MicroRNA-281 regulates the expression of ecdysone receptor (EcR) isoform B in the silkworm, Bombyx mori

Jiang, Jiali; Ge, Xie; Li, Zhiqian; Wang, Yueqiang; Song, Qisheng; Stanley, David; Tan, Anjiang; Huang, Yongping

doi:10.1016/j.ibmb.2013.05.002

Cited by 77 publications

(62 citation statements)

References 39 publications

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“…miRNA target predictions Three miRNA prediction software packages, PITA, miRanda and microTar, 25,31,32 were used jointly to predict the miR-2 targets. Thresholds were set to a score of 140 for miRanda (default), ddG 0 for PITA, and energy 0.5 for microTar.…”

Section: Methodsmentioning

confidence: 99%

“…Thresholds were set to a score of 140 for miRanda (default), ddG 0 for PITA, and energy 0.5 for microTar. 25,31,32 The 3 0 -end UTRs sequences of B. mori genes were downloaded from NCBI (http://www.ncbi.nlm.nih.gov/).…”

Section: Methodsmentioning

confidence: 99%

“…Three miRNA target-prediction tools, miRanda, PITA and microTar, 25,31,32 were used in combination to identify 113 putative miR-2 family targets in B. mori (Table S1). Since the overexpression of the miR-2 cluster produced the abnormalities in wing disc development, we focused on 2 putative target genes, Bmawd and Bmfng (Table S2), which are well-known regulators in Notch signaling for insect wing development.…”

Section: Mir-2 Targets Bmawd and Bmfng To Regulate Wing Morphogenesismentioning

confidence: 99%

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MiR-2 family targetsawdandfngto regulate wing morphogenesis inBombyx mori

Lin

et al. 2015

RNA Biology

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(2015) MiR-2 family targets awd and fng to regulate wing morphogenesis in Bombyxmori, RNA Biology, 12:7, 742-748, DOI: 10.1080/15476286.2015 Keywords: CRISPR/Cas9, Gal4/UAS, miR-2 cluster, transgene, wing disc MicroRNAs (miRNAs) are post-transcriptional regulators that target specific mRNAs for repression and thus play key roles in many biological processes, including insect wing morphogenesis. miR-2 is an invertebrate-specific miRNA family that has been predicted in the fruit fly, Drosophila melanogaster, to be involved in regulating the Notch signaling pathway. We show here that miR-2 plays a critical role in wing morphogenesis in the silkworm, Bombyx mori, a lepidopteran model insect. Transgenic over-expression of a miR-2 cluster using a Gal4/UAS system results in deformed adult wings, supporting the conclusion that miR-2 regulates functions essential for normal wing morphogenesis. Two genes, abnormal wing disc (awd) and fringe (fng), which are positive regulators in Notch signaling, are identified as miR-2 targets and validated by a dual-luciferase reporter assay. The relative abundance of both awd and fng expression products was reduced significantly in transgenic animals, implicating them in the abnormal wing phenotype. Furthermore, somatic mutagenesis analysis of awd and fng using the CRISPR/Cas9 system and knock-out mutants also resulted in deformed wings similar to those observed in the miR-2 overexpression transgenic animals. The critical role of miR-2 in Bombyx wing morphogenesis may provide a potential target in future lepidopteran pest control.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Mir-2 Targets Bmawd and Bmfng To Regulate Wing Morphogenesismentioning

confidence: 99%

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MiR-2 family targetsawdandfngto regulate wing morphogenesis inBombyx mori

Lin

et al. 2015

RNA Biology

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“…Thus, it is plausible that miR‐276 enhances brm expression to promote developmental synchrony and provide insight into the regulation of developmental homeostasis in T. tabaci . The second highest expression is for miR‐281 with an expression value of 18,063 and might be involved in the development and metamorphosis of T. tabaci as recent studies showed that miR‐281 regulates the expression of ecdysone receptor ( EcR ) isoform B, in Bombyx mori (Jiang et al., 2013). Another interesting microRNA obtained in the current study was miR‐8, and it can target the Wingless signalling pathway to regulate secretion of yolk protein precursors by the female mosquito fat body and accumulation into the developing ovaries (Lucas et al., 2015, http://www.smartscitech.com/index.php/RD/article/view/815).…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide identification, expression profiling, and target gene analysis of microRNAs in the Onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae), vectors of tospoviruses (Bunyaviridae)

