MicroRNA-320 family is downregulated in colorectal adenoma and affects tumor proliferation by targeting CDK6

Tadano, Toshihiro; Kakuta, Yoichi; Hamada, Shin; Shimodaira, Yosuke; Kuroha, Masatake; Kawakami, Yoko; Kimura, Tsutomu; Shiga, Hisashi; Endo, Katsuya; Masamune, Atsushi; Takahashi, Seiichi; Kinouchi, Yoshitaka; Shimosegawa, Tooru

doi:10.4251/wjgo.v8.i7.532

Cited by 60 publications

(55 citation statements)

References 31 publications

(33 reference statements)

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“…In this study, we investigated the function of miR-320b in glioma, detecting a greatly decreased expression pattern of miR-320b in glioma tissues compared with normal brain tissues as well as in both U87 and U251 glioma cell lines in contrast with NC, which was similar to the results by Tadano et al and Li et al in colorectal adenoma (31) and nasopharyngeal carcinoma patients (14). to further identify the clinical significance of miR-320b, we explored the association between miR-320b and clinicopathological features of glioma patients, finding that its expression was tightly related to tumor clinical stage, with low expression of miR-320b more frequently exhibited in high degree gliomas.…”

Section: No Of Patients --------------------------------------------supporting

confidence: 80%

“…Li et al reported that decrease of miRNA-320b enhanced NpC cell proliferation by targeting tRIAp1 (14). In addition, Wang et al and Tadano et al discovered that miR-320b could markedly promote cell proliferation ability of human colorectal cancer cells by targeting c-Myc and CDK6 both in vitro and in vivo (15,31). Similar results were presented in our study, since MTS and colony formation assays revealed that miRNA-320b mimics significantly suppressed cell proliferation both in U87 and U251 cell lines compared with NC group.…”

Section: No Of Patients --------------------------------------------mentioning

confidence: 99%

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Low expression of microRNA-320b correlates with tumorigenesis and unfavorable prognosis in glioma

Liu

et al. 2017

Oncology Reports

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Accumulating evidence demonstrates that dysregulated microRNAs (miRNAs) play a critical role in tumorigenesis and progression of various cancers. miR-320b, a member of miR‑320 family, was revealed downregulated in numerous human cancers, including nasopharyngeal carcinoma and colorectal cancer. However, the function of miR‑320b in human glioma remained poorly defined. In this study, we report that miR‑320b was lowly expressed in glioma tissues and cell lines in contrast with controls, being closely correlated with histological malignancy of glioma. Furthermore, patients with low expression of miR‑320b were associated with poor prognostic outcomes. In vitro functional assays indicated that overexpression of miR‑320b could markedly enhance cell apoptosis rate and suppress cell proliferation, migration and invasion. miR-320b mimic impaired cell cycle and metastasis through inhibiting the expression of G1/S transition key regulator Cyclin D1 as well as decreasing the expression level of MMP2 and MMP9. Additionally, upregulation of miR‑320b could markedly promote apoptosis by increasing the level of Bax and reducing Bcl-2 expression in glioma. Taken together, our data suggested that miR‑320b might serve as a novel prognostic marker and potential therapeutic target for glioma.

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Section: No Of Patients --------------------------------------------supporting

confidence: 80%

Section: No Of Patients --------------------------------------------mentioning

confidence: 99%

Low expression of microRNA-320b correlates with tumorigenesis and unfavorable prognosis in glioma

Liu

et al. 2017

Oncology Reports

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“…From microarray assay, Tadano et al. found that miR‐320 family including miR‐320d were downregulated in colorectal adenoma as well as submucosal invasive carcinoma tissues, and could inhibit cell proliferation . However, a next‐generation sequencing analysis research found that miR‐320d was with higher expression in primary colorectal cancer (pCRC) tissue compared to normal tissue, and was expressed significantly higher in metastatic lesion compared with those in pCRC tissue .…”

