MicroRNA-379 inhibits the proliferation, migration and invasion of human osteosarcoma cells by targetting EIF4G2

Abstract

Osteosarcoma (OS) is an aggressive malignant mesenchymal neoplasm amongst adolescents. The aim of the present study was to explore the various modes of action that miR-379 has on the proliferation, migration, and invasion of human OS cells. miR-379 achieves this by targetting eukaryotic initiation factor 4GII (EIF4G2). Human OS cell lines U2OS and MG-63 were selected and assigned into blank, miR-379 mimics, miR-379 mimic negative control (NC), miR-379 inhibitors, miR-379 inhibitor NC, EIF4G2 shRNA, control shRNA, and miR-379 inhibitor + EIF4G2 shRNA group. The miR-379 expression and EIF4G2 mRNA expression were detected utilising quantitative real-time PCR (qRT-PCR) and the EIF4G2 protein expression using Western blotting. MTT assay, scratch test, Transwell assay, and flow cytometry were performed to determine the proliferation, migration, invasion, and cell cycle, respectively. In comparison with the miR-379 mimic NC group, the miR-379 mimics group had decreased EIF4G2 expression; the miR-379 inhibitors group indicated an increased EIF4G2 expression. Compared with the control shRNA group, the EIF4G2 expression was lower in the EIF4G2 shRNA group and the miR-379 expression was dropped in the miR-379 inhibitor + EIF4G2 shRNA group. The proliferation, migration, and invasion abilities of OS cells were reduced in the miR-379 mimics and EIF4G2 shRNA groups. The percentage of OS cells at the G0/G1 stage was increased, and the percentage at the S-stage was decreased in the miR-379 mimics and EIF4G2 shRNA groups. miR-379 may inhibit the proliferation, migration and invasion of OS cells through the down-regulation of EIF4G2.

MicroRNA‐379 inhibits laryngeal carcinoma cell proliferation and invasion through directly targeting TCF‐4

“…Deregulation of miRNAs has been demonstrated to contribute to cancer progression . In the current study, we paid attention to miR‐379, which has been observed in non‐small cell lung cancer, cervical cancer, breast cancer, and osteosarcoma . Nevertheless, no report on the biological roles of miR‐379 alterations in laryngeal carcinoma has been characterized.…”

“…26 In the current study, we paid attention to miR-379, which has been observed in non-small cell lung cancer, cervical cancer, breast cancer, and osteosarcoma. [16][17][18][19] Nevertheless, no report on the biological roles of miR-379 alterations in laryngeal carcinoma has been characterized. We focused on miR-379 as a candidate anti-oncogene in laryngeal carcinoma.…”

“…Increasing studies demonstrated that miRNA was aberrantly expressed in several tumors such as gastric cancer, bladder cancer, breast cancer, pancreatic cancer, and laryngeal squamous cell carcinoma . MiR‐379, located at chromosome 14q32.31, is differentially expressed in many kinds of cancers, suggesting that miR‐379 may exert key functions in cancer development. Nevertheless, little is reported about the biological functions of miR‐379 in laryngeal carcinoma.…”

“…15 MiR-379, located at chromosome 14q32. 31, is differentially expressed in many kinds of cancers, [16][17][18][19] suggesting that miR-379 may exert key functions in cancer development. Nevertheless, little is reported about the biological functions of miR-379 in laryngeal carcinoma.…”

CXCR4‐mediated osteosarcoma growth and pulmonary metastasis is suppressed by MicroRNA‐613

“…23,24 They have the potential to regulate various critical biological processes including proliferation, migration and apoptosis of different kind of cancer cells. 25,26 Previous studies have indicated that many miRNAs, such as miR-19a, 27 miR-610, 28 CXCR4 is a well-studied G-protein coupled chemokine receptor and commonly expressed on lots of cell types including osteosarcoma cells. 34 The binding of SDF-1 to CXCR4 results in divergent downstream signaling pathways that mediate cell proliferation, migration and apoptosis.…”