MicroRNA-27a-3p affects estradiol and androgen imbalance by targeting Creb1 in the granulosa cells in mouse polycytic ovary syndrome model

Wang, Mingming; Liu, Mei; Sun, Jing; Jia, Lina; Ma, Shuoqian; Gao, Jun; Xu, Yan; Zhang, Hongwen; Tsang, Suk Ying; Li, Xiangdong

doi:10.1016/j.repbio.2017.09.005

Cited by 48 publications

(33 citation statements)

References 24 publications

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“…GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mGCs, mouse granulosa cells; miR-205, microRNA-205; mRNA, messenger RNA; qRT-PCR, quantitative real-time polymerase chain reaction potential downstream target gene of miR-205 on the basis of CREB1 function in ovarian GCs. 23,24 A highly conserved putative miR-205 recognition sequence was found to bind to the 3′-UTR of CREB1 at positions 2036-2043, suggesting that this gene is a target of miR-205 ( Figure 4A). To further confirm the targeting of CREB1 by miR-205, the luciferase activity assay was performed for HEK293 cells.…”

Section: Creb1 Was a Direct Target Of Mir-205 In Mgcsmentioning

confidence: 99%

RETRACTED: MicroRNA‐205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1

Zhang

Wang

Lang

et al. 2018

J of Cellular Biochemistry

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MicroRNA‐205 (miR‐205) is involved in various physiological and pathological processes, but its biological function in follicular atresia remains unclear. In this study, we investigated miR‐205 expression in mouse granulosa cells (mGCs) and analyzed its functions in primary mGCs by performing a series of in vitro experiments. Quantitative real‐time polymerase chain reaction showed that miR‐205 expression was significantly higher in early atretic follicles and progressively atretic follicles than in healthy follicles. miR‐205 overexpression in mGCs significantly promoted apoptosis and caspase‐3/9 activities, as well as inhibited estrogen (E2) release and cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1, a key gene in E2 production) expression. Bioinformatics and luciferase reporter assays revealed that the gene encoding cyclic AMP response element (CRE)‐binding protein 1 (CREB1) was a direct target of miR‐205 in mGCs. CREB1 upregulation partially rescued the effects of miR‐205 on apoptosis, caspase‐3/9 activities, E2 production, and CYP19A1 expression on mGCs. These results indicate that miR‐205 might play an important role in ovarian follicular development and provide new insights into follicular atresia

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Section: Creb1 Was a Direct Target Of Mir-205 In Mgcsmentioning

confidence: 99%

RETRACTED: MicroRNA‐205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1

Zhang

Wang

Lang

et al. 2018

J of Cellular Biochemistry

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“…Therefore, we hypothesize that BDNF levels can be restored by antagomirs (anti‐miRNA) of miR‐206 and miR‐381, as previously found in a model of Alzheimer's disease (Lee et al, ). Previous studies (Sun & Trajkovski, ; Wang et al, ) reported that up‐regulation of miR‐27a, which interacts with mRNA of PPAR‐α and CREB1, decreased protein levels of PPAR‐α. In our study, we found the same modulation in diabetic retina for PPAR‐α but not for CREB1 protein levels.…”

Section: Discussionmentioning

confidence: 94%

Retinal and circulating miRNA expression patterns in diabetic retinopathy: An in silico and in vivo approach

Platania

Maisto

Trotta

et al. 2019

British J Pharmacology

102

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Background and Purpose Diabetic retinopathy, a secondary complication of diabetes mellitus, can lead to irreversible vision loss. Currently, no treatment is approved for early phases of diabetic retinopathy. Modifications of the expression pattern of miRNAs could be involved in the early retinal damage of diabetic subjects. Therefore, we aimed at identification of dysregulated miRNAs–mRNA interactions that might be biomarkers and pharmacological targets for diagnosis and treatment of early diabetic retinopathy. Methods A focused set of miRNAs was predicted through a bioinformatic analysis accessing to Gene Expression Omnibus dataset and enrichment of information approach (GENEMANIA‐Cytoscape). Identification of miRNAs–mRNA interactions was carried out with miRNET analysis. Diabetes was induced in C57BL6J mice by streptozotocin and samples analysed at 5 and 10 weeks after diabetes induction. Retinal ultrastructure of diabetic mice was analysed through electron microscopy. We used Real‐time PCR, western blot analysis, elisa, and immunohistochemistry to study expression of miRNAs and possible targets of dysregulated miRNAs. Key Results We found that miR‐20a‐5p, miR‐20a‐3p, miR‐20b, miR‐106a‐5p, miR‐27a‐5p, miR‐27b‐3p, miR‐206‐3p, and miR‐381‐3p were dysregulated in the retina and serum of diabetic mice. VEGF, brain‐derived neurotrophic factor (BDNF), PPAR‐α, and cAMP response element‐binding protein 1 (CREB1) are targets of dysregulated miRNAs, which then modulated protein expression in diabetic retina. We found structural modifications in retinas from diabetic mice. Conclusions and Implications Serum and retina of diabetic mice express eight dysregulated miRNAs, which modified the expression of VEGF, BDNF, PPAR‐α, and CREB1, before vasculopathy in diabetic retinas.

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“…Understanding the expression patterns of miRNAs during the different phases of reproductive cycle (luteal or follicular phase) of mammals such as cattle would provide a better insight about post-transcriptional gene regulation underlining follicular atresia and oocyte ovulation [31,32] or to identify miRNAs governing the production of steroid essential for the normal processes of folliculogenesis [35][36][37][38][39]. Nevertheless, aberrant expression of developmentally relevant miRNAs during critical period of the reproductive cycle could negatively affect follicular development resulting for anovulation or ovulation of infertile oocytes.…”

Section: The Role Of Cellular Mirnas In Follicular Development and Oomentioning

confidence: 99%

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

Salilew‐Wondim

Gebremedhn

Hoelker

et al. 2020

IJMS

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The genetic codes inscribed during two key developmental processes, namely gametogenesis and embryogenesis, are believed to determine subsequent development and survival of adult life. Once the embryo is formed, its further development mainly depends on its intrinsic characteristics, maternal environment (the endometrial receptivity), and the embryo–maternal interactions established during each phase of development. These developmental processes are under strict genetic regulation that could be manifested temporally and spatially depending on the physiological and developmental status of the cell. MicroRNAs (miRNAs), one of the small non-coding classes of RNAs, approximately 19–22 nucleotides in length, are one of the candidates for post-transcriptional developmental regulators. These tiny non-coding RNAs are expressed in ovarian tissue, granulosa cells, testis, oocytes, follicular fluid, and embryos and are implicated in diverse biological processes such as cell-to-cell communication. Moreover, accumulated evidences have also highlighted that miRNAs can be released into the extracellular environment through different mechanisms facilitating intercellular communication. Therefore, understanding miRNAs mediated regulatory mechanisms during gametogenesis and embryogenesis provides further insights about the molecular mechanisms underlying oocyte/sperm formation, early embryo development, and implantation. Thus, this review highlights the role of miRNAs in mammalian gametogenesis and embryogenesis and summarizes recent findings about miRNA-mediated post-transcriptional regulatory mechanisms occurring during early mammalian development.

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MicroRNA-27a-3p affects estradiol and androgen imbalance by targeting Creb1 in the granulosa cells in mouse polycytic ovary syndrome model

Cited by 48 publications

References 24 publications

RETRACTED: MicroRNA‐205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1

RETRACTED: MicroRNA‐205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1

Retinal and circulating miRNA expression patterns in diabetic retinopathy: An in silico and in vivo approach

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

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