MicroRNA-373 Inhibits Cell Proliferation and Invasion via Targeting BRF2 in Human Non-small Cell Lung Cancer A549 Cell Line

Wang, Lei; Qu, Junfeng; Zhou, Li; Liao, Fei; Wang, Ju

doi:10.4143/crt.2017.302

Cited by 32 publications

(26 citation statements)

References 33 publications

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“…miR-21 promoted cell growth and invasion by repressing phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN in colorectal cancer (13), similar to the impact of miR-21 on gastric cancer (14). Recently, miR-373 was demonstrated to be an important gene in numerous human malignant tumors (15)(16)(17), which provoked widespread attention.…”

Section: Introductionmentioning

confidence: 77%

miR-373 suppresses gastric cancer metastasis by downregulating vimentin

Shi

Zhang

et al. 2017

Mol Med Report

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MicroRNA-373 (miR-373) has been reported to be an oncogene in a number of solid human tumors. However, the role of miR‑373 in gastric cancer has not been completely elucidated and the mechanisms remain unclear. In the present study, we compared miR‑373 expression between clinical gastric cancer tissues and paired non‑tumorous tissues by reverse transcription‑quantitative polymerase chain reaction. The impact of miR‑373 on proliferation, migration and invasion in gastric cancer cells was additionally investigated. Hsa‑miR‑373 mimics were applied to mimic the function of endogenous miR‑373. A colony formation assay and flow cytometry were performed to analyze the proliferation of gastric cancer cells. Wound healing and Transwell invasion assays were employed to detect the migratory and invasive abilities of gastric cancer cells. Western blotting was used to test the expression of epithelial‑mesenchymal transition‑associated proteins. The results demonstrated that the level of miR‑373 in gastric cancer was upregulated compared with paired non‑tumorous tissues. It was confirmed that miR‑373 inhibited the migration and invasion of the gastric cancer cell lines SGC‑7901 and HGC‑27 by downregulating vimentin expression. The results of the present study demonstrated an oncogenic role of miR‑373 in the metastasis of human gastric cancer, and may provide a novel therapeutic strategy for gastric cancer.

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Section: Introductionmentioning

confidence: 77%

miR-373 suppresses gastric cancer metastasis by downregulating vimentin

Shi

Zhang

et al. 2017

Mol Med Report

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“…As for prognosis, five studies (n=538) were prospective studies of the impact of miR-373 on the survival rate of cancer patients. 15,19,23,24,30 The data related to miR-372 was extracted from five studies (n=580). 22,25,[27][28][29] Moreover, one study (n=100) contained both miR-372 and miR-373 for prognostic value.…”

Section: Eligible Studiesmentioning

confidence: 99%

“…14 However, there are some reports holding different attitudes that down regulation of miR-373 in non-small-cell-lung-cancer (NSCLC) cells was observed to be associated with suppression of the cell EMT, proliferation, migration, and invasion. 15 Therefore, the role of miR-371-3 in development and progression of cancers remains obscure. In addition, the cluster was also reported as a diagnostic or prognostic biomarker, but the conclusions have remained elusive.…”

Section: Introductionmentioning

confidence: 99%

“…Such a varied result was arose from the different sample size, cutoff value, or cancer types. On the other hand, studies have evaluated the prognostic value of miR-372 and miR-373 for cancers, and reported they could serve as favorable survival indicators, 15,19,[22][23][24] as well as adverse survival indicators. [25][26][27][28][29][30] Therefore, the diagnostic and prognostic values of miR-371-3 cluster are unclear currently.…”

Section: Introductionmentioning

confidence: 99%

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<p>MicroRNA-371-3 cluster as biomarkers for the diagnosis and prognosis of cancers</p>

