumor tissue types (8,9). However, whether the expression level of miR-494 is altered in cervical cancer remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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miR-494 inhibits cervical cancer cell proliferation through upregulation of SOCS6 expression

et al. 2017

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Abstract. It is unclear how microRNA (miR)-494 inhibits the proliferation of cervical cancer cells by altering the expression of SOCS6. Therefore, the present study aimed to investigate the molecular mechanism underlying miR-494 regulation of suppressor of cytokine signaling 6 (SOCS6) in human cervical cancer samples and the human cervical cancer HeLa cell line. The expression of miR-494 was determined using reverse transcription-quantitative polymerase chain reaction. In addition, TargetScan was used to predict miR-494 target genes and the luciferase reporter assay was used to determine whether SOCS6 was a direct target of miR-494. The results of the present study demonstrated that compared with the cervical intraepithelial neoplasia and normal cervical tissues, the miR-494 expression level in cervical cancer samples was significantly decreased (P<0.01). In addition, compared with normal cervical tissue, miR-494 expression level was significantly decreased in cervical intraepithelial lesions (P<0.05). Furthermore, the expression of miR-494 was associated with patients with or without lymph node metastasis, clinical stage and depth of stromal invasion (P<0.01); however, miR-494 expression was not identified to be associated with age, tumor size and menopausal status (P>0.05). Transfection of a miR-494 mimic significantly increased the expression level of miR-494 in HeLa cells (P<0.01), and anti-miR-494 transfection decreased the expression of miR-494 (P<0.01). An MTT proliferation assay and Boyden chamber invasion ability assay revealed that miR-494 mimic transfection significantly inhibited the proliferation, and invasion ability of HeLa cells (P<0.01), whereas anti-miR-494 transfection significantly increased the proliferation and invasion ability (P<0.05). SOCS6 was predicted, using bioinformatics, to be the target gene of miR-494 and this was validated using a luciferase reporter assay. Western blot analysis revealed that transfection of miR-494 significantly increased the expression of SOCS6 in HeLa cells, and transfection of anti-miR-494 significantly decreased the expression of SOCS6. Therefore, the results of the present study demonstrated that miR-494 expression in cervical cancer was significantly decreased. Exhibiting a decreased expression level of miR-494 may result in enhanced proliferative and invasive abilities of HeLa cell, thus contributing to the occurrence, and development of cervical cancer. IntroductionCervical cancer is the second most common type of female malignancy, subsequent to breast cancer. There were an estimated 527,600 new cervical cancer cases and 265,700 deaths worldwide in 2012 (1). Although cervical cytology screening has enabled early detection and early treatment of cervical cancer, the incidence and mortality rates continue to increase each year (2,3). The majority of patients with cervical cancer are treated with standard radiation therapy and chemotherapy, but therapeutic response varies. Thus, studies are required to determine the pathogenesis of cervical cancer in orde...

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“…Numerous studies have been performed on the role and related mechanism of miRNAs in numerous different kinds of diseases (9)(10)(11)(12). miRNAs bind primarily to the 3'-untranslated region (3'UTR) of their target messenger RNAs (mRNAs) to reduce their stability and decrease the expression of target mRNAs at the post-transcriptional level (13), which play important roles in various biological processes including cell growth, proliferation, differentiation and death (14)(15)(16)(17)(18)(19). Concerning chemotherapy in various types of cancers such as breast cancer, lung adenocarcinoma, glioblastoma, and ovarian cancer, recent studies have shown that miRNAs also act in important roles (20)(21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

miR-1291 targets mucin 1 inhibiting cell proliferation and invasion to promote cell apoptosis in esophageal squamous cell carcinoma

et al. 2015

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Abstract. MicroRNAs (miRNAs) are well known as important regulators in various cancer development. In the present study, we focused on the expression and biological function of miR-1291 in esophageal squamous cell carcinoma (ESCC). Compared with adjacent non-tumorous tissue samples, qRT-PCR data showed significant downregulation of miR-1291 in 54 ESCC tissue samples (P<0.05), which was also significantly associated with lymph node metastases and clinical stage (P<0.05). Cell Counting Kit-8 (CCK-8), colony formation, Transwell and flow cytometric apoptosis assays were performed to detect the effect of miR-1291 upregulation, and the results showed inhibition of the proliferation, invasion and promotion of apoptosis in EC9706 and EC-1 cells. Using bioinformatic analyses, we found that mucin 1 (MUC1) was a potential target for miR-1291. Luciferase assays were performed to reveal that miR-1291 inhibited MUC1 expression by targeting the seed region of MUC1 3'-untranslated region (3'UTR). We also found that the expression of MUC1 lacking in 3'UTR abrogated the anti-invasion and pro-apoptosis function of miR-1291. Our results demonstrated the importance of miR-1291 in targeting MUC1 for the regulation of esophagus cancer growth, invasion and apoptosis, and may be helpful for developing new targets for early diagnosis or new therapeutic targets for ESCC.

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MicroRNA-494 promotes cervical cancer proliferation through the regulation of PTEN

Cited by 53 publications

References 44 publications

MiRNA expression changes in arsenic-induced skin cancer in vitro and in vivo.

MiRNA expression changes in arsenic-induced skin cancer in vitro and in vivo.

miR-494 inhibits cervical cancer cell proliferation through upregulation of SOCS6 expression

miR-1291 targets mucin 1 inhibiting cell proliferation and invasion to promote cell apoptosis in esophageal squamous cell carcinoma

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