microRNA-31 regulates skeletogenesis by direct suppression of Eve and Wnt1

Sampilo, Nina Faye; Stepicheva, Nadezda A.; Song, Jia L.

doi:10.1016/j.ydbio.2021.01.008

Cited by 9 publications

(21 citation statements)

References 82 publications

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“…Student’s t-test. (B) The percentage of “ingressed, round” PMCs from each embryo were calculated from the total of VegfR10 -expressing PMCs (Sampilo et al, 2021). 3 biological replicates.…”

Section: Resultsmentioning

confidence: 99%

“…(B) miR-1 directly suppresses transcripts (orange) such as Ets1/2, Tbr and Notch which are downstream of miR-31 target , Pmar1 (purple). Both miR-1 and miR-31 directly suppress VegfR7 and Wnt1 (Stepicheva and Song, 2015; Sampilo et al, 2021). miR-1 and miR-31 are critical for proper PMC development.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we identified that miR-31 in the sea urchin regulates skeletogenesis by directly suppressing Eve and Wnt1 (Sampilo et al, 2021). Depletion of miR-31 resulted in expanded vegetal spatial expression of Vegf3 (Stepicheva and Song, 2015; Sampilo et al, 2021), similar to miR-1 mimic-injections. miR-1 directly suppresses genes Ets1/2, Tbr, VegfR7 and Wnt1, of which Ets1/2 and Tbr are downstream of miR-31 targets Pmar1 and Eve (Stepicheva and Song, 2015; Sampilo et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…FoxA, Krl, Vegf3 and Wnt1 RNA probes were previously cloned Sampilo et al, 2021). WMISH was conducted according to previous publications (Arenas-Mena et al, 2000;Minokawa et al, 2004;.…”

Section: Whole Mount In Situ Hybridization (Wmish)mentioning

confidence: 99%

“…1A) (Logan et al, 1999; Martik et al, 2016). By the mesenchyme blastula stage (24 hours post fertilization; hpf), germ layer specification has already occurred;during gastrulation, the three germ layers are differentiated by cross-regulation between signaling pathways, GRN interactions (Lyons et al, 2012), as well as post-transcriptional regulation mediated by miRNAs (Stepicheva and Song, 2015; Sampilo et al, 2018; Sampilo et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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microRNA-1 represses signaling pathway components to impact embryonic structures derived from all three germ layers

Sampilo

Song

2022

Preprint

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microRNAs are evolutionarily conserved non-coding RNAs that direct post-transcriptional regulation of target transcripts. We use the sea urchin embryo to achieve a comprehensive understanding the function of miR-1 in a developing embryo. Results indicate that miR-1 regulates gut contractions, specification, and positioning of serotonergic neurons, as well as mesodermally-derived muscles, pigment cells, and skeletogenic cells. Gain-of-function of miR-1 generally leads to more severe developmental defects than its loss-of-function. We identified that miR-1 directly suppresses Ets1/2, Tbr, and VegfR7 of the skeletogenic gene regulatory network, and Notch, Nodal, and Wnt1 signaling components. We found that miR-1-mediated direct suppression of Nodal may indirectly regulate FoxQ2 to impact serotonergic neurons. Excess miR-1 may lead to decreased Nodal and Notch that result in decreased circumpharnygeal muscle fibers and the number of pigment cells. The striking ectopic skeletal branching induced by miR-1 mimic injections may be explained by miR-1-mediated direct suppression of Nodal that leads to expression changes of Vegf3, and Fgfa that mediate skeletogenesis. This work demonstrates that miR-1 plays a diverse regulatory role that impacts tissues derived from all germ layers.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…FoxA, Krl, Vegf3 and Wnt1 RNA probes were previously cloned Sampilo et al, 2021). WMISH was conducted according to previous publications (Arenas-Mena et al, 2000;Minokawa et al, 2004;.…”

Section: Whole Mount In Situ Hybridization (Wmish)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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microRNA-1 represses signaling pathway components to impact embryonic structures derived from all three germ layers

Sampilo

Song

2022

Preprint

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Distinct regulatory states control the elongation of individual skeletal rods in the sea urchin embryo

Tarsis

Gildor

Morgulis

et al. 2022

Developmental Dynamics

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Background: Understanding how gene regulatory networks (GRNs) control developmental progression is a key to the mechanistic understanding of morphogenesis. The sea urchin larval skeletogenesis provides an excellent platform to tackle this question. In the early stages of sea urchin skeletogenesis, skeletogenic genes are uniformly expressed in the skeletogenic lineage. Yet, during skeletal elongation, skeletogenic genes are expressed in distinct spatial sub-domains. The regulation of differential gene expression during late skeletogenesis is not well understood.Results: Here we reveal the dynamic expression of the skeletogenic regulatory genes that define a specific regulatory state for each pair of skeletal rods, in the sea urchin Paracentrotus lividus. The vascular endothelial growth factor (VEGF) signaling, essential for skeleton formation, specifically controls the migration of cells that form the postoral and distal anterolateral skeletogenic rods. VEGF signaling also controls the expression of regulatory genes in cells

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microRNA-124 directly suppresses Nodal and Notch to regulate mesodermal development

Konrad,

Arnott,

Testa

et al. 2023

Developmental Biology

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microRNA-31 regulates skeletogenesis by direct suppression of Eve and Wnt1

Cited by 9 publications

References 82 publications

microRNA-1 represses signaling pathway components to impact embryonic structures derived from all three germ layers

microRNA-1 represses signaling pathway components to impact embryonic structures derived from all three germ layers

Distinct regulatory states control the elongation of individual skeletal rods in the sea urchin embryo

microRNA-124 directly suppresses Nodal and Notch to regulate mesodermal development

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