MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation

Zhang, Genxi; He, Ming‐Liang; Wu, Pengfei; Zhang, Xinchao; Zhou, Kaizhi; Li, Tingting; Zhang, Tao; Xie, Kaizhou; Dai, Guojun; Wang, Jinyu

doi:10.3390/cells10020423

Cited by 23 publications

(15 citation statements)

References 40 publications

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“…For each circRNA, the target miRNAs with the highest score were selected from 95 target miRNAs and then used to construct a circRNA-miRNA network (Figure 6). Some miRNAs that have been previously reported to be associated with the growth and development of skeletal muscle and the proliferation and differentiation of preadipocytes were found, including miR-140-3p, miR-129-5p, miR-424-5p, miR-27b-3p and miR-30a-3p [6, [37][38][39][40].…”

Section: Functional Analysis Of Circrnas As Mirna Spongesmentioning

confidence: 99%

Identification and characterization of circular RNAs in Longissimus dorsi muscle tissue from two goat breeds using RNA-Seq

Zhen

Zhang

et al. 2022

Mol Genet Genomics

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Background: Circular RNAs (circRNAs) are a class of non-coding RNA that play crucial roles in the development of skeletal muscle. However, little is known about the role of circRNAs in caprine skeletal muscle. In this study, the muscle ber size and expression pro les of circRNAs were compared in Longissimus dorsi muscle of Liaoning cashmere (LC) goats and Ziwuling black (ZB) goats with signi cant phenotypic differences in meat production performance, using hematoxylin and eosin staining and RNA-Seq, respectively.Results: The muscle ber size in LC goats were larger than those in ZB goats (P < 0.05). A total of 10,875 circRNAs were identi ed and 214 of these were differentially expressed between the two caprine breeds.The authentication and expression levels of 20 circRNAs were con rmed using reverse transcriptasepolymerase chain reaction (RT-PCR) and DNA sequencing. The parent genes of differentially expressed circRNAs were mainly enriched in connective tissue development, Rap1, cGMP-PKG, cAMP and Ras signaling pathway. Some miRNAs reportedly associated with skeletal muscle development and intramuscular fat deposition would be targeted by several differentially expressed circRNAs and the most highly expressed circRNA (circ_001086).Conclusion: These results provide an improved understanding of the functions of circRNAs in skeletal muscle development of goats.

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Section: Functional Analysis Of Circrnas As Mirna Spongesmentioning

confidence: 99%

Identification and characterization of circular RNAs in Longissimus dorsi muscle tissue from two goat breeds using RNA-Seq

Zhen

Zhang

et al. 2022

Mol Genet Genomics

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“…MiR-21-5p targets KLF3 and regulates skeletal muscle satellite cell proliferation and differentiation [ 32 ]. MicroR-27b-3p regulates the proliferation and differentiation of chicken primary myoblasts by targeting MSTN [ 33 ]. Moreover, miRNA-214 regulates chicken myoblast proliferation and differentiation by targeting TRMT61A [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

MiRNA sequencing of Embryonic Myogenesis in Chengkou Mountain Chicken

Shi

Liu

et al. 2022

BMC Genomics

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Background Skeletal muscle tissue is among the largest organ systems in mammals, essential for survival and movement. Embryonic muscle development determines the quantity and quality of muscles after the birth of an individual. MicroRNAs (miRNAs) are a significant class of non-coding RNAs that bind to the 3’UTR region of mRNA to regulate gene function. Total RNA was extracted from the leg muscles of chicken embryos in different developmental stages of Chengkou Mountain Chicken and used to generate 171,407,341 clean small RNA reads. Target prediction, GO, and KEGG enrichment analyses determined the significantly enriched genes and pathways. Differential analysis determined the significantly different miRNAs between chicken embryo leg muscles at different developmental stages. Meanwhile, the weighted correlation network analysis (WGCNA) identified key modules in different developmental stages, and the hub miRNAs were screened following the KME value. Results The clean reads contained 2047 miRNAs, including 721 existing miRNAs, 1059 known miRNAs, and 267 novel miRNAs. Many genes and pathways related to muscle development were identified, including ERBB4, MEF2C, FZD4, the Wnt, Notch, and MAPK signaling pathways. The WGCNA established the greenyellow module and gga-miR-130b-5p for E12, magenta module and gga-miR-1643-5p for E16, purple module and gga-miR-12218-5p for E19, cyan module and gga-miR-132b-5p for E21. Conclusion These results lay a foundation for further research on the molecular regulatory mechanism of embryonic muscle development in Chengkou mountain chicken and provide a reference for other poultry and livestock muscle development studies.

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“…Therefore, whether our research object miR-27b-3p can affect muscle atrophy through miR-27b-3p/PPARγ/ATGL pathway remains to be further studied. In chickens, miR-27b-3p significantly promoted the proliferation of chicken primary myoblasts (CPMs) by targeting myostatin (MSTN) and inhibited the differentiation of CPMs [ 39 ]. Interestingly, some studies have shown that in mice, excessive retinoic acid upregulates miR-27b-3p and targets α-dystrobrevin to inhibit myoblast proliferation and differentiation [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles

Yang

Wang

et al. 2022

Biomolecules

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As it is well known, muscle atrophy is a process in which protein degradation increases and protein synthesis decreases. This process is regulated by a variety of links. Among them, microRNAs play an essential role in this process, which has attracted widespread attention. In this paper, we find that miR-27b-3p and Cbl-b genes are significantly differentially expressed in the induced atrophy model. The dual-luciferase experiment and Western blot analysis confirmed that miR-27b-3p could regulate the expression of Cbl-b. In C2C12-differentiated myotubes, the overexpression of the Cbl-b gene showed that Cbl-b could upregulate the expression of MuRF-1 and Atrogin-1, which are related marker genes of muscle atrophy, at both the mRNA and protein levels, indicating that the Cbl-b gene can specifically affect muscle atrophy. The knockdown of the Cbl-b gene after C2C12-differentiated myotubes induced atrophy treatment can downregulate the expression of muscle-atrophy-related genes, indicating that manual intervention to downregulate the expression of Cbl-b has a certain alleviating effect on muscle atrophy. These data suggest that miR-27b-3p can regulate the expression of the Cbl-b gene and then exert a particular influence on muscle atrophy through the Cbl-b gene.

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MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation

Cited by 23 publications

References 40 publications

Identification and characterization of circular RNAs in Longissimus dorsi muscle tissue from two goat breeds using RNA-Seq

Identification and characterization of circular RNAs in Longissimus dorsi muscle tissue from two goat breeds using RNA-Seq

MiRNA sequencing of Embryonic Myogenesis in Chengkou Mountain Chicken

miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles

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