Section: Discussionmentioning

confidence: 99%

Downregulation of MicroRNA‐320d predicts poor overall survival and promotes the growth and invasive abilities in glioma

Qin

Yang

et al. 2016

Chem Biol Drug Des

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Previous studies have demonstrated that miRNAs play an important role in tumor development and progression. The role of miR-320d has been studied in several cancers except for glioma. The aim of the study was to investigate the expression levels, biological function, and mechanism of miR-320d in glioma. The expression levels of miR-320d were detected in glioma tissues and cell lines (U87 and U251) by RT-qPCR. Cell proliferation, colony formation, apoptosis, cell cycle, and transwell assays were performed in glioma cell lines transfected with miR-320d mimics or controls to evaluate the effects of miR-320d in vitro. The expression levels of invasive-related proteins were determined by Western blot analysis. Results showed that the expression of miR-320d was significantly decreased in glioma tissues and cell lines. Overexpression of miR-320d could significantly suppress cell growth, migration and invasion, and induced cell apoptosis as well as cell cycle at G0/G1 arrest in U87 and U251 cell lines. Additionally, expression levels of MMP-2, MMP-9, N-cadherin, and integrin-β1 reduced, while E-cadherin increased in miR-320d mimic group. Overall, this study is the first to demonstrate that miR-320d may serve as an independent prognostic factor, indicating that miR-320d is a biomarker for prognosis and therapy in glioma.

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“…Previous studies have reported the function of miR-320d in glioma, colorectal adenoma and B-cell lymphoma. 11,12,30 In the present study, we rst investigated the clinical signicance of miR-320d and explored its role in cell growth, apoptosis, migration and invasion of breast cancer. The results demonstrated that the expression of miR-320d in breast cancer tissues was signi-cantly lower than that in normal tissues.…”

Section: 29mentioning

confidence: 99%

MiR-320d suppresses the progression of breast cancervialncRNA HNF1A-AS1 regulation and SOX4 inhibition

Shi

et al. 2018

RSC Adv.

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MicroRNA-320d (miR-320d) is a novel cancer-related miRNA and functions as a tumor suppressor in human cancers. However, the expression pattern and function of miR-320d in breast cancer remain largely unknown. In the present study, we found that the expression level of miR-320d in breast cancer tissues and cells was significantly lower than in non-tumor tissues and MCF-10A cells. Decreased miR320d was associated with poor overall survival in patients with breast cancer. Overexpression of miR320d inhibited proliferation, migration, and invasion and promoted apoptosis of breast cancer cells. In addition, the long non-coding RNA, HNF1A antisense RNA 1 (HNF1A-AS1) was up-regulated in both breast cancer tissues and cell lines. HNF1A-AS1 suppressed the expression and function of miR-320d.Moreover, SRY-related HMG-box 4 (SOX4) was speculated and confirmed as a target of miR-320d. We also demonstrated that HNF1A-AS1 may function as a sponge competitive endogenous RNA for miR320d, and thus regulate the expression of SOX4. Taken together, our study has identified a novel signaling pathway through which miR-320d exerts its anti-carcinogenic roles and suggested that the HNF1A-AS1/miR-320d/SOX4 may be a potential target for the therapy of breast cancer.

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MicroRNA-320 family is downregulated in colorectal adenoma and affects tumor proliferation by targeting CDK6

Abstract: MiR-320 family affects colorectal tumor proliferation by targeting CDK6, plays important role in its growth, and is considered to be a biomarker for its early detection.

Cited by 60 publications

References 31 publications

Low expression of microRNA-320b correlates with tumorigenesis and unfavorable prognosis in glioma

Low expression of microRNA-320b correlates with tumorigenesis and unfavorable prognosis in glioma

Downregulation of MicroRNA‐320d predicts poor overall survival and promotes the growth and invasive abilities in glioma

MiR-320d suppresses the progression of breast cancervialncRNA HNF1A-AS1 regulation and SOX4 inhibition

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