MicroRNA-31/184 is involved in transforming growth factor-β-induced apoptosis in A549 human alveolar adenocarcinoma cells

Wang, Cong-Jie; Li, Bingbing; Tan, Yongwen; Zhang, Guimin; Cheng, Guoliang; Ren, Yushan

doi:10.1016/j.lfs.2019.117205

Cited by 13 publications

(17 citation statements)

References 60 publications

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“…miR-184 was also reported to play a protective role in lung tissue 38 . Using microRNA expression array among 25 healthy and IPF patients, miR-184 expression levels was down-regulated in IPF patient tissues.…”

Section: Discussionmentioning

confidence: 99%

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Circulating miRNA Profiles Associated with Lung Function Decline

Cheng

Xiao

et al. 2021

Preprint

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Background: Lung function decline and miRNAs are known to involve in the pathogenesis process of respiratory diseases. However, the association between miRNA profiles and lung function decline was rarely elucidated. This study aimed to compare the expression pattern of plasma miRNAs among people with rapid lung function decline and healthy controls. Methods: Ten controls and 10 cases with rapid lung function decline in last 3 years were enrolled from the Wuhan-Zhuhai cohort. The miRNA sequencing and bioinformatics analysis were carried out to explore miRNAs expression and possible signal pathways. Pearson correlation analysis was carried out to assess the associations between lung function and miRNAs expression levels.Results: A total of 1209 detected miRNAs were differently expression in the reaseach participants. Among them, 17 miRNAs (miR-6749-5p, miR-6797-3p, miR-4468, miR-4301, miR-629-3p, miR-4713-3p, miR-486-3p, miR-450a-1-3p, miR-4732-5p, miR-514a-5p, miR-193b-5p, miR-6749-5p, miR-3168, miR-4691-5p, miR-6730-5p, miR-184, miR-486-5p) were significantly down-regulated in cases with reduced lung function. The expression levels of these miRNAs, except miR-514a-5p and miR-3168, were significantly correlated with lung function parameters. Through bioinformatics analysis, the Wnt signaling pathway, FoxO signaling pathway, MAPK signaling pathway, Pathway in cancer, inflammatory mediator regulation of TRP channels, and TGF-β signaling pathway maybe involved in mediating the association between 17 identified miRNAs and lung function decline.Conclusions: The plasma miRNA profile of people with rapid lung function decline are different when compared with healthy controls. These miRNAs might become research candidates or biomarkers in the early progression of respiratory diseases.

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Section: Discussionmentioning

confidence: 99%

“…Further in vitro study investigated that miR-184 could bind to the smad2-and akt-3'UTRs and repress the downstream signaling pathways triggered by TGF-β 38 . However, Cristina and colleagues reported contradictory results that miR-184 acted a downstream effector of albuminuria through LPP3 to promote tubulointerstitial brosis 39 .…”

Section: Discussionmentioning

confidence: 99%

Circulating miRNA Profiles Associated with Lung Function Decline

Cheng

Xiao

et al. 2021

Preprint

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“…LncRNAs are also able to act in NSCLC through the TGF-β pathway and integrated MAPK/ERK and PI3K/AKT signaling [43][44]. We identi ed several miRNAs that might integrate TGF-β signaling components through lncRNAs regulated by phenanthriplatin (Fig.…”

Section: Discussionmentioning

confidence: 99%

Cisplatin and phenanthriplatin modulate long-noncoding RNA expression in A549 and IMR90 cells revealing regulation of microRNAs, Wnt/β-catenin and TGF-β signaling

