MicroRNA-382-5p aggravates breast cancer progression by regulating the RERG/Ras/ERK signaling axis

Ho, Jar-Yi; Hsu, Ren Jun; Liu, Jui Ming; Chen, Szu Chi; Liao, Guo Shiou; Gao, Hong; Yu, Cheng

doi:10.18632/oncotarget.12338

Cited by 66 publications

(65 citation statements)

References 46 publications

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“…Therefore, we decided to validate miR analysis by RT‐qPCR using the GLP standards. We analyzed miR‐146a‐5p, miR‐155‐5p, miR‐382‐5p, and miR‐451a, which have been described as potential blood‐based biomarkers for various diseases . For the validation of miR analysis we considered methodological differences in RNA extraction and isolation.…”

Section: Discussionmentioning

confidence: 99%

“…We analyzed miR-146a-5p, miR-155-5p, miR-382-5p, and miR-451a, which have been described as potential blood-based biomarkers for various diseases. [21][22][23][24] For the validation of miR analysis we considered methodological differences in RNA extraction and isolation. Here, we compared a simple and cost-efficient phenol/GTC method according to Chomczynski & Sacci (1987) 13 and a columnbased RNA extraction.…”

Section: Discussionmentioning

confidence: 99%

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Validation of extracellular miRNA quantification in blood samples using RT‐qPCR

Fauth

Hegewald

Schmitz³

et al. 2019

FASEB BioAdvances

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Extracellular microRNAs (miRs) have been proposed as important blood‐based biomarkers for several diseases. Contrary to proteins and other RNA classes, miRs are stable and easily detectable in body fluids. In this respect, miRs represent a perfect candidate for minimal invasive biomarkers which can hopefully become a complement for invasive histological examinations of tumor tissue. Despite the high number of miR biomarker studies, the specificity and reproducibility of these studies is missing. Therefore, the standardization of pre‐analytical and analytical methods is urgently needed. Here, we validated miR analysis for RNA isolation and miR quantification by quantitative polymerase chain reaction (RT‐qPCR) based on good laboratory practice (GLP). Validation was carried out exemplarily on four miRs, which had already been described as potential biomarkers in previous studies. As basis for RNA analysis using RT‐qPCR, the Minimum Information for Publication of Quantitative Real‐Time PCR Experiments were applied and adapted on the analysis of circulating miRs from human plasma. In our study, we identified and solved several pitfalls from handling to normalization strategy in the analysis of extracellular miRs that lead to inconsistent and non‐repeatable data. Principles of GLP set a framework of experimental design, performance and monitoring to ensure high quality and reliable data. Within this study, we appointed first acceptance criteria for circulating miR quantification during validation which set standards for future miR quantification in blood samples.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Validation of extracellular miRNA quantification in blood samples using RT‐qPCR

Fauth

Hegewald

Schmitz³

et al. 2019

FASEB BioAdvances

View full text Add to dashboard Cite

show abstract

“…The molecular mechanisms of Tec treatment on breast cancer cells were explored. Breast cancer cells often express continued active survival and signaling pathways, such as AKT and MAPK signaling, as well as gene mutation, rearrangements and chromosomal translocation in other signaling pathways (31)(32)(33). The survival-signaling pathway serves an important role in proliferation, tumorigenesis, anti-apoptosis and drug resistance (34,35).…”

Section: Discussionmentioning

confidence: 99%

Suppression of human breast cancer cells by tectorigenin through downregulation of matrix metalloproteinases and MAPK signaling inï¿½vitro

Zeng¹,

Yuan²,

Shen³

et al. 2017

Mol Med Report

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Breast cancer is a major life‑threatening malignancy and is the second highest cause of mortality. The aim of the present study was to investigate the effects of tectorigenin (Tec), a Traditional Chinese Medicine, against human breast cancer cells in vitro. MDA‑MB‑231 and MCF‑7 human breast cancer cells were treated with various concentrations of Tec. Cell proliferation was evaluated using the Cell Counting kit‑8 assay, and apoptosis and the cell cycle were examined by flow cytometry. The migratory and invasive abilities of these cells were detected by Transwell and Matrigel assays, respectively. Metastasis‑, apoptosis‑ and survival‑related gene expression levels were measured by reverse transcription‑quantitative polymerase chain reaction and western blotting. The results indicated that Tec was able to inhibit the proliferation of MDA‑MB‑231 and MCF‑7 cells in a dose‑ and time‑dependent manner. Furthermore, Tec treatment induced apoptosis and G0/G1‑phase arrest, and inhibited cell migration and invasion. Tec treatment decreased the expression of matrix metalloproteinase (MMP)‑2, MMP9, BCL‑2, phosphorylated‑AKT and components of the mitogen‑activated protein kinase (MAPK) signaling pathway, and increased the expression of BCL‑2‑associated X, cleaved poly [ADP‑ribose] polymerase and cleaved caspase‑3. In conclusion, Tec treatment suppressed human breast cancer cells through the downregulation of AKT and MAPK signaling and the upregulated expression and/or activity of the caspase family in vitro. Therefore, Tec may be a potential therapeutic drug to treat human breast cancer.

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“…On the one hand, oestrogen is known as a tumour promoter through its activation of the RAS/ERK pathway. 58,59 In vitro treatment of MD-MBA-231…”

Section: Breast Cancermentioning

confidence: 99%

Calcium channel blockers and the incidence of breast and prostate cancer: A meta-analysis

et al. 2018

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Although a statistically significant association between CCB use and incidence of BCa does exist, the limitations of the individual studies restrict the clinical application of this relationship. Our meta-regression model does newly identify a 9-year latency period of CCB use and a significantly increased risk of BCa. No significant association exists between CCB use and the incidence of PCa. Our meta-regression shows CCB may have a protective effect upon PCa incidence among older populations.

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MicroRNA-382-5p aggravates breast cancer progression by regulating the RERG/Ras/ERK signaling axis

Cited by 66 publications

References 46 publications

Validation of extracellular miRNA quantification in blood samples using RT‐qPCR

Validation of extracellular miRNA quantification in blood samples using RT‐qPCR

Suppression of human breast cancer cells by tectorigenin through downregulation of matrix metalloproteinases and MAPK signaling inï¿½vitro

Calcium channel blockers and the incidence of breast and prostate cancer: A meta-analysis

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