MicroRNA-27a promotes porcine myoblast proliferation by downregulating myostatin expression

Abstract

MicroRNAs are endogenous~22nt RNAs that negatively regulate gene expression at the posttranscriptional level via binding to the 3′-untranslated region (3′UTR) of target mRNAs. The microRNA miR-27a was reported to depress the expression of myostatin, a critical inhibitor of skeletal myogenesis, by binding to its 3′UTR in mouse. In this study, we cloned the full-length 3′UTR of porcine myostatin by rapid amplification of 3′-cDNA ends (3′-RACE) and demonstrated that the 3′UTR of porcine myostatin is targeted by miR-27a. The phenomenon that the level of myostatin inversely correlated with miR-27a was observed in fat and heart of pigs and also in proliferating porcine myoblasts. Besides, overexpression of miR-27a in porcine myoblasts promoted cell proliferation by reducing the expression of myostatin. Our data suggest that miR-27a positive regulates porcine myoblast proliferation via targeting myostatin.

Akirin2 regulates proliferation and differentiation of porcine skeletal muscle satellite cells via ERK1/2 and NFATc1 signaling pathways

“…Porcine skeletal muscle satellite cells were isolated from 3-day-old male Duroc × Yorkshire × Landrace (DLY) pigs as described previously39 with some modifications. Briefly, skeletal muscles were digested with 0.2% collagenase type II (Sigma) and then filtered successively through 200-mesh and 400-mesh cell sieves.…”

Myostatin: Expanding horizons

“…Further analysis revealed that miR‐27a/b‐mediated inhibition of myostatin has a role in promoting satellite cell (SC) activation, increasing myoblast proliferation, and preventing muscle wasting . Similarly, Yang et al, () have also demonstrated that miR‐27a positively regulates porcine myoblast proliferation through inhibiting myostatin .…”

Integrated Analyses Reveal Overexpressed Notch1 Promoting Porcine Satellite Cells’ Proliferation through Regulating the Cell Cycle

“…In skeletal muscle, highly expressed miRNAs are called myomiRs, which include miR-1, miR-133a, miR-133b, miR-206, miR-208a, miR-208b, miR-486 and miR-499 [ 23 ]. Besides the myomiRs, there are also many miRNAs regulating satellite cell myogenesis, for example miR-31 [ 24 ]; miR-27a [ 25 , 26 ], miR128 [ 27 ], miR-221/miR-222 [ 28 ], miR-682 [ 29 ] and miR-181 [ 30 ]; miR-186 [ 31 ], miR-24 [ 32 ], miR-214 [ 33 , 34 ], miR-26a [ 35 ], miR-29b/c [ 36 ], miR-322/424 [ 37 ] and miR-503 [ 37 ] have been identified to regulate the quiescence, proliferation and differentiation stage of satellite cells respectively. Both Notch signaling and miRNA have been identified to play critical roles in regulating myogenesis, and four miRNAs, miR-1, miR-206, miR-146a and 146b, have been reported to regulate skeletal muscle myogenesis via Notch signaling [ 38 , 39 , 40 , 41 ].…”