MicroRNA-485-5p represses melanoma cell invasion and proliferation by suppressing Frizzled7

Wu, Jiawen; Li, Jing; Ren, Jizhong; Zhang, Dingwei

doi:10.1016/j.biopha.2017.03.064

Cited by 41 publications

(37 citation statements)

References 48 publications

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“…Lower miR-485 levels were correlated with a high incidence of metastatic events and high prostate-specific antigen (PSA) levels; similar trends were observed for lymph node invasion and Gleason score (41). The downregulation of miR-485 was also observed in ovarian (23), bladder (24), breast (25,26), melanoma (42) and oral tongue squamous cell carcinoma (43). These findings suggest that miR-485 may serve as a diagnostic and prognostic biomarker for these specific tumour types.…”

Section: Discussionmentioning

confidence: 65%

“…Previously, a number of miR-485 targets have been validated in a number of types of cancer, including Flot2 (37) in lung adenocarcinoma, stanniocalcin 2 (39) and EMMPRIN (40) in hepatocellular carcinoma, HMAG2 (24) in bladder cancer, T47D (25) and PGC-1α (26) in breast cancer and Frizzled7 (42) in melanoma. In this study, GAB2 was identified as a novel direct target of miR-485 in CRC.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA-485 plays tumour-suppressive roles in colorectal cancer by directly targeting GAB2

Yan

et al. 2018

Oncol Rep

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Colorectal cancer (CRC) is reported to be the third most common cancer and the fourth leading cause of cancer-related deaths around the world. MicroRNA-485 (miR-485) has been reported to be aberrantly expressed and play important roles in several types of human malignancy. However, the expression level, biological functions and underlying molecular mechanisms of miR-485 in CRC remain unclear. Therefore, the aim of the present study was to determine miR-485 expression levels and their clinical significance in CRC and to explore the functions and underlying mechanisms of miR-485 in this disease. In the present study, miR-485 was lowly expressed in CRC tissues and cell lines. Decreased miR-485 expression was associated with tumour size, lymph node metastasis, distant metastasis and TNM stage. Functional assays indicated that upregulation of miR-485 impaired CRC cell proliferation, invasion and induced cell apoptosis. Grb2-associated binding 2 (GAB2) was identified as a direct target of miR-485 in CRC. GAB2 was upregulated in CRC tissues and was negatively correlated with the miR-485 expression level. Furthermore, GAB2 knockdown simulated the tumour-suppressing roles of miR-485 overexpression in CRC cells. Moreover, restored GAB2 expression reversed the effects of miR-485 overexpression in CRC cells. In addition, miR-485 suppressed the AKT and ERK signalling pathways in CRC by directly targeting GAB2. Collectively, these findings demonstrate that miR-485 may play tumour suppressive roles in CRC by directly targeting GAB2 and indirectly regulating AKT and ERK signalling pathways, suggesting that miR-485 may be a potential therapeutic target for patients with this disease.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

MicroRNA-485 plays tumour-suppressive roles in colorectal cancer by directly targeting GAB2

Yan

et al. 2018

Oncol Rep

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“…In hepatocellular carcinoma, miR-485 expression is downregulated in tumor tissues and cell lines, and is associated with tumor size, TNM stage, metastasis and tumor number (33,34). miR-485 expression is also downregulated in bladder cancer (35), breast cancer (36,37), melanoma (38) and oral-tongue squamous cell carcinoma (39). These findings suggest that miR-485 is frequently downregulated in human malignancies and is therefore a potential biomarker for the detection and prognosis of specific types of human cancer.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑485 targets MACC1 and inhibits cervical cancer cell proliferation and invasion

