MicroRNA-374a protects against myocardial ischemia-reperfusion injury in mice by targeting the MAPK6 pathway

Huang, Zhong; Xu, Wei; Wu, Jinlei; Xiong, Lei; Chen, Xi-Ming

doi:10.1016/j.lfs.2019.116619

Cited by 71 publications

(43 citation statements)

References 31 publications

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“…Myocardial I/R injury often causes oxidative stress and inflammation, which in turn, leads to apoptosis and necrosis of cardiomyocytes. 60,61 Nesfatin-1 can prevent this injury by reducing the infarct size and post-ischemic contracture, and by inhibiting the release of lactate dehydrogenase 59 through its antioxidant properties. Myocardial infarction (MI) is one of the most common life-threatening diseases, which may lead to other diseases through oxidative stress and inflammation.…”

Section: Cardiac Diseasesmentioning

confidence: 99%

<p>Antioxidant, Anti-Inflammatory and Anti-Apoptotic Activities of Nesfatin-1: A Review</p>

Xu¹,

Chen²

2020

JIR

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Nesfatin-1, a newly identified energy-regulating peptide, is widely expressed in the central and peripheral tissues, and has a variety of physiological activities. A large number of recent studies have shown that nesfatin-1 exhibits antioxidant, antiinflammatory, and anti-apoptotic properties and is involved in the occurrence and progression of various diseases. This review summarizes current data focusing on the therapeutic effects of nesfatin-1 under different pathophysiological conditions and the mechanisms underlying its antioxidant, anti-inflammatory, and anti-apoptotic activities.

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Section: Cardiac Diseasesmentioning

confidence: 99%

<p>Antioxidant, Anti-Inflammatory and Anti-Apoptotic Activities of Nesfatin-1: A Review</p>

Xu¹,

Chen²

2020

JIR

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“…The blood ow to the heart is interrupted by ligating LAD, and then the blood supply is restored after a certain time. Zhaoqi H. et al used c57BL6/J mice to establish a MIRI model to study the protective effect of microRNA-374a targeting the MAPK6 pathway [18]. Xuehui Z. use ICR mice to establish a MIRI model to study inhibition of the JNK and p38 MAPK by Rosa rugosa avonoids to reduce myocardial ischemia-reperfusion injury [19].…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Shuxin Shengmai Dan on Myocardial Ischemia-Reperfusion Injury in Rats

Wang¹,

Hao²,

Piao³

et al. 2020

Preprint

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Background: The traditional Chinese medicine Shuxin Shengmai Dan (SXSMD) is clinically used to treat angina pectoris. The mechanism of action of SXSMD protection of the heart involves inhibition of inflammation and remains poorly understood. The role of SXSMD in rats with myocardial ischemia reperfusion (IR) and the mechanism of SXSMD action were studied in this research.Methods: The rats were treated with SXSMD (3.38, 6.76, and 13.52 g/kg/day, p.o.) or Danshen injecta (1.8 mL/kg/day, p.o.) for 15 days, then the coronary arteries were ligated. Cardiac function was evaluated by electrocardiography and hemodynamic measurements. Hematoxylin-eosin (H&E) staining was used to detect pathological changes in ischemic myocardial sections. Transmission electron microscopy (TEM) was used to assess the ultrastructure of cardiomyocytes. The changes in IL-6 and TNF-α in the rat serum were detected by ELISA. The changes in the expression levels of HMGB1, TLR4, MyD88, and NF-κB mRNAs and proteins related to the TLR4/NF-κB pathway in myocardial tissue were detected by qPCR and Western blot, respectively.Results: Rats with coronary artery ligation had abnormal cardiac function, inflammatory infiltration of myocardial cells, disordered myocardial fiber arrangement, accumulation of mitochondria, and disordered muscle fibers and sarcomeres according to electron microscopy. The levels of the expression of mRNAs and proteins in myocardial tissue of the SXSMD group were decreased compared with those in the MIRI group. The serum levels of IL-6 and TNF-α were decreased. SXSMD treatment can inhibit the inflammatory response and downregulate the TLR4/NF-κB pathway in cardiomyocytes.Conclusion: SXSMD protects the rats from myocardial ischemia-reperfusion injury in the MIRI model by downregulating the TLR4/NF-κB pathway to inhibit inflammation.

