MicroRNA-30b Regulates High Phosphorus Level-Induced Autophagy in Vascular Smooth Muscle Cells by Targeting BECN1

Wang, Jian; Sun, Yi-Ting; Xu, Tianhua; Sun, Wei; Tian, Binyao; Sheng, Zitong; Sun, Li; Liu, Linlin; Ma, Jun; Wang, Lining; Li, Yao

doi:10.1159/000477602

Cited by 16 publications

(17 citation statements)

References 25 publications

(31 reference statements)

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“…32 LC3II was considered as an autophagy-linked marker in cell tissues. 33 YAP plays a role in the processes of autophagy and apoptosis 34 and silencing YAP increased the ratio of LC3-I and LC3-II, accompanied by the autophagy-related protein Beclin1. 15 Caspase-3 plays an important role in the caspase cascade reaction and represents a major enzyme and promoter of apoptosis in cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA TNRC6C‐AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway

Peng

Tan

et al. 2019

J Cellular Molecular Medi

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The role of long non‐coding RNAs (lncRNAs) in thyroid carcinoma (TC), the most frequent endocrine malignancy, has been extensively examined. This study investigated effect of interaction among lncRNA TNRC6C‐AS1, serine/threonine‐protein kinase 4 (STK4) and Hippo signalling pathway on TC. Initially, lncRNA TNRC6C‐AS1 expression in TC tissues was detected. To explore roles of lncRNA TNRC6C‐AS1, STK4 and Hippo signalling pathway in TC progression, their expressions were altered. Interaction between lncRNA TNRC6C‐AS1 and STK4, STK4 promoter methylation, or Hippo signalling pathway was verified. After that, a series of experiments were employed to evaluate in vitro ability of apoptosis, proliferation and autophagy of TC cells and in vivo tumorigenicity, and tumour growth of TC cells. lncRNA TNRC6C‐AS1 was highly expressed while STK4 was poorly expressed in TC tissues. LncRNA TNRC6C‐AS1 promoted the STK4 methylation and down‐regulated STK4 expression, which further activated the Hippo signalling pathway. STK4 silencing was observed to promote the proliferation ability of TC cells, inhibit the apoptosis and autophagy abilities, as well as enhance the tumorigenicity and tumour growth. Moreover, the in vitro proliferation ability as well as the in vivo tumorigenicity and tumour growth of TC cells were inhibited after the blockade of Hippo signalling pathway, while the apoptosis and autophagy abilities were promoted. The results demonstrate that the lncRNA TNRC6C‐AS1 increases STK4 promoter methylation to down‐regulate STK4 expression, thereby promoting the development of TC through activation of Hippo signalling pathway. It highlights that lncRNA TNRC6C‐AS1 may be a novel therapeutic target for the treatment of TC.

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Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA TNRC6C‐AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway

Peng

Tan

et al. 2019

J Cellular Molecular Medi

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“…miR-30b has been reported to be downregulated in GC and was shown to inhibit the development of GC by targeting plasminogen activator inhibitor-1 (22). Moreover, miR-30b overexpression inhibited high phosphorus (Pi)-induced autophagy by reducing the expression of autophagy-related genes, such as ATG5, in vascular smooth muscle cells (24). Additionally, miR-30b suppressed autophagy to abate hepatic ischemia-reperfusion injury by reducing ATG12-ATG5 conjugates (23).…”

Section: Discussionmentioning

confidence: 99%

“…Hence, bioinformatics analysis was performed by miRcode online website to identify miRNAs that may interact with MALAT1. Among candidate miRNAs, miR-30b was selected due to its critical role in GC progression and autophagy (22)(23)(24) (Fig. 3A).…”

Section: Malat1 Inhibits Mir-30b Expression By Direct Interactionmentioning

confidence: 99%

LncRNA MALAT1 potentiates autophagy‑associated cisplatin resistance by regulating the microRNA‑30b/autophagy‑related gene 5 axis in gastric cancer

