microRNA‐377‐3p downregulates the oncosuppressor T‐cadherin in colorectal adenocarcinoma cells

Palo, Armando Di; Siniscalchi, Chiara; Polito, Rita; Nigro, Ersilia; Russo, Aniello; Daniele, Aurora; Potenza, Nicoletta

doi:10.1002/cbin.11605

Cited by 3 publications

(3 citation statements)

References 35 publications

(51 reference statements)

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“…After performing bioinformatics analysis, we identified miR‐377‐3p as potential miRNA that targets SYK. Previous studies have indicated that miR‐377‐3p exerts tumor inhibitory effects across various cancers 53–55 . Consistently, we observed significant downregulation of miR‐377‐3p expression in PTC.…”

Section: Discussionsupporting

confidence: 89%

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Spleen tyrosine kinase facilitates the progression of papillary thyroid cancer regulated by the hsa_circ_0006417/miR‐377‐3p axis

Tan,

Zheng,

Zhou

et al. 2023

Environmental Toxicology

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Papillary thyroid cancer (PTC) is a prevalent malignancy worldwide. Spleen tyrosine kinase (SYK) is a crucial enzyme that participates in various biological processes, including cancer progression. This study aims to uncover the biological function of SYK in PTC. SYK expression patterns in PTC were evaluated using quantitative real time polymerase chain reaction (qRT‐PCR), immunohistochemistry (IHC), and western blot. Cell function assays were performed to assess the effects of SYK on PTC. Bioinformatics analysis was conducted to identify intriguing microRNA (miRNA) and circular RNA (circRNA). Dual‐Luciferase Reporter or RNA immunoprecipitation assays were used to investigate the correlation among SYK, miR‐377‐3p, and hsa_circ_0006417. SYK was upregulated in PTC. Overexpression of SYK exhibited a positive correlation with tumor size, lymph node metastasis, and unfavorable disease‐free survival. Functional assays revealed that SYK exerted tumorigenic effect on PTC cells through mTOR/4E‐BP1 pathway. Mechanistically, hsa_circ_0006417 and miR‐377‐3p regulated SYK expression, offering modulating its tumor‐promoting effects. Collectively, SYK acts as an oncogene in PTC through mTOR/4E‐BP1 pathway, which is regulated by the hsa_circ_0006417/miR‐377‐3p axis, thereby providing a potential alternative for PTC treatment.

show abstract

Section: Discussionsupporting

confidence: 89%

“…Previous studies have indicated that miR-377-3p exerts tumor inhibitory effects across various cancers. [53][54][55] Consistently, we observed significant downregulation of miR-377-3p expression in PTC. Additionally, miR-377-3p was confirmed to direct bind to SYK mRNA.…”

Section: Discussionsupporting

confidence: 79%

Spleen tyrosine kinase facilitates the progression of papillary thyroid cancer regulated by the hsa_circ_0006417/miR‐377‐3p axis

Tan,

Zheng,

Zhou

et al. 2023

Environmental Toxicology

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“…Most miRNAs are inhibitory and can lead to the degradation of mRNAs, which decreases the expressions of target proteins. Many cytoplasmic miRNAs have been discovered that can regulate expression of N-cadherin, Vimentin, MMPs, E-cadherin, Claudin, and EpCAM, thereby influencing the direction of EMT through molecular regulation in targeted pathways ( 67 – 69 ). Go et al.…”

Section: Ncrnas Regulate the Emt Process In Crcmentioning

confidence: 99%

The Interaction Between Epigenetic Changes, EMT, and Exosomes in Predicting Metastasis of Colorectal Cancers (CRC)

2022

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Worldwide, colorectal cancer (CRC) ranks as the third most common malignancy, and the second most deadly with nearly one million attributable deaths in 2020. Metastatic disease is present in nearly 25% of newly diagnosed CRC, and despite advances in chemotherapy, less than 20% will remain alive at 5 years. Epigenetic change plays a key role in the epithelial-to-mesenchymal transition (EMT), which is a crucial phenotype for metastasis and mainly includes DNA methylation, non-coding RNAs (ncRNAs), and N6-methyladenosine (m6A) RNA, seemingly valuable biomarkers in CRCs. For ncRNAs, there exists a “molecular sponge effect” between long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs). The detection of exosomes is a novel method in CRC monitoring, especially for predicting metastasis. There is a close relationship between exosomes and EMT in CRCs. This review summarizes the close relationship between epigenetic changes and EMT in CRCs and emphasizes the crucial function of exosomes in regulating the EMT process.

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A Novel ceRNET Relying on the lncRNA JPX, miR-378a-3p, and Its mRNA Targets in Lung Cancer

Mosca,

Pezzullo,

De Leo

et al. 2024

Cancers

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Lung cancer is the leading cause of cancer-related death worldwide. Non-coding RNAs are emerging as critical players for the onset and progression of cancer. Analyses of three different datasets revealed that the lncRNA JPX was overexpressed in adenocarcinoma tissues in comparison to normal lungs, as expected for an oncogene. Intriguingly, the predicted binding miR-378a-3p showed a significant inverse correlation with JPX expression. The lncRNA/miRNA physical interaction was validated by reporter vectors. Then, the oncogenic activity of JPX, the tumor-suppressive role of miR-378a-3p, and the contribution of their functional interaction to cancer hallmarks were demonstrated using assays for cell proliferation, migration, invasion, and 3D-spheroid formation. Finally, molecular circuits were investigated by boosting the expression of both JPX and miR-378a-3p, singularly and in combination, demonstrating that JPX counteracted miR-378a-3p silencing activity toward its oncogenic targets GLUT1, NRP1, YY1, and Wnt5a. Overall, the data unveil a novel ceRNET (competing endogenous RNA network), wherein JPX acts as a ceRNA by binding to miR-378a-3p, thus reducing the miRNA silencing activity toward its downstream targets, and eliciting oncogenic pathways driving lung cancer. The knowledge of the network may pave the way to develop new diagnostic panels, and innovative RNA-targeted and RNA-based therapeutic strategies.

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microRNA‐377‐3p downregulates the oncosuppressor T‐cadherin in colorectal adenocarcinoma cells

Cited by 3 publications

References 35 publications

Spleen tyrosine kinase facilitates the progression of papillary thyroid cancer regulated by the hsa_circ_0006417/miR‐377‐3p axis

Spleen tyrosine kinase facilitates the progression of papillary thyroid cancer regulated by the hsa_circ_0006417/miR‐377‐3p axis

The Interaction Between Epigenetic Changes, EMT, and Exosomes in Predicting Metastasis of Colorectal Cancers (CRC)

A Novel ceRNET Relying on the lncRNA JPX, miR-378a-3p, and Its mRNA Targets in Lung Cancer

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