MicroRNA-34a inhibits tumor invasion and metastasis in osteosarcoma partly by effecting C-IAP2 and Bcl-2

Wen, Jie; Zhao, Yan-Kun; Liu, Yan; Zhao, Jinfeng

doi:10.1177/1010428317705761

Cited by 21 publications

(20 citation statements)

References 34 publications

(42 reference statements)

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“…In addition, miR-34a was regarded as a novel prognostic biomarker and correlated with tumor size and stage in OS ( 45 ). The present study reported that miR-34a was downregulated in OS tissues and cells, in accordance with previous studies ( 24 , 25 ). Additionally, MALAT1 was negatively correlated with miR-34a expression in OS cells.…”

Section: Discussionsupporting

confidence: 94%

“…Recently, increasing evidence has demonstrated that lncRNAs could function as competing endogenous RNAs (ceRNAs) to suppress the expression and activities of miRNAs, thus regulating the expression of target mRNA ( 21 - 23 ). As a well-studied miRNA, miR-34a, a member of the miR-34 family, has been reported as downregulated in OS cells and functioned as a tumor suppressor in the pathogenesis of OS ( 24 , 25 ). Considering the importance of MALAT1 and miR-34a in the pathogenesis of OS, the present study investigated whether MALAT1 functions as a molecular sponge of miR-34a in OS.…”

Section: Introductionmentioning

confidence: 99%

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Knockdown of MALAT1 inhibits osteosarcoma progression via regulating the miR‑34a/cyclin D1 axis

Duan

Zhang

et al. 2018

Int J Oncol

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Long non-coding (lnc)RNAs have been demonstrated to be involved in the development of various types of cancers, such as osteosarcoma (OS). Long non-coding (lnc) RNA metastasis associated lung adenocarcinoma transcript 1 (MALAT1) expression was reported to be highly expressed in OS and promoted the development of this disease; however, the underlying molecular mechanism by which MALAT1 promotes the progression of OS requires further investigation. In the present study, the expression of MALAT1 and miR-34a was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The abundance of cyclin D1 (CCND1) was detected by RT-qPCR and western blotting. Cell viability, migration and invasion were examined by MTT and Transwell assays. The interaction between miR-34a and MALAT1 or CCND1 was probed by a dual luciferase reporter assay and RNA immunoprecipitation. Xenograft tumor assay was performed to verify the roles of MALAT1 and miR-34a in tumor growth in vivo. The results demonstrated that MALAT1 and CCND1 mRNA expression levels were upregulated and miR-34a was downregulated in OS tissues and cells. Additionally, MALAT1 expression was correlated with tumor size, clinical stage and distant metastasis in patients with OS. In addition, MALAT1 promoted OS cell viability, invasion and migration, while MALAT1 silencing exhibited opposing effects. Moreover, MALAT1 functioned as a ceRNA to suppress miR-34a expression and in turn upregulate CCND1 in OS cells. Rescue experiments further demonstrated that MALAT1 knockdown partially reversed anti-miR-34a-mediated promotion on OS cell viability, migration and invasion; overexpression of CCND1 partially reversed the effects of MALAT1 silencing on OS progression. Furthermore, in vivo experiments also revealed that MALAT1 promoted OS tumor growth via miR-34a inhibition and upregulating the expression of CCND1. In conclusion, the present study suggested that MALAT1 exerted its oncogenic function in OS by regulating the miR-34a/CCND1 axis in OS, which may provide novel insight into the diagnosis and therapy for OS.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Knockdown of MALAT1 inhibits osteosarcoma progression via regulating the miR‑34a/cyclin D1 axis

Duan

Zhang

et al. 2018

Int J Oncol

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“…BCL-2 acted as an apoptosis factor and was proven to take part in regulating some cancers targeted by miR-34a. It was reported by Wen et al that miR-34a inhibited osteosarcoma cell invasion and migration via targeting BCL-2 and C-IAP2 ( 32 ), which is similar to the study that miR-34a suppressed the viability and migration of breast cancer by inhibiting BCL-2 ( 33 ). Wang et al showed that miR-34a mimic curbed cell viability and facilitated the apoptosis of cervical cancer cell through targeting BCL-2 ( 15 ).…”

