MicroRNA-370 suppresses proliferation and promotes endometrioid ovarian cancer chemosensitivity to cDDP by negatively regulating ENG

Chen, Xiaoping; Chen, Youguo; Lan, Jianyun; Shen, Zhi‐Liang

doi:10.1016/j.canlet.2014.07.026

Cited by 65 publications

(59 citation statements)

References 23 publications

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“…MiR-370 exerts different function in various tumors. In most cases, miR-370 is lowly expressed and exerts tumor-suppressive role in tumor cells (Lo et al, 2012; Chen et al, 2014). A latest study shows that miR-370 is down-regulated in human glioma tissues cells (Peng et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Knockdown of Long Non-Coding RNA KCNQ1OT1 Restrained Glioma Cells’ Malignancy by Activating miR-370/CCNE2 Axis

Gong

Zheng

Liu

et al. 2017

Front. Cell. Neurosci.

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Accumulating evidence has highlighted the potential role of long non-coding RNAs (lncRNAs) as biomarkers and therapeutic targets in solid tumors. Here, we elucidated the function and possible molecular mechanisms of lncRNA KCNQ1OT1 in human glioma U87 and U251 cells. Quantitative Real-Time polymerase chain reaction (qRT-PCR) demonstrated that KCNQ1OT1 expression was up-regulated in glioma tissues and cells. Knockdown of KCNQ1OT1 exerted tumor-suppressive function in glioma cells. Moreover, a binding region was confirmed between KCNQ1OT1 and miR-370 by dual-luciferase assays. qRT-PCR showed that miR-370 was down-regulated in human glioma tissue and cells. In addition, restoration of miR-370 exerted tumor-suppressive function via inhibiting cell proliferation, migration and invasion, while promoting the apoptosis of human glioma cells. Knockdown of KCNQ1OT1 decreased the expression level of Cyclin E2 (CCNE2) by binding to miR-370. Further, miR-370 bound to CCNE2 3′UTR region and decreased the expression of CCNE2. These results provided a comprehensive analysis of KCNQ1OT1-miR-370-CCNE2 axis in human glioma cells and might provide a novel strategy for glioma treatment.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: Knockdown of Long Non-Coding RNA KCNQ1OT1 Restrained Glioma Cells’ Malignancy by Activating miR-370/CCNE2 Axis

Gong

Zheng

Liu

et al. 2017

Front. Cell. Neurosci.

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“…Overexpression of miR-370 in vitro showed decreased proliferation and increased sensitivity to platinum. Similar results were revealed in xenograft studies with miR-370 (54). Thus, microRNA molecules may play significant roles in the underlying biology of endometrioid EOC.…”

Section: Microrna Molecules As Clinical Biomarkers For Eocsupporting

confidence: 85%

“…Multiple studies have highlighted the function of microRNA molecules in terms of endometrioid histotypes (52–54). Expression of miR-191 was increased in ovarian endometriosis and endometrioid EOC.…”

Section: Microrna Molecules As Clinical Biomarkers For Eocmentioning

confidence: 99%

The role of MicroRNA molecules and MicroRNA-regulating machinery in the pathogenesis and progression of epithelial ovarian cancer

Wang

Ivan

Hawkins

2017

Gynecologic Oncology

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MicroRNA molecules are small, single-stranded RNA molecules that function to regulate networks of genes. They play important roles in normal female reproductive tract biology, as well as in the pathogenesis and progression of epithelial ovarian cancer. DROSHA, DICER, and Argonaute proteins are components of the microRNA-regulatory machinery and mediate microRNA production and function. This review discusses aberrant expression of microRNA molecules and microRNA-regulating machinery associated with clinical features of epithelial ovarian cancer. Understanding the regulation of microRNA molecule production and function may facilitate the development of novel diagnostic and therapeutic strategies to improve the prognosis of women with epithelial ovarian cancer. Additionally, understanding microRNA molecules and microRNA-regulatory machinery associations with clinical features may influence prevention and early detection efforts.

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“…On the other hand, we have identified a downregulation of miR-199-5p and miR-370 in colon cancer cells, as it has been showed in other cancer types such as renal cancer, ovarian cancer, hepatocellular carcinoma, bladder cancer, and larynx cancer37383940. In addition, in endometrial ovarian cancer cells, miR-370 suppressed cell viability and colony formation and increased chemosensitivity41, being also downregulated in chemoresistant breast cancer cells42. The analysis of selected miRs in cancer stem-like subpopulations using the hsa-miR-24c-3p normalizer was previously published in Morata-Tarifa C et al .…”

Section: Discussionmentioning

confidence: 55%

Validation of suitable normalizers for miR expression patterns analysis covering tumour heterogeneity

Morata-Tarifa

Picón-Ruiz

Griñán‐Lisón

et al. 2017

Sci Rep

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Oncogenic microRNAs (miRs) have emerged as diagnostic biomarkers and novel molecular targets for anti-cancer drug therapies. Real-time quantitative PCR (qPCR) is one of the most powerful techniques for analyzing miRs; however, the use of unsuitable normalizers might bias the results. Tumour heterogeneity makes even more difficult the selection of an adequate endogenous normalizer control. Here, we have evaluated five potential referenced small RNAs (U6, rRNA5s, SNORD44, SNORD24 and hsa-miR-24c-3p) using RedFinder algorisms to perform a stability expression analysis in i) normal colon cells, ii) colon and breast cancer cell lines and iii) cancer stem-like cell subpopulations. We identified SNORD44 as a suitable housekeeping gene for qPCR analysis comparing normal and cancer cells. However, this small nucleolar RNA was not a useful normalizer for cancer stem-like cell subpopulations versus subpopulations without stemness properties. In addition, we show for the first time that hsa-miR-24c-3p is the most stable normalizer for comparing these two subpopulations. Also, we have identified by bioinformatic and qPCR analysis, different miR expression patterns in colon cancer versus non tumour cells using the previously selected suitable normalizers. Our results emphasize the importance of select suitable normalizers to ensure the robustness and reliability of qPCR data for analyzing miR expression.

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MicroRNA-370 suppresses proliferation and promotes endometrioid ovarian cancer chemosensitivity to cDDP by negatively regulating ENG

Cited by 65 publications

References 23 publications

RETRACTED: Knockdown of Long Non-Coding RNA KCNQ1OT1 Restrained Glioma Cells’ Malignancy by Activating miR-370/CCNE2 Axis

RETRACTED: Knockdown of Long Non-Coding RNA KCNQ1OT1 Restrained Glioma Cells’ Malignancy by Activating miR-370/CCNE2 Axis

The role of MicroRNA molecules and MicroRNA-regulating machinery in the pathogenesis and progression of epithelial ovarian cancer

Validation of suitable normalizers for miR expression patterns analysis covering tumour heterogeneity

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