Monroe

Moolani

Irihamye

et al. 2021

Preprint

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The monofunctional platinum(II) complex, phenanthriplatin, acts by blocking transcription, but its regulatory effects on long-noncoding RNAs (lncRNAs) have not been elucidated relative to traditional platinum-based chemotherapeutics, e.g., cisplatin. Here, we treated A549 non-small cell lung cancer and IMR90 lung fibroblast cells for 24 hours with either cisplatin, phenanthriplatin or a solvent control, and then performed microarray analysis to identify regulated lncRNAs. RNA22 v2 microRNA software was subsequently used to identify microRNAs (miRNAs) that might be suppressed by the most regulated lncRNAs. We found that miR-25-5p, -30a-3p, -138-5p, -149-3p, -185-5p, -378j, -608, -650, -708-5p, -1253, -1254, -4458, and − 4516, were predicted to target the cisplatin upregulated lncRNAs, IMMP2L-1, CBR3-1 and ATAD2B-5, and the phenanthriplatin downregulated lncRNAs, AGO2-1, COX7A1-2 and SLC26A3-1. The signaling pathways associated with these miRNAs suggests that phenanthriplatin may modulate Wnt/β-catenin and TGF-β signaling through the MAPK/ERK and PTEN/AKT pathways differently than cisplatin. Further, as some of these miRNAs may be subject to dissimilar lncRNA targeting in A549 and IMR90 cells, the monofunctional complex may not cause toxicity in normal lung compared to cancer cells by acting through distinct lncRNA and miRNA networks.

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“…LncRNAs are also able to act in NSCLC through the TGF-β pathway and integrated MAPK/ERK and PI3K/AKT signaling 43 , 44 . We identified several miRNAs that might integrate TGF-β signaling components through lncRNAs regulated by phenanthriplatin (Fig.…”

Section: Discussionmentioning

confidence: 99%

Cisplatin and phenanthriplatin modulate long-noncoding RNA expression in A549 and IMR90 cells revealing regulation of microRNAs, Wnt/β-catenin and TGF-β signaling

Monroe

Moolani

Irihamye

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The monofunctional platinum(II) complex, phenanthriplatin, acts by blocking transcription, but its regulatory effects on long-noncoding RNAs (lncRNAs) have not been elucidated relative to traditional platinum-based chemotherapeutics, e.g., cisplatin. Here, we treated A549 non-small cell lung cancer and IMR90 lung fibroblast cells for 24 h with either cisplatin, phenanthriplatin or a solvent control, and then performed microarray analysis to identify regulated lncRNAs. RNA22 v2 microRNA software was subsequently used to identify microRNAs (miRNAs) that might be suppressed by the most regulated lncRNAs. We found that miR-25-5p, -30a-3p, -138-5p, -149-3p, -185-5p, -378j, -608, -650, -708-5p, -1253, -1254, -4458, and -4516, were predicted to target the cisplatin upregulated lncRNAs, IMMP2L-1, CBR3-1 and ATAD2B-5, and the phenanthriplatin downregulated lncRNAs, AGO2-1, COX7A1-2 and SLC26A3-1. Then, we used qRT-PCR to measure the expression of miR-25-5p, -378j, -4516 (A549) and miR-149-3p, -608, and -4458 (IMR90) to identify distinct signaling effects associated with cisplatin and phenanthriplatin. The signaling pathways associated with these miRNAs suggests that phenanthriplatin may modulate Wnt/β-catenin and TGF-β signaling through the MAPK/ERK and PTEN/AKT pathways differently than cisplatin. Further, as some of these miRNAs may be subject to dissimilar lncRNA targeting in A549 and IMR90 cells, the monofunctional complex may not cause toxicity in normal lung compared to cancer cells by acting through distinct lncRNA and miRNA networks.

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MicroRNA-31/184 is involved in transforming growth factor-β-induced apoptosis in A549 human alveolar adenocarcinoma cells

Cited by 13 publications

References 60 publications

Circulating miRNA Profiles Associated with Lung Function Decline

Circulating miRNA Profiles Associated with Lung Function Decline

Cisplatin and phenanthriplatin modulate long-noncoding RNA expression in A549 and IMR90 cells revealing regulation of microRNAs, Wnt/β-catenin and TGF-β signaling

Cisplatin and phenanthriplatin modulate long-noncoding RNA expression in A549 and IMR90 cells revealing regulation of microRNAs, Wnt/β-catenin and TGF-β signaling

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