Rebijith

Asokan

Hande

et al. 2018

Ecology and Evolution

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Thrips tabaci Lindeman is an important polyphagous insect pest species estimated to cause losses of more than U.S. $1 billion worldwide annually. Chemical insecticides are of limited use in the management of T. tabaci due to the thigmokinetic behavior and development of resistance to insecticides. There is an urgent need to find alternative management strategies. Small noncoding RNAs (sncRNAs) especially microRNAs (miRNAs) hold great promise as key regulators of gene expression in a wide range of organisms. MiRNAs are a group of endogenously originated sncRNA known to regulate gene expression in animals, plants, and protozoans. In this study, we explored these RNAs in T. tabaci using deep sequencing to provide a basis for future studies of their biological and physiological roles in governing gene expression. Apart from snoRNAs and piRNAs, our study identified nine novel and 130 known miRNAs from T. tabaci. Functional classification of the targets for these miRNAs predicted that majority are involved in regulating transcription, translation, signal transduction and genetic information processing. The higher expression of few miRNAs (such as tta‐miR‐281, tta‐miR‐184, tta‐miR‐3533, tta‐miR‐N1, tta‐miR‐N7, and tta‐miR‐N9) in T. tabaci pupal and adult stages reflected their possible role in larval and adult development, metamorphosis, parthenogenesis, and reproduction. This is the first exploration of the miRNAome in T. tabaci, which not only provides insights into their possible role in insect metamorphosis, growth, and development but also offer an important resource for future pest management strategies.

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“…For insulin injection, 2 mg was injected into each larva (500 ng/mL, for a total of 4 mL), and the control group was injected with an equal volume of PBS. For the 20E injection, 500 ng 20E was used for each larva (100 ng/mL, for a total of 5 mL), 39,40 with DMSO as the solvent control.…”

Section: Construction Of Mutant Overexpression Plasmidsmentioning

confidence: 99%

Cyclin-dependent kinase regulatory subunit 1 promotes cell proliferation by insulin regulation

Liu¹,

Zhao²,

Wang

et al. 2015

Cell Cycle

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These authors contributed equally to this work.Keywords: body size, cell proliferation, CKS1, insulin, midgut Cyclin-dependent kinase regulatory subunit 1 (CKS1) helps regulate the cell cycle to increase cell number. However, the hormonal regulation on CKS1 expression is not well understood. We report that CKS1 is involved in the promotion of cell proliferation with insulin regulation in the lepidopteran insect Helicoverpa armigera. CKS1 is expressed in various tissues during the larval feeding stage. CKS1 knockdown results in larval death, body weight decrease, pupation time delay, and small-sized pupa formation. The underlying mechanism involves the blocking of cell proliferation and the repression of gene expression in the insulin pathway after CKS1 knockdown. CKS1 overexpression in the epidermal cell line results in cell proliferation. The N45 amino acid asparagine in the CKS domain is essential for the function of CKS in cell proliferation. CKS1 is upregulated by insulin via an insulin receptor, but is repressed by a high level of steroid hormone 20-hydroxyecdysone (20E). Results suggest that CKS1 promotes cell proliferation and body growth in coordination with the regulatory actions of insulin and steroid hormone 20E.

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MicroRNA-281 regulates the expression of ecdysone receptor (EcR) isoform B in the silkworm, Bombyx mori

Cited by 77 publications

References 39 publications

MiR-2 family targetsawdandfngto regulate wing morphogenesis inBombyx mori

MiR-2 family targetsawdandfngto regulate wing morphogenesis inBombyx mori

Genome‐wide identification, expression profiling, and target gene analysis of microRNAs in the Onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae), vectors of tospoviruses (Bunyaviridae)

Cyclin-dependent kinase regulatory subunit 1 promotes cell proliferation by insulin regulation

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