MicroRNA-383: A tumor suppressor miRNA in human cancer

Jafarzadeh, Abdollah; Noori, Majid; Sarrafzadeh, Shaghayegh; Zadeh, Seyed Saeed Tamehri; Nemati, Maryam; Chatrabnous, Nazanin; Jafarzadeh, Sara; Hamblin, Michael R.; Abadi, Mohammad Hassan Jafari Najaf; Mirzaei, Hamed

doi:10.3389/fcell.2022.955486

Cited by 22 publications

(5 citation statements)

References 142 publications

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“…In our study, we utilized a dual-luciferase reporter assay and FISH analysis to con rm the binding of miR-383-5p to circ_0007386. Our ndings align with previous reports on miR-383-5p (39), demonstrating its role in promoting apoptosis and inhibiting proliferation in NSCLC. The rescue experiments further corroborated circ_0007386's role as a sponge for miR-383-5p NSCLC progression.…”

Section: Discussionsupporting

confidence: 92%

Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression

Li,

Liu,

Chen

et al. 2024

Preprint

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Background The progression of non-small cell lung cancer (NSCLC) is significantly influenced by circular RNAs (circRNAs), especially in tumor hypoxia microenvironment. However, the precise functions and underlying mechanisms of dysregulated circRNAs in NSCLC remain largely unexplored. Methods Differentially expressed circRNAs in NSCLC tissues were identified through high-throughput RNA sequencing. The characteristics of circ_0007386 were rigorously confirmed via Sanger sequencing, RNase R treatment and actinomycin D treatment. The effects of circ_0007386 on proliferation and apoptosis were investigated using CCK8, cloning formation assays, TUNEL staining, and flow cytometry assays in vitro. In vivo, xenograft tumor models were used to evaluate its impact on proliferation. Mechanistically, the regulatory relationships of circ_0007386, miR-383-5p and CIRBP were examined through dual luciferase reporter assays and rescue experiments. Additionally, we detected the binding of EIF4A3 to CRIM1 pre-mRNA by RNA immunoprecipitation and the interaction between YAP1 and EIF4A3 under hypoxic conditions by co-immunoprecipitation. Results Our investigation revealed a novel circRNA, designated as circ_0007386, that was upregulated in NSCLC tissues and cell lines. Circ_0007386 modulated proliferation and apoptosis in NSCLC both in vitro and in vivo. Functionally, circ_0007386 acted as a sponge for miR-383-5p, targeting CIRBP, which influenced NSCLC cell proliferation and apoptosis via the PI3K/AKT signaling pathway. Furthermore, under hypoxic conditions, the interaction between YAP1 and EIF4A3 was enhanced, leading to the displacement of EIF4A4 from binding to CRIM1 pre-mRNA. This facilitated the back-splicing of CRIM1 pre-mRNA, increasing the formation of circ_0007386. The circ_0007386/miR-383-5p/CIRBP axis was significantly associated with the clinical features and prognosis of NSCLC patients. Conclusions Circ_0007386, regulated by YAP1-EIF4A3 interaction under hypoxia conditions, plays an oncogenic role in NSCLC progression via the miR-383-5p/CIRBP axis.

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Section: Discussionsupporting

confidence: 92%

Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression

Li,

Liu,

Chen

et al. 2024

Preprint

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“…Similarly, miR-7-5p, also downregulated in colorectal cancer tissues and cell lines, inhibits cell proliferation and migration by targeting Krüppel-like factor 4 (KLF4) [23]. Furthermore, miR-383-3p, which is downregulated in colorectal cancer tissues, functions as a tumor suppressor by targeting pro-tumor genes and is associated with tumor size, metastasis, and tumor stage [24]. The concurrent upregulation of lncRNA EVADR and the downregulation of these miRNAs in colorectal cancer suggests that EVADR may play a crucial role in the progression of the disease, potentially through its interactions with these miRNAs.…”

Section: Discussionmentioning

confidence: 99%

The expression of lncRNAs EVADR and LUESCC in Colorectal tumor tissues and their association with the CRC risk in an Iranian population