Si²,

Jiang

2018

Int J Oncol

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Gastric cancer (GC) is the fourth most common type of cancer worldwide and chemoresistance is a major obstacle to successful GC treatment. In the present study, reverse transcription-quantitative polymerase chain reaction analysis was used to measure the expression of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and microRNA (miR)-30b. Western blot analysis was conducted to detect the protein expression of autophagy-related gene 5 (ATG5), p62 and LC3 (LC3-I and LC3-II). Cell viability and half maximal inhibitory concentration were determined by the Cell Counting Kit-8 assay. The green fluorescent protein (GFP)-LC3-positive cell percentage was determined by the GFP-LC3 puncta experiment. Luciferase reporter and RNA immunoprecipitation assays were used to explore the molecular associations among MALAT1, miR-30b and ATG5. MALAT1 was found to be highly expressed in CDDP-resistant AGS(AGS/CDDP) cells and CDDP-resistant HGC-27 (HGC-27/CDDP) cells. Cell viability was markedly increased in MALAT1-overexpressing AGS/CDDP cells, but was notably reduced in MALAT1-depleted HGC-27/CDDP cells. Moreover, MALAT1 potentiated CDDP resistance by facilitating autophagy in AGS/CDDP and HGC-27/CDDP cells. Further investigations demonstrated that MALAT1 inhibited miR-30b expression by direct interaction. Moreover, miR-30b abolished MALAT1-induced CDDP resistance by inhibiting autophagy in AGS/CDDP and HGC-27/CDDP cells. Furthermore, ATG5 was found to be a target of miR-30b. miR-30b weakened resistance to CDDP by inhibiting autophagy in AGS/CDDP and HGC-27/CDDP cells, while this effect was abrogated by increased ATG5 expression. Additionally, MALAT1 sequestered miR-30b from ATG5 to increase ATG5 expression in AGS/CDDP and HGC-27/CDDP cells. Therefore, MALAT1 potentiated autophagy-related CDDP resistance through suppressing the miR-30b/ATG5 axis in AGS/CDDP and HGC-27/CDDP cells, indicating that it may represent a promising target for the reversal of chemoresistance in GC.

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“…This eventually inhibits autophagy by down-regulating the expression of LC3II and ATG5, as well as by elevating the expression of SQSTM1/p62 [ 77 ]. MiR-30b disrupts autophagy in vascular smooth muscle cells by decreasing autophagy-related genes, such as Atg5 and LC3II [ 78 ]. MiR-212 and miR-132 inhibit autophagy by negatively regulating the pro-autophagic transcription factor forkhead box O3 (FOXO3).…”

Section: The Role Of Micrornas In Cardiac Autophagymentioning

confidence: 99%

MicroRNAs in Cardiac Autophagy: Small Molecules and Big Role

Sun

et al. 2018

Cells

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Autophagy, which is an evolutionarily conserved process according to the lysosomal degradation of cellular components, plays a critical role in maintaining cell homeostasis. Autophagy and mitochondria autophagy (mitophagy) contribute to the preservation of cardiac homeostasis in physiological settings. However, impaired or excessive autophagy is related to a variety of diseases. Recently, a close link between autophagy and cardiac disorders, including myocardial infarction, cardiac hypertrophy, cardiomyopathy, cardiac fibrosis, and heart failure, has been demonstrated. MicroRNAs (miRNAs) are a class of small non-coding RNAs with a length of approximately 21–22 nucleotides (nt), which are distributed widely in viruses, plants, protists, and animals. They function in mediating the post-transcriptional gene silencing. A growing number of studies have demonstrated that miRNAs regulate cardiac autophagy by suppressing the expression of autophagy-related genes in a targeted manner, which are involved in the pathogenesis of heart diseases. This review summarizes the role of microRNAs in cardiac autophagy and related cardiac disorders. Furthermore, we mainly focused on the autophagy regulation pathways, which consisted of miRNAs and their targeted genes.

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MicroRNA-30b Regulates High Phosphorus Level-Induced Autophagy in Vascular Smooth Muscle Cells by Targeting BECN1

Cited by 16 publications

References 25 publications

Long non‐coding RNA TNRC6C‐AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway

Long non‐coding RNA TNRC6C‐AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway

LncRNA MALAT1 potentiates autophagy‑associated cisplatin resistance by regulating the microRNA‑30b/autophagy‑related gene 5 axis in gastric cancer

MicroRNAs in Cardiac Autophagy: Small Molecules and Big Role

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