microRNA-425-5p is upregulated in human gastric cancer and contributes to invasion and metastasis in vitro and in vivo

Abstract

Emerging evidence indicates that microRNAs are involved in the development and progression of gastric cancer (GC), functioning as tumor suppressors or oncogenes. The aim of the current study was to explore the potential mechanism of microRNA-425-5p (miR-425-5p) in GC. Using reverse transcription quantitative polymerase chain reaction, miR-425-5p expression was detected in GC cell lines (SGC-7901, MKN-28, MKN-45, BGC-823 and AGS) and the GES-1 cell line. Cell proliferation, soft agar colony formation, flow cytometry, haptotactic migration and matrigel chemoinvasion assays were used to test the proliferation, apoptosis, invasion and migration of BGC-823 cells transfected with miR-425-5p mimics or an inhibitor. Female BALB/c mice were randomly divided into three groups. Each experimental group contained five mice. BGC 823 cells were stably transfected with miR-425-5p inhibitor, wild type or negative control and were injected into the mice through the tail vein. It was found that miR-425-5p expression was significantly upregulated in all the GC cell lines when compared with the GES-1 cell line. Downregulation of miR-425-5p expression inhibited GC cell proliferation, invasion and migration. In the studies, downregulation of miR-425-5p expression suppressed pulmonary metastasis in the nude mice. Thus, miR-425-5p was demonstrated to have the potential to become a novel metastasis-associated gene; however, the mechanisms associated with these effects require further study. In the future, the development of miR-425-5p as a novel therapeutic strategy for the treatment of GC may be possible.

A panel of microRNA signature in serum for colorectal cancer diagnosis

“…Zhang et al showed that miR-425-5p could regulate chemoresistance in CRC cells via regulating Programmed Cell Death 10 [33]. In addition, miR-425-5p has been reported to promote tumorigenicity and aggressiveness in breast cancer and gastric cancer [31, 34]. miR-425-5p was discovered for the first time to be valuable biomarker of CRC in our study.…”

Pre-Exposure Gene Expression in Baboons with and without Pancytopenia after Radiation Exposure

“…We found that miR-425-5p appeared promising with respect to normal tissue response to radiation in our examination of healthy baboons. Accordingly, this miRNA has been previously associated with chemo- and radiosensitivity [20,21], cell proliferation [22,23], and cell death [20]. …”

Deep sequencing reveals microRNAs predictive of antiangiogenic drug response

“…Among these 2 miRNAs, miR-NA-425 has been associated with tumor stage in gastric (32) and lung cancer (33), and in RCC, it has been suggested as being a potential ccRCC biomarker (34) associated with poor prognosis for chromophobe RCC (35) and decreased PFS during sunitinib treatment (17). miR-1307 plays a role in chemoresistance in ovarian cancer (36), and it has been suggested to contribute to colorectal carcinogenesis (37).…”