MicroRNA‐320a inhibits invasion and metastasis in osteosarcoma by targeting cytoplasmic polyadenylation element‐binding protein 1

Wang, Yanlong; Yang, Jiyu; Chen, Pangtao; Song, Yu; An, Weizheng; Zhang, Haoran; Butegeleqi, Butegeleqi; Yan, Jinglong

doi:10.1002/cam4.2919

Cited by 20 publications

(20 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR-320a, which is an extensively studied miRNA, has been reported to serve a critical role in diabetic retinopathy (31) and atherosclerosis (32). Moreover, miR-320a has been investigated in several types of cancer, such as lung cancer (33), papillary thyroid cancer (34), osteosarcoma (35) and hepatocellular carcinoma (36). Moreover, miR-320a has been reported to be upregulated in retinoblastoma tissues (37); however its role and mechanism in retinoblastoma remain to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of microRNA‑320a inhibits proliferation and induces apoptosis of retinoblastoma cells via targeting TUSC3

Kong¹,

Sun²,

Chen³

et al. 2020

Exp Ther Med

View full text Add to dashboard Cite

MicroRNA (miR)-320a is specific to vertebrates and has been indicated to serve a role in a number of cancer types, such as gastric, colorectal, pancreatic and ovarian cancer. miR-320a has been reported to be expressed at high levels in retinoblastoma tissues; however its role and mechanism of function in retinoblastoma remain to be elucidated. The aim of the present study was to investigate the role of miR-320a in retinoblastoma cells and the underlying mechanisms. The expression of miR-320a in retinoblastoma cell lines Y79 and WERI-Rb-1, and normal human retinal pigment epithelial cell line ARPE-19 was examined via reverse transcription-quantitative PCR (RT-qPCR). TargetScan bioinformatics analysis and dual-luciferase reporter assay were used to predict and reveal the target gene of miR-320a. Target gene expression was detected via RT-qPCR in retinoblastoma cell lines and ARPE-19 cells. Subsequently, gain-or loss-of-function experiments for miR-320a and tumor suppressor candidate 3 (TUSC3) were performed to study the role of miR-320a/TUSC3 in retinoblastoma cells. Cell viability and apoptosis were assessed via MTT and flow cytometry analysis, respectively. Compared with ARPE-19 cells, miR-320a was prominently expressed in retinoblastoma cell lines. TUSC3 was predicted to be a target gene of miR-320a. Compared with ARPE-19 cells, the expression of TUSC3 in retinoblastoma cell lines was reduced. The results of MTT and flow cytometry analysis revealed that overexpression of TUSC3 reduced the viability of retinoblastoma cells and induced apoptosis. Additional analysis indicated that miR-320a inhibitor enhanced the expression of the target gene TUSC3, thereby inhibiting retinoblastoma cell viability and inducing apoptosis. The effects of miR-320a inhibitor on retinoblastoma cells were inhibited by TUSC3-short hairpin RNA. miR-320a regulated the viability and apoptosis of retinoblastoma cells via targeting TUSC3. Therefore, the present study provided a reference for investigating a potential target for the clinical treatment of retinoblastoma.

show abstract

Section: Introductionmentioning

confidence: 99%

Downregulation of microRNA‑320a inhibits proliferation and induces apoptosis of retinoblastoma cells via targeting TUSC3

Kong¹,

Sun²,

Chen³

et al. 2020

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…Numerous studies have shown that miRNA regulatory networks played an essential effect on the progression and chemoresistance of cancer [ 32 , 33 ]. Liu et al showed that miR-497-5p enhanced apoptosis in OC cells [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Circ_0005198 enhances temozolomide resistance of glioma cells through miR-198/TRIM14 axis

Deng

Zhu

Xiao

et al. 2020

Aging

View full text Add to dashboard Cite

Circular RNAs (circRNAs) are associated with chemoresistance in many cancers. However, the function of circ_0005198 in the temozolomide (TMZ) resistance of glioma has not been well elucidated. Here, we demonstrated that circ_0005198 was considerably up-regulated in glioma tissues, serum samples and TMZ-resistant glioma cells. Silencing of circ_0005198 restrained TMZ resistance, restricted the proliferation and facilitated the apoptosis of TMZ-resistant glioma cells. MiR-198 could be sponged by circ_0005198, and we demonstrated that the effect of circ_0005198 on the progression of TMZ-resistant glioma cells was attributed to the inhibition of miR-198 activity. Moreover, TRIM14 was a target of miR-198 and silencing of TRIM14 hindered TMZ resistance and suppressed the progression of TMZ-resistant glioma cells, while TRIM14 over-expression rescued the inhibiting effect of miR-198 over-expression. We conclude that circ_0005198-miR-198-TRIM14 regulatory pathway is critical to TMZ resistance of glioma.

show abstract

“…miR-320a was described as a tumor suppressor for osteosarcoma [9,11] and involved in osteoporotic bone [13], but its effect has never been compared between both cellular contexts. Hence, we overexpressed or inhibited miR-320a in two osteosarcoma cell lines, and the miRNA effects on these two cells lines were compared to that on primary hOBs.…”

Section: Discussionmentioning

confidence: 99%

“…This miRNA was also detected in serums as a circulating microRNA, and it was proposed as a potential biomarker for osteonecrosis of femoral head [5]. Moreover, this miRNA is mainly involved in cancer malignancies including osteosarcoma, in which it acts as a tumor suppressor [6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Effect of the Tumor Suppressor miR-320a on Viability and Functionality of Human Osteosarcoma Cell Lines Compared to Primary Osteoblasts

et al. 2020

View full text Add to dashboard Cite

The miR-320a regulates a number of genes involved in various physiological processes. In particular, it has been reported as a tumor suppressor in several types of human cancers and involved in osteoporotic fracture and osteoblast function. Hence, the role of miR-320a has been evaluated in tumor cells and in primary cells in a separated context, but its effect has never been explored in a comparative manner. The present study aims to evaluate the cellular effects of miR-320a on human osteosarcoma cell lines (MG-63 and U2OS) compared to that on primary human osteoblasts (hOBs). miR-320a was either overexpressed or inhibited in all cell lines, and cell proliferation and viability were analyzed. Additionally, the effects of miR-320a on matrix mineralization, alkaline phosphatase activity, and oxidative stress were also evaluated in order to assess osteoblast functionality. In osteosarcoma cells, miR-320a overexpression reduced cell viability and proliferation, while in hOB cell viability was not affected and proliferation even was increased. The overexpression of miR-320a in both osteosarcoma cells and hOBs reduced the mineralization capacity. Finally, an increased oxidative stress was detected in all cells after miR-320a overexpression mainly in osteosarcoma. In conclusion, the overexpression of miR-320a increased stress oxidation levels, which could be involved in the reduced osteoblast performance, even though the cell viability was only affected in osteosarcoma cells.

show abstract

MicroRNA‐320a inhibits invasion and metastasis in osteosarcoma by targeting cytoplasmic polyadenylation element‐binding protein 1

Cited by 20 publications

References 56 publications

Downregulation of microRNA‑320a inhibits proliferation and induces apoptosis of retinoblastoma cells via targeting TUSC3

Downregulation of microRNA‑320a inhibits proliferation and induces apoptosis of retinoblastoma cells via targeting TUSC3

Circ_0005198 enhances temozolomide resistance of glioma cells through miR-198/TRIM14 axis

Effect of the Tumor Suppressor miR-320a on Viability and Functionality of Human Osteosarcoma Cell Lines Compared to Primary Osteoblasts

Contact Info

Product

Resources

About