Section: Discussionsupporting

confidence: 85%

miR-34a targets BCL-2 to suppress the migration and invasion of sinonasal squamous cell carcinoma

Zhao

Xian-zhi

2018

Oncol Lett

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Sinonasal squamous cell carcinomas (SN-SCC) are rare tumors with low survival rate. It was reported that miR-34a expression is low in many cancers and acted as a tumor suppressor. But the biological function of miR-34a in SN-SCC has hardly been reported. Therefore, we explored the role and underlying mechanism of miR-34a in the migration and invasion of SN-SCC. Western blot analysis and RT-PCR were carried out to examine B-cell lymphoma-2 (BCL-2) and miR-34a expression in SN-SCC. Transwell assay was performed to test the SN-SCC migratory and invasive ability. Luciferase reporter assay was carried out to verify the target of miR-34a. Results demonstrated that miR-34a expression was lower in SN-SCC tissues and cells than normal SN-SCC. Re-expression of miR-34a inhibited cell migration and invasion, while had the opposite effect on inhibition of miR-34a. We also found that BCL-2 expression was higher in SN-SCC and silencing BCL-2 curbed the development of SN-SCC. BCL-2 was found to be a target of miR-34a and negatively correlated with miR-34a expression. Furthermore, BCL-2 attenuated the miR-34a inhibitory effect on SN-SCC cell migration and invasion. In short, these data demonstrated that miR-34a inhibited SN-SCC cell migration and invasion through targeting BCL-2.

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“…miRNA regulates the important pathophysiological processes in the development, progression, metastasis and chemotherapy of osteosarcoma, providing new ideas for targeted therapy of osteosarcoma [12]. miR-34a-5p [13] and miR-20a-5p [14] can increase the chemosensitivity of osteosarcoma cells; miR-34a inhibits tumor invasion and metastasis of osteosarcoma by affecting C-IAP2 and Bcl-2 [15]. In addition, miR-34a can also reduce the self-renewal ability, tumorigenic activity and invasive ability of cancer stem cells [16].…”

Section: Discussionmentioning

confidence: 99%

The study of mechanism of miR-34c-5p targeting FLOT2 to regulate proliferation, migration and invasion of osteosarcoma cells

Wang

Tang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Objective: Osteosarcoma is one of the most common malignancies in children and adolescents. Studies have shown that miR-34c-5p is involved in the progression of various cancers. To explore the effects of miR-34c-5p on the proliferation, migration and invasion of osteosarcoma cells and its potential mechanism. Methods: qRT-PCR was used to detect the expression levels of miR-34c-5p and FLOT2 mRNA in osteosarcoma tissues and cells. Western Blot was used to detect protein expression. MTT assay used to detect cell viability. Transwell was used to detect cell migration and invasion in each group. Dual luciferase reporter gene assay was used to detect luciferase activity. Results: The expression of miR-34c-5pwas significantly decreased in osteosarcoma tissues and cells and the expression level of FLOT2 mRNA was significantly increased. Overexpression of miR-34c-5p and inhibition of FLOT2 inhibited the proliferation, migration and invasion of osteosarcoma cells and inhibited the expression of Cyclin D1, MMP-2 and MMP-9 proteins and promoted the expression of p21 protein. miR-34c-5p targeted to regulate the expression of FLOT2. Overexpression of FLOT2 reversed the inhibitory effect of miR-34c-5p overexpression on proliferation, migration and invasion of osteosarcoma cell lines. Conclusion: miR-34c-5p can inhibit the proliferation, migration and invasion of osteosarcoma cells. The mechanism may be related to targeting FLOT2, which will provide a new target for the prevention and treatment of osteosarcoma.

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MicroRNA-34a inhibits tumor invasion and metastasis in osteosarcoma partly by effecting C-IAP2 and Bcl-2

Cited by 21 publications

References 34 publications

Knockdown of MALAT1 inhibits osteosarcoma progression via regulating the miR‑34a/cyclin D1 axis

Knockdown of MALAT1 inhibits osteosarcoma progression via regulating the miR‑34a/cyclin D1 axis

miR-34a targets BCL-2 to suppress the migration and invasion of sinonasal squamous cell carcinoma

The study of mechanism of miR-34c-5p targeting FLOT2 to regulate proliferation, migration and invasion of osteosarcoma cells

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