MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma

Abstract

A body of evidence has indicated that microRNAs (miRNAs) may have significant roles in cancer. Aberrant expression of miRNAs has frequently been observed in various human malignancies, including osteosarcoma (OS). However, the roles of miRNAs in OS remain poorly understood. In the present study, high-throughput deep sequencing was performed to screen for deregulated miRNAs in OS. Screening identified 310 miRNAs which were significantly overexpressed and 41 miRNAs which were significantly downregulated (>2-fold) in OS samples, compared with adjacent non-tumor bone tissues. Among these miRNAs, miR-33a-5p was notably downregulated. TaqMan reverse transcription-polymerase chain reaction analysis further verified that miR-33a-5p expression was significantly reduced in a large cohort of human OS samples. Enhancing miR-33a-5p expression via transfection with miR-33a-5p precursor significantly inhibited OS cell growth, suggesting potential antitumor properties of miR-33a-5p. The results of the present study provide novel insights into the miRNAs involved in OS, and suggest that miR-33a-5p may function as a tumor suppressor in OS. Therefore, miR-33a-5p may be able to serve as a diagnostic and therapeutic target for OS treatment.

miRNA profiling identifies deregulated miRNAs associated with osteosarcoma development and time to metastasis in two large cohorts

“…Hence, the focus of this study was the analysis of miRNA expression changes in OS. Profiling of miRNA expression in OS has been addressed in previous studies (Duan et al ., ; Jones et al ., ; Lulla et al ., ; Maire et al ., ; Namlos et al ., ; Thayanithy et al ., ; Won et al ., ; J. Zhang et al ., ). However, the limited number of samples of 2–18 OS samples in the discovery cohorts and 2–12 samples in the respective validation cohorts makes it challenging to draw reliable conclusions from the published data.…”

“…Of the 29 deregulated miRNAs identified in this study, expression changes in nine miRNAs have not previously been associated with OS in miRNA profiling studies, while 20 miRNAs previously associated with OS development and progression were uncovered (Duan et al ., ; Jones et al ., ; Lulla et al ., ; Maire et al ., ; Namlos et al ., ; Thayanithy et al ., ; Won et al ., ; Zhang et al ., ). However, as OS is characterized by many different alterations, confirmation of previous findings is important for this particular disease.…”

“…Of these, miR‐100‐5p, miR‐127‐3p, and miR‐335‐5p have previously been identified as downregulated in OS studies analyzing 2–18 samples (Jones et al ., ; Maire et al ., ; Thayanithy et al ., ; Zhang et al ., ). However, miR‐127‐3p and miR‐370‐3p have also been detected upregulated in two OS samples in one study (Zhang et al ., ). miR‐125b‐5p and miR‐411‐5p have not previously been identified having an altered expression in any OS miRNA profiling studies.…”

WITHDRAWN: Lack of miR-216b-5p, miR-33a-5p or miR-122-5p in MSCs exosomes promotes breast cancer glycolysis by targeting HK2, LDHA and PKM2

“…Our study for the first time verified that miR-216b-5p functioned as a tumor suppressing miRNA in breast cancer. MiR-33a-5p suppresses osteoscarcoma and hepatocellular carcinoma cell growth [21][22]. MiR-122-5p is down-regulated in breast cancer cell [23].…”

MicroRNA-33a-3p suppresses cell migration and invasion by directly targeting PBX3 in human hepatocellular carcinoma

“…With the in-depth research, vertebrate miRNA * was demonstrated to play an important regulatory role in biological processes. In a previous study, miR-33a was shown to functions as a tumor suppressor in multiple cancers, such as melanoma, breast cancer, and osteosarcoma [19–21]. Similar to miR-33a, here, our results demonstrate that the expression levels of miR-33a-3p were not only negatively associated with the metastatic phenotype in HCC cells and tissues, but also that miR-33a-3p plays a suppressor role in cell migration and metastasis of HCC.…”