MicroRNA‐29a suppresses the growth, migration, and invasion of lung adenocarcinoma cells by targeting carcinoembryonic antigen‐related cell adhesion molecule 6

Han, Hye Sook; Son, Seung-Myoung; Yun, Jieun; Jo, Yeong Nang; Lee, Ok-Jun

doi:10.1016/j.febslet.2014.08.023

Cited by 27 publications

(26 citation statements)

References 39 publications

(64 reference statements)

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“…In addition, the roles of miRNAs in LA metastasis are also reported [31]. Some miRNAs were reported to promote LA metastasis [32, 33], while other miRNAs were reported to inhibit LA metastasis [34, 35]. These data clearly suggest that dysregulation of miRNAs is involved in tumorigenesis and development of LA.…”

Section: Discussionmentioning

confidence: 95%

Prognostic Significance of microRNA-7 and its Roles in the Regulation of Cisplatin Resistance in Lung Adenocarcinoma

Cheng

Shen

et al. 2017

Cell Physiol Biochem

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Background: Previously, microRNA (miR)-7 has been reported to function as a tumor suppressor in human cancers, but the correlations of miR-7 expression with prognosis and cisplatin (CDDP) resistance in lung adenocarcinoma (LA) are unclear. Here, our aim is to determine the prognostic significance of miR-7 and its roles in the regulation of CDDP resistance in LA. Methods: Quantitative real-time PCR (qRT-PCR) assay was performed to determine miR-7 expression in 108 paired of LA tissues and analyze its correlations with clinicopathological factors of patients. The patient survival data were collected retrospectively by Kaplan-Meier analyses, and multivariate analysis was performed using the Cox proportional hazards model to determine the prognostic significance of miR-7 expression. The effects of miR-7 expression on the chemosensitivity of LA cells to CDDP and its possible mechanisms were evaluated by MTT, flow cytometry, Western blot and luciferase assays. Results: It was observed that the relative expression level of miR-7 in LA tissues was significantly lower than that in the adjacent normal tissues and low miR-7 expression level was closely associated with poorer tumor differentiation, advanced pathological T-factor, higher incidence of lymph node metastasis and advanced p-TNM stage. Also, patients with low miR-7 expression showed a shorter overall survival than those with high miR-7 expression, and multivariate analysis indicated that status of miR-7 expression was an independent molecular biomarker for predicting the overall survival (OS) of LA patients. In addition, upregulation of miR-7 increases the sensitivity of LA cells to CDDP via induction of apoptosis by targeting Bcl-2. Conclusions: Our finding for the first time demonstrates that low miR-7 expression may be an independent poor prognostic factor and targeting miR-7 may be a potential strategy for the reversal of CDDP resistance in LA.

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Section: Discussionmentioning

confidence: 95%

Prognostic Significance of microRNA-7 and its Roles in the Regulation of Cisplatin Resistance in Lung Adenocarcinoma

Cheng

Shen

et al. 2017

Cell Physiol Biochem

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“…[16][17][18][19] Recently, the miR-17-5p has been reported to be associated with tumor progression and metastasis via targeting multiple signaling pathways, such as the Wnt/β-catenin and MAPK signaling pathways. [16][17][18][19] Recently, the miR-17-5p has been reported to be associated with tumor progression and metastasis via targeting multiple signaling pathways, such as the Wnt/β-catenin and MAPK signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

miR‐17‐5p promotes proliferation and epithelial‐mesenchymal transition in human osteosarcoma cells by targeting SRC kinase signaling inhibitor 1

Zhao

Sun

et al. 2018

J of Cellular Biochemistry

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MicroRNA‐17‐5p (miR‐17‐5p) and epithelial‐mesenchymal transition (EMT) have been reported to participate in the development and progression of multiple cancers. However, the relationship between the miR‐17‐5p and EMT in osteosarcoma (OS) is still poorly understood. This study was to investigate the effects of the miR‐17‐5p and its potential mechanism in regulating proliferation, apoptosis, and EMT of human OS. Quantitative real‐time PCR was used to detect the miR‐17‐5p and SRC kinase signaling inhibitor 1 (SRCIN1) messenger RNA expression in OS specimens and cell lines. After transfection with miR‐17‐5p inhibitors, proliferation, apoptosis, migration, and invasion of OS cells were assessed by using the Cell Counting Kit‐8, the annexin V‐FITC apoptosis, wound‐healing, and transwell assays. The SRCIN1 was validated as a target of the miR‐17‐5p through bioinformatics algorithms and luciferase reporter assay. Moreover, the expression of EMT markers, E‐cadherin, N‐cadherin, and Snail was identified by the Western blot analysis. MiR‐17‐5p was significantly upregulated in OS tumor samples and cell lines. It inhibited proliferation and EMT, and promoted apoptosis in OS. The SRCIN1 was identified as a direct target of the miR‐17‐5p. Silenced miR‐17‐5p could change the expression of EMT markers, such as upregulating the expression of E‐cadherin, and downregulating the expression of N‐cadherin and Snail through targeting the antioncogenic SRCIN1. These findings suggest that the miR‐17‐5p promotes cell proliferation, and EMT in human OS by directly targeting the SRCIN1, and reveal a branch of the miR‐17‐5p/SRCIN1/EMT signaling pathway involved in the progression of OS.

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“…miRNAs are increasingly reported to be differentially expressed in various tumors either as oncogenes or tumor suppressors (27–29). In a previous study, miR-145 was identified to target MUC13 inhibiting the tumor growth and invasion in pancreatic tissues.…”

Section: Discussionmentioning

confidence: 99%

Reduction of miR-132-3p contributes to gastric cancer proliferation by targeting MUC13

Liu

Wei

et al. 2017

Molecular Medicine Reports

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Abnormal expression of epidermal growth factor receptor (EGFR) signaling and microRNAs (miRNAs) has been widely seen in gastric cancer. The present study focused on the miRNAs that regulate human epidermal growth factor receptor (HER) activation through mucin 13 (MUC13). The protein level of MUC13 was demonstrated to be significantly increased in gastric cancer tissues compared with normal tissues by western blot analysis and immunohistochemistry. TargetScan bioinformatic predictions indicated that miRNA (miR)-212-3p and miR-132-3p may bind to the 3′-untranslated region of MUC13. Further investigation revealed that miR-132-3p was significantly decreased in gastric cancer tissues compared with normal tissues, whereas miR-212-3p expression was unaffected. Luciferase assays and western blot confirmed MUC13 as a target gene of miR-132-3p. Inhibition of miR-132-3p enhanced gastric cancer cell migration through activation of HER2, extracellular signal-regulated kinase (ERK) and Akt serine/threonine kinase (Akt) signaling, which was a similar effect to that of MUC13 overexpression. In summary, reduction of miR-132-3p may contribute to gastric cancer proliferation by targeting MUC13.

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MicroRNA‐29a suppresses the growth, migration, and invasion of lung adenocarcinoma cells by targeting carcinoembryonic antigen‐related cell adhesion molecule 6

Cited by 27 publications

References 39 publications

Prognostic Significance of microRNA-7 and its Roles in the Regulation of Cisplatin Resistance in Lung Adenocarcinoma

Prognostic Significance of microRNA-7 and its Roles in the Regulation of Cisplatin Resistance in Lung Adenocarcinoma

miR‐17‐5p promotes proliferation and epithelial‐mesenchymal transition in human osteosarcoma cells by targeting SRC kinase signaling inhibitor 1

Reduction of miR-132-3p contributes to gastric cancer proliferation by targeting MUC13

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