MicroRNA-30a Inhibits Proliferation and Metastasis of Osteosarcoma Cells by Modulating Autophagy

Yuan, Tongzhou; Wang, Juan; Wei, Xincheng; Wang, Guodong; Xu, Nuo; Fan, Lei

doi:10.1166/jbmb.2016.1588

Cited by 3 publications

(1 citation statement)

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“…6 A previous study based on gene expression profiling has shown that seven miRNAs are associated with OS, including miR-422a, -145, -194, -93, -125b, -193a-3p, and -140-5p. 7 Some miRNAs have been proved to be downregulated in OS, such as miR-30a, 8 -33b, 9 -101, 10 -379, 11 -493-5p, 12 -567, 13 and -638. 14 These miRNAs exert valuable anti-tumor effect on OS through regulating diverse cellular processes.…”

Section: Introductionmentioning

confidence: 99%

<p>MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3</p>

Sun¹,

Fu²,

Wang³

et al. 2020

CMAR

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Purpose This study aimed to evaluate the regulatory role of miR-431-5p on the tumorigenesis of osteosarcoma (OS) and the underlying mechanism involving pannexin 3 ( PANX3 ). Methods qRT-PCR was applied to measure the expression of miR-431-5p in OS tissues and cells. PANX3 and miR-431-5p were overexpressed in U2OS and HOS cells. The cell viability and apoptosis were determined by MTT and FITC/PI double staining assay, respectively. Transwell assay was performed to detect cell migration and invasion. The protein expression of cleave-caspase-3 and MMP-2/-9 was detected by Western blot. The target relationship between miR-431-5p and PANX3 was predicated by ENCORI and identified by DLR assay. The anti-tumor effect of miR-431-5p was further analyzed in a xenograft tumor model in mice. Results MiR-431-5p expression was down-regulated in OS tissues and negatively correlated with lymph node metastasis and TNM stage. Over-expression of miR-431-5p induced cell apoptosis, inhibited cell proliferation, migration and invasion, up-regulated cleave-caspase-3, and down-regulated MMP-2 and -9 in OS cells. Over-expression of miR-431-5p also inhibited the growth of tumor xenografts in mice. In addition, PANX3 was a target of miR-431-5p. Over-expression of PANX3 reversed the anti-tumor effect of miR-431-5p mimics on U2OS and HOS cells. Conclusion Up-regulation of miR-431-5p suppressed the tumorigenesis of OS via targeting PANX3 .

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Section: Introductionmentioning

confidence: 99%

<p>MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3</p>

Sun¹,

Fu²,

Wang³

et al. 2020

CMAR

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ac4C acetylation regulates mRNA stability and translation efficiency in osteosarcoma

Zhang,

Gao,

Fan

et al. 2023

Heliyon

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Mechanism for Bioactive Nanomaterial circ0024831 Regulation of Staphylococcal Nuclease Domain Containing 1 via RNA Methylation Recognition in Osteosarcoma

Zheng

Wang

Zhang

et al. 2022

j biomed nanotechnol

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Bioactive nanomaterial circular RNA (circRNA) is an important non-coding RNA with a strong specificity, stable structure and high expression abundance. It can affect many diseases and physiological processes and may become a new way of disease diagnosis and targeted therapy. Recent studies have shown that Staphylococcal Nuclease Domain-Containing Protein 1 (SND1) can recognize N6-methyladenine (M6A) modified mRNA and regulate target mRNA stability. It can then control the expression of a series of downstream genes. However, whether SND1 can directly combine with circRNA and regulate its stability and function are new issues to be discussed. Results showed bioactive nanomaterial circ0024831 could directly bind to the Tudor domain of SND1 in the cytoplasm to block the recognition of SND1 to M6A modified RNA thus reducing the stability of downstream target gene mRNA and inhibiting the expression of downstream regulatory proteins. The down-regulation of circ0024831 expression in osteosarcoma cells relieved inhibition of SND1 which lead to change of tumor-related gene expression profile, promoting the occurrence and development of osteosarcoma.

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MicroRNA-30a Inhibits Proliferation and Metastasis of Osteosarcoma Cells by Modulating Autophagy

Cited by 3 publications

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<p>MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3</p>

<p>MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3</p>

ac4C acetylation regulates mRNA stability and translation efficiency in osteosarcoma

Mechanism for Bioactive Nanomaterial circ0024831 Regulation of Staphylococcal Nuclease Domain Containing 1 via RNA Methylation Recognition in Osteosarcoma

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