MicroRNA-301b-3p accelerates the growth of gastric cancer cells by targeting zinc finger and BTB domain containing 4

Fan, Hui; Jin, Xianzhen; Liao, Chunyan; Qiao, Liang; Zhao, Wei

doi:10.1016/j.prp.2019.152667

Cited by 32 publications

(31 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it has been found to be downregulated in several malignancies, functioning as a tumor suppressor in hepatocellular carcinoma (HCC), 20 malignant glioma, 21 as well as being associated with poor prognosis and facilitating tumor cell growth in breast cancer 22,23 . It has previously been reported that miR‐301b‐3p serves as a tumor promoter which drives the growth of gastric cancer (GC) 24 and HCC 25 . It also facilitates tumor metastasis in high‐grade ovarian serous cancer by targeting CPEB3/EGFR axis 26 .…”

Section: Discussionmentioning

confidence: 99%

Plasma miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p as potential biomarkers for early diagnosis of non‐small cell lung cancer

et al. 2020

View full text Add to dashboard Cite

Background Accumulating evidence shows that microRNAs are aberrantly expressed and exert essential roles in the tumorigenesis and tumor progression of non‐small cell lung cancer (NSCLC). Methods The plasma miRNAs from five healthy donors and four NSCLC patients were profiled by miRNA microarray. The differentially expressed miRNAs from 154 primary NSCLC patients and 146 healthy donors were subjected to RNA isolation and verified by quantitative PCR (qPCR). Results The miRNA microarray analysis revealed that 40 differential miRNAs between NSCLC patients and healthy donors were selected. We found that the plasma miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p levels of patients were significantly higher than those of healthy controls. The receiver operating characteristic curve (ROC) analyses revealed higher area under the ROC curve (AUC) values and higher sensitivity/specificity of carcinoembryonic antigen (CEA) in combination with miR‐1247‐5p, miR‐301b‐3p, or miR‐105‐5p were superior to that of CEA alone. Conclusions High miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p expression have been demonstrated to accelerate tumorigenesis, and these three miRNAs in plasma act as novel biomarkers for the early diagnosis of NSCLC patients. Key points Plasma miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p act as novel biomarkers for early NSCLC and NSCLC.

show abstract

Section: Discussionmentioning

confidence: 99%

Plasma miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p as potential biomarkers for early diagnosis of non‐small cell lung cancer

et al. 2020

View full text Add to dashboard Cite

show abstract

“…It has been reported miR‐301b was involved in the tumorigenesis of several cancers. A recent paper reported miR‐301b was upregulated in gastric cancer (GC), and knockdown of miR‐301b inhibited GC cell proliferation, and induced cell cycle arrest and apoptosis 17 . It also revealed ZBTB4 was a target of miR‐301b, and miR‐301b overexpression reduced its expression in GC cells 17 .…”

Section: Discussionmentioning

confidence: 99%

“…A recent paper reported miR‐301b was upregulated in gastric cancer (GC), and knockdown of miR‐301b inhibited GC cell proliferation, and induced cell cycle arrest and apoptosis 17 . It also revealed ZBTB4 was a target of miR‐301b, and miR‐301b overexpression reduced its expression in GC cells 17 . In pancreatic carcinoma, it was reported miR‐301b overexpression suppressed TP63 expression and contributed to promote cell invasiveness and enhance gemcitabine resistance in pancreatic carcinoma cells 18 .…”

Section: Discussionmentioning

confidence: 99%

Inhibiting microRNA‐301b suppresses cell growth and targets RNF38 in cervical carcinoma

Guo

et al. 2020

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

It has been reported microRNA‐301b (miR‐301b) was involved in the tumorigenesis of some cancers, but it has not been investigated in cervical carcinoma yet. In this study, miR‐301b was found significantly upregulated in cervical carcinoma, and patients with high miR‐301b expression had a shorter overall survival. When miR‐301b was knocked down in cervical carcinoma cells, the cell growth could be significantly abolished. Our further studies showed miR‐301b targeted RNF38, and inhibited its expression in cervical carcinoma cells. Moreover, RNF38 was found downregulated in cervical carcinoma, and miR‐301b expression in cervical tissues was found negatively correlated with RNF38 expression. In addition, overexpression of RNF38 significantly inhibited cervical carcinoma cell growth, but overexpression of miR‐301b suppressed RNF38‐induced cell growth inhibition in cervical carcinoma. Collectively, this study suggested miR‐301b could be a novel target for cervical carcinoma treatment.

show abstract

“…Optical-density values at 450 nm were measured with a microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). The Cell-Light™ EdU Apollo®488 In vitro Imaging Kit (RIBOBIO, Guangzhou, China) was used for EdU staining, as previously described [23].…”

Section: Cell Proliferation Analysismentioning

confidence: 99%

Long non-coding RNA MSC-AS1 facilitates the proliferation and glycolysis of gastric cancer cells by regulating PFKFB3 expression

Jin¹,

Qiao²,

Fan³

et al. 2021

Int. J. Med. Sci.

Self Cite

View full text Add to dashboard Cite

Long non-coding RNA musculin antisense RNA 1 (lncRNA MSC-AS1) has been recognized as an oncogene in pancreatic cancer, hepatocellular carcinoma, nasopharyngeal carcinoma, and renal cell carcinoma. However, the functional significance of MSC-AS1 and its underlying mechanism in gastric cancer (GC) progression remain unclear. In this study, we demonstrated that the expression of MSC-AS1 in GC tissues was significantly higher than that in non-tumor tissues. Moreover, the elevated level of MSC-AS1 was detected in GC cells (MKN-45, AGS, SGC-7901, and MGC-803) compared to normal GES-1 gastric mucosal cells. The cancer genome atlas (TCGA) data further indicated that the high level of MSC-AS1 was closely correlated with advanced tumor stage and poor prognosis of GC. Next, we revealed that MSC-AS1 knockdown inhibited the proliferation, glucose consumption, lactate production, and pyruvate production of MGC-803 cells. Conversely, MSC-AS1 overexpression enhanced the proliferation and glycolysis of AGC cells. Mechanistically, modulating MSC-AS1 level affected the expression of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), but did not impact the levels of hexokinase 2 (HK2) and pyruvate kinase M2 (PKM2) in GC cells. Based on this, we reversed the MSC-AS1 knockdown-induced the inhibition of cell proliferation and glycolysis by restoring PFKFB3 expression in MGC-803 cells. In conclusion, MSC-AS1 facilitated the proliferation and glycolysis of GC cells by maintaining PFKFB3 expression.

show abstract

MicroRNA-301b-3p accelerates the growth of gastric cancer cells by targeting zinc finger and BTB domain containing 4

Cited by 32 publications

References 29 publications

Plasma miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p as potential biomarkers for early diagnosis of non‐small cell lung cancer

Plasma miR‐1247‐5p, miR‐301b‐3p and miR‐105‐5p as potential biomarkers for early diagnosis of non‐small cell lung cancer

Inhibiting microRNA‐301b suppresses cell growth and targets RNF38 in cervical carcinoma

Long non-coding RNA MSC-AS1 facilitates the proliferation and glycolysis of gastric cancer cells by regulating PFKFB3 expression

Contact Info

Product

Resources

About