MicroRNA-378 contributes to osteoarthritis by regulating chondrocyte autophagy and bone marrow mesenchymal stem cell chondrogenesis

Feng, Lu; Yang, Zhengmeng; Li, Yucong; Pan, Qi; Zhang, Xiaoting; Wu, Xiaomin; Lo, Jessica; Wang, Haixing; Bai, Shanshan; Lu, Xuan; Wang, Ming; Lin, Sien; Pan, Xiaohua; Li, Gang

doi:10.1016/j.omtn.2022.03.016

Cited by 19 publications

(6 citation statements)

References 41 publications

(48 reference statements)

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“…Recent studies have shown that the level of autophagy in OA cartilage is reduced ( Feng et al, 2020 ), and that autophagy can protect chondrocytes from degradation ( Carames et al, 2012 ). BS induces several genes promoting autophagy (FOXO1, RUBCNL, PLEKHM1, and SQSTM1), autophagy markers like ATG2A, and genes improving lysosomal functions like LAMP2, all of which would be beneficial in OA context ( Kurakazu et al, 2019 ; Zhao et al, 2019 ; Ansari et al, 2021 ; Feng et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Curcuma longa and Boswellia serrata Extracts Modulate Different and Complementary Pathways on Human Chondrocytes In Vitro: Deciphering of a Transcriptomic Study

et al. 2022

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Objectives:Curcuma longa (CL) and Boswellia serrata (BS) extracts are used to relieve osteoarthritis symptoms. The aim of this in vitro study was to investigate their mechanisms of action at therapeutic plasmatic concentrations on primary human osteoarthritic (OA) chondrocytes.Methods: BS (10–50 μg/ml) and CL (0.4–2 μg/ml corresponding to 1–5 µM of curcumin) were evaluated separately or in combination on primary chondrocytes isolated from 17 OA patients and cultured in alginate beads. Ten patients were used for RNA-sequencing analysis. Proteomic confirmation was performed either by immunoassays in the culture supernatant or by flow cytometry for cell surface markers after 72 h of treatment.Results: Significant gene expression modifications were already observed after 6 h of treatment at the highest dose of CL (2 μg/ml) while BS was significantly effective only after 24 h of treatment irrespective of the concentration tested. The most over-expressed genes by CL were anti-oxidative, detoxifying, and cytoprotective genes involved in the Nrf2 pathway. Down-regulated genes were principally pro-inflammatory cytokines and chemokines. Inversely, BS anti-oxidant/detoxifying activities were related to the activation of Nrf1 and PPARα pathways. BS anti-inflammatory effects were associated with the increase in GDF15, decrease in cholesterol cell intake and fatty acid metabolism-involved genes, and down-regulation of Toll-like receptors (TLRs) activation. Similar to CL, BS down-regulated ADAMTS1, 5, and MMP3, 13 genes expression. The combination of both CL and BS was significantly more effective than CL or BS alone on many genes such as IL-6, CCL2, ADAMTS1, and 5.Conclusion: BS and CL have anti-oxidative, anti-inflammatory, and anti-catabolic activities, suggesting a protective effect of these extracts on cartilage. Even if they share some mechanism of action, the two extracts act mainly on distinct pathways, and with different time courses, justifying their association to treat osteoarthritis.

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Section: Discussionmentioning

confidence: 99%

Curcuma longa and Boswellia serrata Extracts Modulate Different and Complementary Pathways on Human Chondrocytes In Vitro: Deciphering of a Transcriptomic Study

et al. 2022

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“…Micromasses of rBMSCs and hBMSCs from 3 different donors constructed by 2 × 10 5 cells were obtained as previous established [ 83 ]. Blank hydrogels, MESS hydrogels and PE-MESS hydrogels, 30 μL each, were formed on the top of the transwell membrane.…”

Section: Experimental Section/methodsmentioning

confidence: 99%

O-alg-THAM/gel hydrogels functionalized with engineered microspheres based on mesenchymal stem cell secretion recruit endogenous stem cells for cartilage repair

Wang

et al. 2023

Bioactive Materials

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“…After 80% confluency, the cells were co-transfected with TOPFlash or NF-κB RE reporter vector, together with Renilla vector, respectively. The cells were then treated with chemicals from library as mentioned before at a concentration of 10 μM for 24 h. The dual-luciferase assay was then conducted in a PerkinElmer VictorTM X2 2030 multilateral reader (Waltham, MA, USA) according to the instructions as described previously [ 30 ]. The ratio of firefly luciferase of each treatment was normalized with Renilla activity.…”

Section: Methodsmentioning

confidence: 99%

Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis

Yang

Hou

et al. 2023

IJMS

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Osteoporosis, a common systematic bone homeostasis disorder related disease, still urgently needs innovative treatment methods. Several natural small molecules were found to be effective therapeutics in osteoporosis. In the present study, quercetin was screened out from a library of natural small molecular compounds by a dual luciferase reporter system. Quercetin was found to upregulate Wnt/β-catenin while inhibiting NF-κB signaling activities, and thereby rescuing osteoporosis-induced tumor necrosis factor alpha (TNFα) impaired BMSCs osteogenesis. Furthermore, a putative functional lncRNA, Malat1, was shown to be a key mediator in quercetin regulated signaling activities and TNFα-impaired BMSCs osteogenesis, as mentioned above. In an ovariectomy (OVX)-induced osteoporosis mouse model, quercetin administration could significantly rescue OVX-induced bone loss and structure deterioration. Serum levels of Malat1 were also obviously rescued in the OVX model after quercetin treatment. In conclusion, our study demonstrated that quercetin could rescue TNFα-impaired BMSCs osteogenesis in vitro and osteoporosis-induced bone loss in vivo, in a Malat1-dependent manner, suggesting that quercetin may serve as a therapeutic candidate for osteoporosis treatment.

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MicroRNA-378 contributes to osteoarthritis by regulating chondrocyte autophagy and bone marrow mesenchymal stem cell chondrogenesis

Cited by 19 publications

References 41 publications

Curcuma longa and Boswellia serrata Extracts Modulate Different and Complementary Pathways on Human Chondrocytes In Vitro: Deciphering of a Transcriptomic Study

Curcuma longa and Boswellia serrata Extracts Modulate Different and Complementary Pathways on Human Chondrocytes In Vitro: Deciphering of a Transcriptomic Study

O-alg-THAM/gel hydrogels functionalized with engineered microspheres based on mesenchymal stem cell secretion recruit endogenous stem cells for cartilage repair

Long Non-Coding RNA Malat1 Increases the Rescuing Effect of Quercetin on TNFα-Impaired Bone Marrow Stem Cell Osteogenesis and Ovariectomy-Induced Osteoporosis

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