MicroRNA-30a regulates zebrafish myogenesis via targeting the transcription factor Six1

O’Brien, Jenean; Hernandez-Lagunas, Laura; Artinger, Kristin; Ford, Heide L.

doi:10.1242/jcs.143677

Cited by 29 publications

(25 citation statements)

References 81 publications

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“…Recently, a strong selective sweep near the vgll3 gene was postulated to be due to energy metabolism effects in humans in Mongolia (Nakayama et al 2017). In turn, genes in the six6 genomic region are involved in cell growth, cell differentiation, apoptosis in human cell lines ( PPM1A , Lin et al 2006), and myogenesis and skeletal muscle cell proliferation in zebrafish ( six1b , Ridgeway and Skerjanc 2001, Bessarab et al 2004, O’Brien et al 2014) and act as an evolutionarily conserved regulator of eye development and the pituitary–hypothalamic axis ( six6 , Serikaku and Otousa 1994, Toy et al 1998, Gallardo et al 1999). Collectively, this suggests that the vgll3 and six6 haploblocks might have broad-scale roles in reproductive and life-history strategies in Atlantic salmon.…”

Section: Introductionmentioning

confidence: 99%

Life history genomic regions explain differences in Atlantic salmon marine diet specialization

Aykanat

Rasmussen

Ozerov

et al. 2019

Preprint

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9An ecological consequence of climate change is the alteration of food-web structures. Species with 10 ontogenetic (age-dependent) diet variation, such as Atlantic salmon (Salmo salar), may exhibit an age-11 dependent response to food-web perturbations, which may subsequently influence the demographic structure. 12 We previously showed that age at maturity in Atlantic salmon is primarily influenced by few genomic regions 13 (vgll3 and six6), but whether these regions are linked to diet is unknown. We hypothesized that genetic 14 variation in these life history genomic regions govern age-dependent resource utilization efficiency, which 15 would subsequently influence age at maturity. To test this, we first performed stomach content analysis of 16 Atlantic salmon sampled at sea on their return migration to fresh water, followed by targeted genotyping by 17 sequencing. Here, we first showed that Atlantic salmon change their feeding strategies along their ontogeny. 18Consistent with the so-called feast and famine strategy, older age groups retained a heavier stomach content, 19 which however came at the expense of running on empty more often. Next, we presented evidence that 20 stomach fullness in Atlantic salmon is associated with six6, a gene previously shown to be potentially under 21 divergent selection and correlated with age at maturity among populations. There was no association with 22 vgll3, a gene with a large effect on sea age at maturity. Prey composition was marginally linked to both six6 23 and vgll3. Our results suggest that Atlantic salmon individuals are not as generalist as previously thought and 24 that genetic variation partly underlies resource utilization variation among individuals. Given that feeding 25 strategies differ ontogenetically, and a spatially divergent genomic region is associated with diet acquisition 26 variation, we predict populations with diverse maturation age will have diverse evolutionary responses to 27 future changes in marine food-web structures.

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Section: Introductionmentioning

confidence: 99%

Life history genomic regions explain differences in Atlantic salmon marine diet specialization

Aykanat

Rasmussen

Ozerov

et al. 2019

Preprint

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“…In zebrafish, the Six1a ortholog drives proliferation and differentiation of zebrafish embryonic muscle fiber precursor cells, and its knockdown results in apoptosis (O'Brien et al, 2014). Six1 also plays a key role in the transcriptional regulation of the myogenic regulatory factor (MRF) gene family (Fougerousse et al, 2002; Grifone et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

LncRNA-Six1 Encodes a Micropeptide to Activate Six1 in Cis and Is Involved in Cell Proliferation and Muscle Growth

Cai

et al. 2017

Front. Physiol.