Pan¹,

He²,

Xu³

et al. 2019

CMAR

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To date, increasing evidences have demonstrated that the aberrant expression of miR-371-3 cluster has been verified in various cancers and could be potentially used as a biomarker for tumor diagnosis and prognosis. To explore the role of miR-371-3 cluster in tumor diagnosis and prognosis, we conducted this study based on the published data. Methods: We searched electronic databases (PubMed, EMBASE and Web of Science databases) (Jan 1, 2007 to Jun 1, 2018). The pooled sensitivity, specificity and area under the curve (AUC) of summary receiver operator characteristic (SROC) curve were used for diagnostic values, meanwhile the pooled hazard ration (HR) and 95% CI were used to explore the prognosis capacity of miR-372 and miR-373. In addition, the publication bias of the enrolled studies was tested and a sensitivity analysis of each study was performed to evaluate the stability of the pooled result. Results: A total of eleven eligible studies containing six eligible studies containing 870 participants for diagnosis and 1218 cancer cases for prognosis were selected for this study. For diagnosis, the pooled results revealed that the miR-371 (sensitivity: 0.85, specificity: 0.92, AUC: 0.92) and miR-373 (sensitivity: 0.81, specificity: 0.93, AUC: 0.93) could be used as diagnostic biomarkers. For prognosis, we observed that elevated miR-372 indicated poor prognosis (HR=2.31, 95% CI: 1.04-5.14), especially in the cutoff value subgroup of median (HR=2.62, 95% CI: 1.54-4.46). In addition, pooled results showed that expression of miR-373 was not related to prognosis because of the significant heterogeneity, and the high miR-373 expression presented favorable prognosis in Asians (HR=0.34, 95% CI: 0.23-0.50) after omitting the study of heterogeneity origin. Conclusion: The current studies demonstrated that miR-371 and miR-373 could be predictive tumor diagnostic biomarkers and the expression of miR-372 and miR-373 may indicate prognosis of cancer patients.

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“…Noncoding RNA have no ability to encode proteins, including small nuclear RNA (snRNA), long noncoding RNA (lncRNA), microRNA (miRNA), and so on, having many important regulatory functions in cells and participating in cell proliferation, differentiation, apoptosis, canceration, cancer progression, and other cellular processes . Now compelling evidence finds that the dysregulation of CSCs in various cancers have something to do with abnormal expression of miRNAs.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of lung cancer: A two‐miRNA prognostic signature based on cancer stem‐like cells related genes

Yan-ping

Yang

Shen

et al. 2019

J of Cellular Biochemistry

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Lung cancer is one of the deadliest cancers worldwide. To increase the survival rate of lung cancer, it is necessary to explore specific prognosis markers. More and more evidence finds that noncoding RNA is closely associated with the survival of lung cancer, and cancer stem cells (CSCs) also play a significant role in the progress of lung cancer. The objective of this study is to find CSLCs genes that affect the prognosis of lung cancer. The differential expression of long noncoding RNAs (lncRNAs), microRNAs (miRNAs), messenger RNAs (mRNAs) in the Cancer Genome Atlas (TCGA) database and differential expression data from microarray of CD326+ and CD326− A549 cell are intersected to identify stable and consistent expression genes (2 lncRNAs, 15 miRNAs, and 134 mRNAs). The intersection of lncRNAs and miRNAs is analyzed by univariate and multivariate Cox regression to obtained prognostic genes. Two miRNAs (miR‐30b‐5p and miR‐29c‐3p) are significantly correlated with the overall survival rate. Then using these two miRNAs to construct a risk score model as a prognosis signature of lung cancer. Subsequently, we analyzed the association between two miRNAs and clinical information of lung cancer patients, of which T stage, Neoplasm cancer and risk score (P < .05) can be used as independent prognostic indicators of lung cancer. Finally, target genes of 2 miRNAs and 134 mRNAs were annotated with Gene Ontology and analyzed with Kyoto Encyclopedia of Genes and Genomes pathway, and verified with the GEO database. In summary, this study illustrates the role of miRNAs in the promotion of lung cancer by CSCs, which is important to find molecular biomarkers of lung cancer.

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MicroRNA-373 Inhibits Cell Proliferation and Invasion via Targeting BRF2 in Human Non-small Cell Lung Cancer A549 Cell Line

Cited by 32 publications

References 33 publications

miR-373 suppresses gastric cancer metastasis by downregulating vimentin

miR-373 suppresses gastric cancer metastasis by downregulating vimentin

<p>MicroRNA-371-3 cluster as biomarkers for the diagnosis and prognosis of cancers</p>

Molecular characterization of lung cancer: A two‐miRNA prognostic signature based on cancer stem‐like cells related genes

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