Ahmadzadeh,

shahamiri,

Raeisi

et al. 2024

Preprint

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Colorectal cancer (CRC) is a prevalent form of cancer that impacts individuals around the world. It is the second most common cause of cancer-related deaths worldwide. Long non-coding RNAs (lncRNAs) are a type of regulatory RNA that can impact gene expression. EVADR and LUESCC are tow novel lncRNA specifically expressed in tumors of glandular origin, such as colon.in this study we aimed to investigate the expression of EVADR and LUESCC in Colorectal tumor tissues in Iranian patients. We selected 50 cases of colorectal tumor tissues (FFPE) from individuals with sporadic CRC who were referred from the Pathology Department of Imam Hossein hospital in Tehran, Iran. The study results offer valuable insights into the expression patterns of LUESCC and EVADR lncRNAs in CRC patients. The findings show a significant upregulation of both LUESCC and EVADR lncRNAs in CRC patients compared to normal tissues, with notable fold-change values and statistical significance. The ROC curve analysis further emphasizes the potential diagnostic value of these lncRNAs. Additionally, the differential expression of LUESCC and EVADR lncRNAs in different differentiation groups suggests a potential association between these lncRNAs and CRC tumor grade. The significant increase in expression observed in well, poor, and intermediate differentiation groups compared to normal tissues underscores the potential role of these lncRNAs in CRC progression and aggressiveness. The present study also revealed distinct expression patterns of LUESCC and EVADR lncRNAs in relation to metastasis and fecal occult blood. Overall, these findings shed light on the diagnostic and prognostic potential of LUESCC and EVADR lncRNAs in CRC.

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“…Increasing evidence has shown that miRNAs are important regulators of various cellular processes. In recent years, it has been widely studied in the occurrence and development of EC[ 48 - 51 ]. In this study, we used the Target Scan database to predict that mi-495-3p has a specific binding site in BUB1 mRNA 3′-UTR.…”

Section: Discussionmentioning

confidence: 99%

Baitouweng decoction suppresses growth of esophageal carcinoma cells through miR-495-3p/BUB1/STAT3 axis

Yang,

Chen,

Song

et al. 2024

World J Gastrointest Oncol

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BACKGROUND Esophageal carcinoma (EC) is one of the most prevalent cancers in human populations worldwide. Baitouweng decoction is one of the most important Chinese medicine formulas, with the potential to treat cancer. AIM To investigate the role and mechanism of Baitouweng decoction on EC cells. METHODS Differentially expressed genes (DEGs) in EC tissues and normal tissues were screened by the cDNA microarray technique and by bioinformatics methods. The target genes of microRNAs were predicted based on the TargetScan database and verified by dual luciferase gene reporter assay. We used Baitouweng decoction to intervene EC cells, and detected the activity of EC9706 and KYSE150 cells by the MTT method. Cell cycle and apoptosis were measured by flow cytometry. The expression of BUB1 mRNA and miR-495-3p was measured by qRT-PCR. The protein levels of BUB1, STAT3, p-STAT3, CCNB1, CDK1, Bax, Caspase3, and Caspase9 were measured by Western blot analysis. The migration and invasion abilities of the cells were measured by wound-healing assay and Transwell invasion assay, respectively. RESULTS DEGs identified are involved in biological processes, signaling pathways, and network construction, which are mainly related to mitosis. BUB1 was the key hub gene, and it is also a target gene of miR-495-3p. Baitouweng decoction could upregulate miR-495-3p and inhibit BUB1 expression. In vitro experiments showed that Baitouweng decoction significantly inhibited the migration and invasion of EC cells and induced apoptosis and G2/M phase arrest. After treatment with Baitouweng decoction, the expression of Bax, Caspase 3, and Caspase 9 in EC cells increased significantly, while the expression of BUB1, CCNB1, and CDK1 decreased significantly. Moreover, the STAT3 signaling pathway may play an important role in this process. CONCLUSION Baitouweng decoction has a significant inhibitory effect on EC cell growth. BUB1 is a potential therapeutic target for EC. Further analysis showed that Baitouweng decoction may inhibit the growth of EC cells by upregulating miR-495-3p targeting the BUB1-mediated STAT3 signal pathway.

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MicroRNA-383: A tumor suppressor miRNA in human cancer

Cited by 22 publications

References 142 publications

Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression

Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression

The expression of lncRNAs EVADR and LUESCC in Colorectal tumor tissues and their association with the CRC risk in an Iranian population

Baitouweng decoction suppresses growth of esophageal carcinoma cells through miR-495-3p/BUB1/STAT3 axis

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