110

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Long non-coding RNAs (lncRNAs) play important roles in epigenetic regulation of skeletal muscle development. In our previous RNA-seq study (accession number GSE58755), we found that lncRNA-Six1 is an lncRNA that is differentially expressed between White Recessive Rock (WRR) and Xinghua (XH) chicken. In this study, we have further demonstrated that lncRNA-Six1 is located 432 bp upstream of the gene encoding the protein Six homeobox 1 (Six1). A dual-luciferase reporter assay identified that lncRNA-Six1 overlaps the Six1 proximal promoter. In lncRNA-Six1, a micropeptide of about 7.26 kDa was found to play an important role in the lncRNA-Six1 in cis activity. Overexpression of lncRNA-Six1 promoted the mRNA and protein expression level of the Six1 gene, while knockdown of lncRNA-Six1 inhibited Six1 expression. Moreover, tissue expression profiles showed that both the lncRNA-Six1 and the Six1 mRNA were highly expressed in chicken breast tissue. LncRNA-Six1 overexpression promoted cell proliferation and induced cell division. Conversely, its loss of function inhibited cell proliferation and reduced cell viability. Similar effects were observed after overexpression or knockdown of the Six1 gene. In addition, overexpression or knockdown of Six1 promoted or inhibited, respectively, the expression levels of muscle-growth-related genes, such as MYOG, MYHC, MYOD, IGF1R, and INSR. Taken together, these data demonstrate that lncRNA-Six1 carries out cis-acting regulation of the protein-encoding Six1 gene, and encodes a micropeptide to activate Six1 gene, thus promoting cell proliferation and being involved in muscle growth.

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“…There are MEF3 sites in the core area of the MyoG enhancer, and when Six1 combines with MEF3, MyoG is expressed (Zhang et al, 2009). In zebrafish, more than half of the embryos were MyoG negative cells when Six1a expression was blocked, and the process of rapid muscle differentiation was inhibited (O'Brien et al, 2014). Previous studies have suggested that over expression of Six1 up-regulates MyoG expression and over expression of Six4 down-regulates MyoG expression (Yajima et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Research Article Association of <i>Six1</i> and <i>Six4</i> gene expression with meat tenderness in Tan sheep

Xu¹,

Ma²,

Molaaimaiti³

et al. 2018

Genet. Mol. Res.

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Sine oculis-related homeobox 1 (Six1) and 4 (Six4) transcription factors are expressed in developing and adult muscle. These Six homeoproteins are required to activate the fast-type muscle program in the mouse primary myotome. Whether Six1 and Six4 genes have a role in meat tenderness in Tan sheep has not yet been established. To study the characteristics and mechanisms of meat tenderness in Tan sheep, we sampled the musculus triceps muscle, the longest neck muscles, abdominal muscles, gluteus and biceps femoris muscle and measured Warner-Bratzler shear force and texture profile analysis using a texture analyzer (TMS-Pro), and mRNA expression of Six1 and Six4by real-time PCR, and we performed a correlation analysis. The Warner-Bratzler shear force was significantly different among the five different muscles, but there was no difference in this measure between the triceps and biceps femoris muscles. Hardness, gumminess and chewiness were significantly different among the different muscles. Six1 and Six4 mRNA expression was significantly higher in the longest neck muscles than in the other muscles. There was a significant negative correlation between Six1 mRNA expression and the texture parameters gumminess and chewiness, and a significant positive correlation between Six4 mRNA expression and gumminess and chewiness. Based on these results, we suggest that Six1 and Six4 not only regulate muscle fiber typing, but also meat tenderness in Tan sheep. They could thus be candidate genes for selecting for meat tenderness.

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MicroRNA-30a regulates zebrafish myogenesis via targeting the transcription factor Six1

Cited by 29 publications

References 81 publications

Life history genomic regions explain differences in Atlantic salmon marine diet specialization

Life history genomic regions explain differences in Atlantic salmon marine diet specialization

LncRNA-Six1 Encodes a Micropeptide to Activate Six1 in Cis and Is Involved in Cell Proliferation and Muscle Growth

Research Article Association of <i>Six1</i> and <i>Six4</i> gene expression with meat tenderness in Tan sheep

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