microRNA-32 inhibits the proliferation and invasion of the SGC-7901 gastric cancer cell line in vitro

Abstract

microRNAs (miRNAs) are a class of endogenously expressed, small non-coding RNAs, which suppress their target mRNAs at the post-transcriptional level. miRNAs play key roles in tumor metastasis. The aim of the present study was to investigate the expression of miRNA-32 (miR-32) on the biological behavior of the human gastric cancer cell line, SGC-7901. SGC-7901 cells were transfected with miR-32-mimic, miR-32-inhibitor and empty plasmid vectors using Lipofectamine™ 2000. The expression of GFP was observed by fluorescent microscopy and miR-32 gene expression was detected by quantitative polymerase chain reaction. The cell counting kit-8 assay was performed to evaluate the effect of miR-32 expression on cell proliferation in vitro. Alterations in the migration and metastatic potential of SGC-7901 cells, prior to and following miR-32 gene transfection, were assayed by cell chemotactic migration and invasion tests. The results of the current study showed that the proliferation rate of the transfected SGC-7901 cells overexpressing miR-32 is reduced and cell chemotactic migration and invasion potentials is markedly reduced following miR-32-mimic transfection (P<0.05). In addition, the results demonstrated that overexpression of miR-32 greatly inhibits the proliferation and decreases the migration and invasion capabilities of SGC-7901 cells in vitro.

MiR-32 induces cell proliferation, migration, and invasion in hepatocellular carcinoma by targeting PTEN

“…Previous studies have demonstrated that certain miRNAs are correlated with the proliferation and survival of HCC, including miR-7, miR-124, miR-122, miR-26a, and miR-199 [10][11][12][13]. Notably, previous studies have demonstrated that overexpression of miR-32 greatly inhibits the proliferation and decreases the migration and invasion capabilities of SGC-7901 cells in vitro [14]. Additionally, it has been reported that miR-32 was significantly down-regulated in osteosarcoma tissues, compared with the adjacent normal tissues, and it could inhibit osteosarcoma cell proliferation and invasion by targeting Sox9 [15].…”

Downregulation of HNF1 homeobox B is associated with drug resistance in ovarian cancer

“…Similarly, miR-375 is associated with drug resistance in ovarian (71) and cervical cancer (72). Although no study has reported the role of miR-32 and -217 in drug resistance, it is associated with drug resistance-related processes such as cell proliferation, invasion and migration (73)(74)(75). Collectively, among the 7 microRNAs most strongly targeting HNF1B, the majority were involved in drug resistance in ovarian and other types of cancers, suggesting that the gene also mediates drug resistance.…”

MiR-32 contributed to cell proliferation of human breast cancer cells by suppressing of PHLPP2 expression

“…MiR-96 promoted cell proliferation of breast cancer by targeting RECK [14]. Herein, we focused on miR-32, which acted as either a tumor suppressor or a tumor promoter in different types of human cancers [15,16]. In this study, we found that miR-32 expression was markedly upregulated in breast cancer tissues and cells.…”