et al. 2018

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A large body of evidence has indicated that microRNAs (miRNAs/miRs) have essential roles in the development and progression of cervical cancer. Thus, miRNAs with dysregulated expression are potential biomarkers for cervical cancer diagnosis and prognosis. In the present study, expression levels of miR‑485 were detected in cervical cancer tissues and cell lines. The effects of miR‑485 overexpression on the proliferation and invasion of cervical cancer cells were determined with Cell Counting kit‑8 and Transwell invasion assays. The mechanisms underlying the action of miR‑485 in cervical cancer were investigated using bioinformatics analysis, a luciferase reporter assay, reverse transcription‑quantitative polymerase chain reaction and western blot analysis. In addition, the association between miR‑485 and metastasis associated in colon cancer‑1 (MACC1) in cervical cancer tissues was examined. The present study demonstrated that miR‑485 expression was significantly downregulated in cervical cancer tissues and cell lines. Reduced miR‑485 expression in patients with cervical cancer was correlated with International Federation of Gynecology and Obstetrics stage and lymph node metastasis. Furthermore, restored expression of miR‑485 significantly reduced cervical cancer cell proliferation and invasion. MACC1 was identified as a direct target gene of miR‑485 in cervical cancer. MACC1 expression was significantly upregulated in cervical cancer specimens and was inversely correlated with miR‑485 expression. Additionally, the restored expression of MACC1 eliminated the suppressive effects of miR‑485 overexpression on the proliferation and invasion of cervical cancer cells. Notably, the upregulation of miR‑485 suppressed the MET proto‑oncogene, receptor tyrosine kinase (Met)/RAC‑α serine/threonine‑protein kinase (AKT) signaling pathway. These results demonstrated that miR‑485 may perform its tumor suppressive function in cervical cancer by directly targeting MACC1 and inhibiting the Met/AKT signaling pathway. Therefore, the miR‑485/MACC1 axis may be a novel and effective therapeutic target in cervical cancer.

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“…Abnormally expressed miRNAs play an important role in melanoma formation and progression by serving as potential biomarkers and therapeutic targets (35)(36)(37). In the present study, we investigated the expression levels, the exact roles and the regulatory mechanism of miR-326 in melanoma.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-326 inhibits melanoma progression by targeting KRAS and suppressing the AKT and ERK signalling pathways

Kang

Zhang

et al. 2017

Oncol Rep

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Abstract. Melanoma is the seventh most common malignancy in females and the fifth most common cancer in males worldwide. An increasing number of studies have reported that microRNA (miRNA) dysregulation is frequently observed in various types of human cancers, including melanoma. Abnormally expressed miRNAs play an important role in melanoma formation and progression by serving as potential biomarkers and therapeutic targets. Recently, miRNA-326 (miR-326) has been reported to be differentially expressed in various types of tissues and play important roles in tumourigenesis and tumour development. However, the expression levels, biological roles and underlying mechanisms of miR-326 in melanoma remain unknown. In the present study, we demonstrated that miR-326 was significantly downregulated in melanoma tissues and cell lines. Functional assays revealed that the enforced expression of miR-326 suppressed melanoma cell proliferation and invasion and increased cell apoptosis in vitro. Using bioinformatic analysis, Kirsten rat sarcoma viral oncogene homolog (KRAS) was predicted as a potential target of miR-326. Luciferase reporter assay confirmed that miR-326 could directly target the 3'-untranslated region of KRAS. In addition, reverse transcription-quantitative polymerase chain reaction and western blot analysis revealed that miR-326 upregulation decreased the KRAS expression in melanoma cells at both the mRNA and protein level. Furthermore, KRAS was upregulated in melanoma tissues and inversely correlated with miR-326 expression. In addition, the KRAS knockdown phenocopied the tumour-suppressing effects of miR-326 overexpression on melanoma cells. The restoration of the expression of KRAS markedly reversed the antitumour effects induced by miR-326 overexpression in melanoma cells. Further experiments indicated that miR-326 inactivated the AKT and ERK signalling pathways in melanoma. Collectively, these results revealed that miR-326 serves as a tumour suppressor in melanoma by targeting KRAS and regulating the AKT and ERK signalling pathways, indicating that miR-326 may be a promising therapeutic target for melanoma patients.

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MicroRNA-485-5p represses melanoma cell invasion and proliferation by suppressing Frizzled7

Cited by 41 publications

References 48 publications

MicroRNA-485 plays tumour-suppressive roles in colorectal cancer by directly targeting GAB2

MicroRNA-485 plays tumour-suppressive roles in colorectal cancer by directly targeting GAB2

MicroRNA‑485 targets MACC1 and inhibits cervical cancer cell proliferation and invasion

MicroRNA-326 inhibits melanoma progression by targeting KRAS and suppressing the AKT and ERK signalling pathways

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