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“…miR-17-5p, miR-378b, miR-199a-5p, and miR-7 may have key roles to play in muscle aging [88], and upregulation of miR-17-5p has been demonstrated to contributes to hypoxia-induced proliferation in human pulmonary artery smooth muscle cells through modulation of p21 and PTEN [52]. Huang et al stated that miR-374a-5p was shown to negatively regulate MAPK6 expression, and miR-374a-5p may have protective effects against cardiac I/R injury in vivo, and H9C2 H/R injury in vitro [89]. Also, miR-129-5p blocks proliferation of vascular smooth muscle cells (VSMC) by decreasing the expression of Wnt5a [90] and cyclin-dependent kinase 6 (CDK6) cardiac myocytes [91].…”

Section: Discussionmentioning

confidence: 99%

CDR1as related miRNA-mRNA networks in differentiating goat skeletal muscle satellite cells

Kyei

Liu

et al. 2020

Preprint

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Background: Myogenesis is a complex process controlled by several coding and non-coding RNAs (ncRNAs) such as circular RNAs (circRNAs) that well-known function as endogenous microRNAs (miRNAs) sponges. Over the past few years, numerous circRNAs have been known and their roles in biological processes have begun to be understood. Cerebellar Degeneration-Related protein 1 antisense (CDR1as), the most spotlighted circRNA as miR-7 sponge that has been blooming circRNAs’ research for a decade, and can potentially sponge several miRNAs in disease and muscle physiology. Nevertheless, the linear-RNAs-differed character that the acute interventions for circRNAs do not affect miRNAs levels, and has retarded the transcriptome-wide discovery of miRNAs sponged by. Therefore, the purpose of this study was to provide the transcriptomic effect of CDR1as during muscle differentiation.Methods: siCDR1as and siDICER1 were transfected into goat skeletal muscle satellite cells (SMSCs). RNA-seq technology and bioinformatics tools were used to analyze genes that are deregulated by siCDR1as and siDICER1. quantitative PCR was used to verify the expression levels of the differentially expressed mRNAs and miRNAs. Results: Here, to systematically identify miRNAs targeting CDR1as, we employed the critical enzyme DICER1 that governs the biogenesis of miRNAs. The deficiency of either DICER1 or CDR1as inhibited myogenic differentiation of SMSCs, and knockdown of DICER1 decreased the expression of CDR1as. Moreover, we screened for the targeted messenger RNAs (mRNAs) and miRNAs in SMSCs transfected with siDICER1 or siCDR1as respectively and found out that some well-known muscle-related pathways such as phosphoinositide 3-kinase (PI3K)-AKT signaling pathway, Rap1 signaling pathway, and MAPK signaling pathway were enriched in all groups. Further, regarding the miRNAs identified in siDICER1 and siCDR1as together with the sequence complementary information, we identified 11 miRNAs including miR-1, miR-206, and miR-27a-5p which are more likely to be novel targets for CDR1as. Conclusion: In summary, our study provides a perspective on the potential functions and relationship between CDR1as and DICER1 during muscle development.

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MicroRNA-374a protects against myocardial ischemia-reperfusion injury in mice by targeting the MAPK6 pathway

Cited by 71 publications

References 31 publications

<p>Antioxidant, Anti-Inflammatory and Anti-Apoptotic Activities of Nesfatin-1: A Review</p>

<p>Antioxidant, Anti-Inflammatory and Anti-Apoptotic Activities of Nesfatin-1: A Review</p>

The Protective Effect of Shuxin Shengmai Dan on Myocardial Ischemia-Reperfusion Injury in Rats

CDR1as related miRNA-mRNA networks in differentiating goat skeletal muscle satellite cells

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