MicroRNA-27a inhibitors alone or in combination with perifosine suppress the growth of gastric cancer cells

Liu, Dongxiao; Sun, Qingmin; Song, Lina; Xu, Linlin; Luo, Xuan; Zhao, Xiaomei; Wang, Xuerong; Yang, Li

doi:10.3892/mmr.2012.1191

Cited by 9 publications

(2 citation statements)

References 32 publications

(31 reference statements)

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“…Liu et al have elucidated that miR-27a was highly expressed in human MGC-803 GC cells [14], which is in accordance with our study. Besides, it was also supported by a report that a high expression of miR-27a was found in GC tissues in contrast with their matched non-tumor adjacent tissues [36]. Taken together, we have further demonstrated that miR-27a may act as an oncogene to be associated with carcinogenesis in human GC.…”

Section: Discussionsupporting

confidence: 88%

Relationship between microRNA-27a and efficacy of neoadjuvant chemotherapy in gastric cancer and its mechanism in gastric cancer cell growth and metastasis

Cheng

et al. 2019

Bioscience Reports

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Objective: The aim of the present study is to investigate the relationship between microRNA-27a (miR-27a) and the efficacy of neoadjuvant chemotherapy in gastric cancer (GC) and its mechanism in the growth and metastasis of GC cells. Methods: The expression of miR-27a in serum of 74 GC patients received neoadjuvant chemotherapy was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Clinical value and prognosis of miR-27a expression in predicting the efficacy of neoadjuvant chemotherapy in GC were evaluated. Besides, GC cells with low miR-27a expression were transfected with miR-27a mimics, and cells with high miR-27a expression were transfected with miR-27a inhibitors and secreted frizzled-related protein 1 (SFRP1) siRNA. A series of experiments were applied for the determination of cell viability, invasion and migration of GC cells. Results: After neoadjuvant chemotherapy, the expression of miR-27a in serum of GC patients decreased significantly. Additionally, the expression of miR-27a in GC cell line was significantly higher than that in normal gastric mucosa cell line. Meanwhile, after down-regulating the expression of miR-27a in GC cells, the mRNA and protein expression of SFRP1 increased, the proliferation rate of cells slowed down, and the ability of invasion and migration decreased. Furthermore, combined with low expression of miR-27a and SFRP1, the proliferation rate of GC cells increased and the ability of invasion and migration increased. Conclusion: Collectively, our study highlights that the high expression of miR-27a indicates the poor efficacy and prognosis of neoadjuvant chemotherapy in GC patients. Down-regulation of miR-27a can inhibit the growth and metastasis of GC cells via up-regulation of SFRP1.

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Section: Discussionsupporting

confidence: 88%

Relationship between microRNA-27a and efficacy of neoadjuvant chemotherapy in gastric cancer and its mechanism in gastric cancer cell growth and metastasis

Cheng

et al. 2019

Bioscience Reports

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“…Our result has demonstrated that miR27a level of Colon cancer group was much higher than that of Normal and Colitis groups (P<0.05). A recent study suggested that miR27a may have a high expression level in gastric cancer (Liu et al, 2013). This is similar with the result made in our paper.…”

Section: Discussionsupporting

confidence: 83%

Effect of miR27a on Proliferation and Invasion in Colonic Cancer Cells

Gao¹,

Li²,

Liu³

2013

Asian Pacific Journal of Cancer Prevention

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The aim of this study was to detect the expression of miR196a, miR146a, miR27a and miR200a in patients with colon cancer, and investigate the effect of miR27a expression on proliferation and invasion in colonic cancer cells. RT-PCR was employed to detect the expression levels in colon cancers. Then, colon cancer cells were cultured and transfected with 100 nM of miR27a mimics (80 nmol/L) or 80 nM miR27a inhibitors (80 nmol/L) in 24-well plates. Proliferation and invasion of colonic cancer cells were then determined by CCK-8 and Transwell assays, respectively. Our data showed miR27a to be high-expressed in patients with colon cancer. In addition, proliferation and invasion in the miR27a mimic group were significantly higher than in the control group and negative group (P<0.05), while, proliferation and invasion in the miR27a inhibitor group were obviously lowered (P<0.05). In conclusion, high expression of miR27a may play an important role in enhancing proliferation and invasion of colon cancer cells. Keywords: Colon cancer cells -miR27a -proliferation -invasion RESEARCH ARTICLE Effect of miR27a on Proliferation and Invasion in Colonic Cancer CellsYang Gao*, Bao-Dong Li, Yong-Gang Liu investigate the role of miR27a expression on colon cancer cells proliferation and invasion.

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Cancer Genetics at a Glance: The Comprehensive Insights

Azarnezhad

Mehdipour

2017

Cancer Genetics and Psychotherapy

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MicroRNA-27a inhibitors alone or in combination with perifosine suppress the growth of gastric cancer cells

Cited by 9 publications

References 32 publications

Relationship between microRNA-27a and efficacy of neoadjuvant chemotherapy in gastric cancer and its mechanism in gastric cancer cell growth and metastasis

Relationship between microRNA-27a and efficacy of neoadjuvant chemotherapy in gastric cancer and its mechanism in gastric cancer cell growth and metastasis

Effect of miR27a on Proliferation and Invasion in Colonic Cancer Cells

Cancer Genetics at a Glance: The Comprehensive Insights

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