MicroRNA-421 inhibits breast cancer metastasis by targeting metastasis associated 1

Pan, Yongqin; Jiao, Genlong; Wang, Cunchuan; Yang, Jingge; Yang, Wah

doi:10.1016/j.biopha.2016.08.058

Cited by 41 publications

(33 citation statements)

References 29 publications

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“…Cell migration and invasion were evaluated by conducting the Transwell assay, according to a previously described method 19,20 . Transwell inserts (pore size, 8 μm; BD Biosciences, San Jose, CA, USA) were precoated with or without Matrigel (BD Biosciences) for use in the migration and invasion assays.…”

Section: Transwell Assaymentioning

confidence: 99%

LncRNA AC093818.1 accelerates gastric cancer metastasis by epigenetically promoting PDK1 expression

Long

et al. 2020

Cell Death Dis

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Gastric cancer (GC) is a highly prevalent type of metastatic tumor. The mechanisms underlying GC metastasis are poorly understood. Some long noncoding RNAs (lncRNAs) reportedly play key roles in regulating metastasis of GC. However, the biological roles of five natural antisense lncRNAs (AC093818.1, CTD-2541M15.1, BC047644, RP11-597M12.1, and RP11-40A13.1) in GC metastasis remain unclear. In this study, the expression of these lncRNAs was measured by quantitative reverse transcription-polymerase chain reaction. Migration and invasion were evaluated by wound-healing and the Transwell assay, respectively. Stable cells were injected into the tail veins of nude mice. Sections of collected lung and liver tissues were stained using hematoxylin and eosin. Protein expression was analyzed by western blot. RNA immunoprecipitation (RIP) assay was used to verify whether the STAT3 and SP1 transcription factors bound to AC093818.1 in GC cells. Expression levels of the five lncRNAs, especially AC093818.1, were significantly upregulated in metastatic GC tissues relative to those in nonmetastatic GC tissues. AC093818.1 expression was correlated with invasion, lymphatic metastasis, distal metastasis, and tumor-node-metastasis stage. AC093818.1 expression was highly sensitive and specific in the diagnosis of metastatic or nonmetastatic GC. AC093818.1 overexpression promoted GC migration and invasion in vitro and in vivo. AC093818.1 overexpression increased PDK1, p-AKT1, and p-mTOR expression levels. AC093818.1 silencing decreased these expressions. AC093818.1 bound to transcription factors STAT3 and SP1, and SP1 or STAT3 silencing could alleviated the effect of AC093818.1 overexpression. The data demonstrate that lncRNA AC093818.1 accelerates gastric cancer metastasis by epigenetically promoting PDK1 expression. LncRNA AC093818.1 may be a potential therapeutic target for metastatic GC.

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Section: Transwell Assaymentioning

confidence: 99%

LncRNA AC093818.1 accelerates gastric cancer metastasis by epigenetically promoting PDK1 expression

Long

et al. 2020

Cell Death Dis

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“…Total RNA was extracted from tissues using the Trizol reagent (Invitrogen, Carlsbad, CA). The miR-421 expression level was determined by quantitative real-time RT-PCR using Taqman assay kits (Applied Biosystems, Foster City, CA), with the forward primer as 5'-ATCAACAGACAT-TAATTGGGCGC-3' [12]. U6 small nuclear RNA was used as internal normalized reference, with the primers as 5'-CGCTTCGGCAGCACATATACTAA-3' (forward) and 5'-TATGGAACGCTTCACGAATTTGC-3' (reverse).…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-421 inhibits caspase-10 expression and promotes breast cancer progression

Tb¹,

Hs²,

Mq³

et al. 2018

neo

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Breast cancer is one of the most prevalent and fatal diseases around the world. The mechanism of tumorigenesis in breast cancer remains to be clarified. miR-421 plays an oncogenic role in many cancers. Although, the clinical significance of miR-421 in patients with breast cancer is still to be investigated. Caspase-10 is one of the initiator of apoptosis. But the relationship between miR-421 and caspase-10 has not been investigated. In the present study, we found that miR-421 was expressed much higher in breast cancer tissues compared to those in adjacent non-tumor tissues. Furthermore, miR-421 promotes cell proliferation and colony formation in vitro. miR-421 inhibits cell apoptosis probably through restraining caspase-10 expression. Thus, miR-421 might be a potential diagnostic maker and therapeutic target for breast cancer.

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“…miR-421 is down-regulated in BC and down-regulation correlates with lymph node metastasis, recurrence and metastasis (156). miR-421 targets metastasis-associated protein-1 (MTA-1), which is involved in chromatin remodeling (157).…”

Section: Further Breast Cancer Metastasis-suppressive Mirsmentioning

confidence: 99%

“…miR-421 targets metastasis-associated protein-1 (MTA-1), which is involved in chromatin remodeling (157). miR-421 knock-down leads to invasion and metastasis of MCF-7 cells and overexpression in MDA-MB-231 cells suppresses invasion and metastasis (156). (158).…”

Section: Further Breast Cancer Metastasis-suppressive Mirsmentioning

confidence: 99%

The Role of micro RNAs in Breast Cancer Metastasis: Preclinical Validation and Potential Therapeutic Targets

Weidle

Dickopf

Hintermair

et al. 2018

CGP

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MicroRNA-421 inhibits breast cancer metastasis by targeting metastasis associated 1

Cited by 41 publications

References 29 publications

LncRNA AC093818.1 accelerates gastric cancer metastasis by epigenetically promoting PDK1 expression

LncRNA AC093818.1 accelerates gastric cancer metastasis by epigenetically promoting PDK1 expression

MicroRNA-421 inhibits caspase-10 expression and promotes breast cancer progression

The Role of micro RNAs in Breast Cancer Metastasis: Preclinical Validation and Potential Therapeutic Targets

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