MicroRNA-29a May Influence Myopia Development by Regulating Collagen I

Zhu, Yi; Zhang, Yingjie; Jiang, Run De; Zhao, Keke; Zhou, Jibo

doi:10.1080/02713683.2021.1998542

Cited by 8 publications

(7 citation statements)

References 35 publications

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“…Tears, vitreous humor, and AqH are main sources of fluids containing extracellular miRNAs in eyes [ 32 , 33 ]. Several reports have revealed highly expressed miR-29a in AqH of myopia patients and it prevented collagen I synthesis in SF cells, indicating its importance in myopia development [ 34 ]. The AqH analysis is very useful to study the molecular mechanism of axial elongation essential for myopia and to understand the role in HM, which will help to develop new therapeutic approaches [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

The Connection between High Myopia Patients and MiR-708a or MiR-148 Expression Levels in Aqueous Studies of Visual Acuity

Shen¹,

Hong²,

Yu³

et al. 2022

BioMed Research International

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Myopia goes far beyond the inconvenience it brings. It is a prevailing and vision-threatening eye disease, especially in Asia. Aberrantly expressed miR-708a and miR-148 are critical for accurate diagnosis, good prognosis, and precise response prediction of myopia. In this paper, we aim to examine the potential contributions of miR-708a, miR-148a, and PAX6 to high myopia (HM). First, aqueous samples were taken from 25 exclusively HM eyes and 25 exclusively cataract eyes. For next-generation sequencing and bioinformatics analysis, RNA from sample 30one was used. Twenty more samples were used for RT-qPCR. 341 miRNAs in total were found in HM eyes; 249 mature miRNAs and 17 new miRNAs showed differential expression. The expression of hsa-miR-127-3p, hsa-let-7i-5p, and hsa-miR-98-5p was identified using RT-qPCR. MiR-708a and miR-148, which may be linked to the development of myopia and serve as possible biomarkers, are notably highly expressed in atrial tissues of HM patients. Our findings may help deepen the understanding of the mechanisms behind the high expression of miR-708a and miR-148 in atrial tissues of patients with HM.

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Section: Discussionmentioning

confidence: 99%

The Connection between High Myopia Patients and MiR-708a or MiR-148 Expression Levels in Aqueous Studies of Visual Acuity

Shen¹,

Hong²,

Yu³

et al. 2022

BioMed Research International

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“…Still, several shortcomings exist in our study. In current proteomic studies involving sampling aqueous humor from patients for expression profiling, there are some sampling biases. ,, Due to ethical considerations, sampling intraocular fluid from normal individuals is not permissible. When using cataract patients as a control group, the protein expression patterns in their eyes may not be entirely identical to those of individuals with high myopia only.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Aqueous Humor Reveals Biochemical Disparities in the Eyes of High Myopic Patients

Du,

Liu,

Zhou

et al. 2024

J. Proteome Res.

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Myopia accounts for a significant proportion of visual lesions worldwide and has the potential to progress toward pathological myopia. This study aims to reveal the difference in protein content in aqueous humor between high myopic and nonhigh myopic patients, as well as better understand the dysregulation of proteins in myopic eyes. Aqueous humor was collected for liquid chromatograph mass spectrometer (LC/MS) analysis from 30 individual eyes that underwent phacoemulsification and intraocular lens (IOL) implantation. Results showed that a total of 190 differentially expressed proteins were identified, which revealed their involvement in cell metabolism, immune and inflammatory response, and system and anatomical structure. Further analysis focused on 15 intensively interacted hub proteins, encompassing functions related to complement cascades, lipoprotein metabolism, and fibrin biological function. Subsequent validations demonstrated elevated levels of APOE (apolipoprotein E), C3 (complement 3), and AHSG (α-2-HS-glycoprotein) in the high myopia group (31 eyes of cataracts and 45 eyes of high myopia with cataracts). AHSG had a significant positive correlation with axial length in high myopic patients, with good efficacy in distinguishing between myopic and nonmyopic groups. AHSG may be a potential indicator of the pathological severity and participator in the pathological progress of high myopia. This study depicted differential expression characteristics of aqueous humor in patients with high myopia and provided optional information for further experimental research on exploring the molecular mechanisms and potential therapeutic targets for high myopia. Data are available via ProteomeXchange with the identifier PXD047584.

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“…Recent research has highlighted the importance of epigenetic modifications in the development of myopia 3 – 5 . microRNAs (miRNAs) exert control over gene expression through their interaction with the 3’ untranslated region (UTR) of target (messenger RNAs) mRNAs, causing a suppression of translation or mRNA degradation.…”

Section: Introductionmentioning

confidence: 99%

“…You et al identified that vitreous miR-145-5p and miR-143-3p are potentially involved in myopic maculopathy development and relate to insulin resistance 10 . Zhu et al 5 revealed that the MiR-29a expression was elevated in the aqueous humor of individuals diagnosed with myopia. Furthermore, in vitro experiments demonstrated that MiR-29a effectively suppressed the production of type I collagen in human scleral fibroblast cells.…”

Section: Introductionmentioning

confidence: 99%

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MiR-204-5p may regulate oxidative stress in myopia

Jiang,

Hong,

Guo

et al. 2024

Sci Rep

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The mechanisms underlying myopia remain not fully understood. We proposed to examine the function and underlying mechanisms of miR-204-5p in myopia development. The miR-204-5p expression level was assessed in the vitreous humor (VH) of a cohort consisting of 11 patients with high myopia (HM) and 16 control patients undergoing vitrectomy. Then the functional implications of miR-204-5p in ARPE-19 cells were assessed. Thioredoxin-interacting protein (TXNIP) was found as a possible target of miR-204-5p through mRNA sequencing, and its interaction with miR-204-5p was confirmed employing luciferase assay and western blotting. Furthermore, the miR-204-5p function in regulating oxidative stress was examined by measuring reactive oxygen species (ROS) accumulation. The results indicated a significant reduction of miR-204-5p in the VH of HM patients. Overexpression of miR-204-5p suppressed cell proliferation, migration, invasion, and apoptosis in ARPE-19 cells. The direct targeting of miR-204-5p on TXNIP has been confirmed, and its downregulation mediated the miR-204-5p impacts on ARPE-19 cells. Moreover, miR-204-5p overexpression significantly reduced ROS accumulation by targeting TXNIP. Our findings revealed the possible contribution of the miR-204-5p/TXNIP axis in myopia development by regulating oxidative stress, which may provide new targets to combat this prevalent and debilitating condition.

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MicroRNA-29a May Influence Myopia Development by Regulating Collagen I

Cited by 8 publications

References 35 publications

The Connection between High Myopia Patients and MiR-708a or MiR-148 Expression Levels in Aqueous Studies of Visual Acuity

The Connection between High Myopia Patients and MiR-708a or MiR-148 Expression Levels in Aqueous Studies of Visual Acuity

Comparative Proteomic Analysis of Aqueous Humor Reveals Biochemical Disparities in the Eyes of High Myopic Patients

MiR-204-5p may regulate oxidative stress in myopia

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