MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting the<i>INSIG1</i>gene

Wang, Hui; Luo, Jun; Zhang, Tianying; Tian, Hui; Ma, Yue; Xu, Huifen; Yao, Dawei; Loor, Juan J.

doi:10.1080/15476286.2016.1164365

Cited by 50 publications

(46 citation statements)

References 58 publications

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“…Additionally, functional validation has directly linked a number of miRNAs with regulatory roles in milk synthesis, fatty acid metabolism and cellular differentiation. Down-regulation of miR-26a/b and their host genes ( CTDSPL, CTDSP2 and CTDSP1 ) in goat mammary epithelial cells was shown to decrease the expression of genes related to fatty acid synthesis ( PPARG, LXRA, SREBF1, FASN, ACACA, GPAM, LPIN1, DGAT1 and SCD1 ), triacylglycerol accumulation and unsaturated fatty acid synthesis as well as demonstration of a functional association between miR-26a/b, their host genes and INSIG1 16. MiR-155a was observed to regulate the expression of growth hormone receptor and decrease bovine mammary epithelial cell viability and lactation12.…”

mentioning

confidence: 93%

MicroRNA roles in signalling during lactation: an insight from differential expression, time course and pathway analyses of deep sequence data

Ngoc

Dudemaine

et al. 2017

Sci Rep

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The study examined microRNA (miRNA) expression and regulatory patterns during an entire bovine lactation cycle. Total RNA from milk fat samples collected at the lactogenesis (LAC, day1 [D1] and D7), galactopoiesis (GAL, D30, D70, D130, D170 and D230) and involution (INV, D290 and when milk production dropped to 5 kg/day) stages from 9 cows was used for miRNA sequencing. A total of 475 known and 238 novel miRNAs were identified. Fifteen abundantly expressed miRNAs across lactation stages play regulatory roles in basic metabolic, cellular and immunological functions. About 344, 366 and 209 miRNAs were significantly differentially expressed (DE) between GAL and LAC, INV and GAL, and INV and LAC stages, respectively. MiR-29b/miR-363 and miR-874/miR-6254 are important mediators for transition signals from LAC to GAL and from GAL to INV, respectively. Moreover, 58 miRNAs were dynamically DE in all lactation stages and 19 miRNAs were significantly time-dependently DE throughout lactation. Relevant signalling pathways for transition between lactation stages are involved in apoptosis (PTEN and SAPK/JNK), intracellular signalling (protein kinase A, TGF-β and ERK5), cell cycle regulation (STAT3), cytokines, hormones and growth factors (prolactin, growth hormone and glucocorticoid receptor). Overall, our data suggest diverse, temporal and physiological signal-dependent regulatory and mediator functions for miRNAs during lactation.

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mentioning

confidence: 93%

MicroRNA roles in signalling during lactation: an insight from differential expression, time course and pathway analyses of deep sequence data

Ngoc

Dudemaine

et al. 2017

Sci Rep

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“…Total RNA was obtained from mammary gland tissue of each goat using the Trizol reagent (Invitrogen, USA) according to the manufacturer's protocol. The expression of the miR-26 family and their host genes was detected with primers reported previously (Wang et al, 2016), and analysed using the 2 -ΔΔCt method. The 5S rRNA was used as reference gene for miR-26 family expression experiment, and mRNA expression was normalized to ubiquitously expressed transcript (UXT), mitochondrial ribosomal protein L39(MRPL39) and ribosomal protein S9(RPS9), all the reference primers used were as reported previously (Wang et al, 2016).…”

Section: Twenty-four Mid-lactation (120 D After Parturition) Xinongmentioning

confidence: 99%

“…The function of a miRNA is ultimately defined by its effects on target genes expression. A previous study showed that the miR-26 family is involved in lipid synthesis in human, goat, and mice (Ning et al, 2017;Song et al, 2014;Wang et al, 2016), but the molecular mechanisms are unclear. To address the question of how the miR-26 family functions in concert with their target genes to provide some molecular insight into the lipid synthesis process, we used PicTar and TargetScan to predict miR-26 family targets.…”

Section: Twenty-four Mid-lactation (120 D After Parturition) Xinongmentioning

confidence: 99%

“…The miR-26 family is involved in adipocyte development and acquisition of brown adipocyte characteristics in humans (Karbiener et al, 2014). Down-regulation of the miR-26 family in goat mammary epithelial cells (GMEC) decreased the expression of genes related to fatty acid synthesis, triacylglycerol accumulation and unsaturated fatty acid synthesis, and miR-26 family display a similar trend of expression as their host genes (CTDSP family) over the lactation cycle (Wang et al, 2016). The genomic loci of miR-26a and miR-26b are localized in the introns of genes encoding the protein family C-terminal domain RNA polymerase polypeptide a small phosphatase (CTDSP) (Dill, Linder, Fehr, & Fischer, 2012;Ying et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Association between the expression of miR‐26 and goat milk fatty acids

Wang

Zhu

et al. 2018

Reprod Domestic Animals

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microRNA (miRNA) are small noncoding RNA that regulate protein abundance and are involved in diverse aspects of cellular function including aspects of lipid metabolism in mammary gland of ruminants. Although our previous studies showed that the miR-26 family and its host genes control components of the cellular fatty acid metabolic machinery in goat mammary epithelial cells, a direct relationship between the miR-26 family and milk fatty acids remains unknown. Bioinformatics analysis in this study indicated that the miR-26 family targets belong to the PI3K-Akt signalling pathway, MAPK signalling pathway, and fatty acid biosynthesis pathway. Studies on the relationship of miR-26 family and their host genes with milk composition during mid-lactation revealed that the expression of the miR-26 family and their host genes were associated with total fat yield and short-chain, medium-chain and long-chain fatty acid content, but not lactose or milk protein content. In addition, a significant positive correlation was detected for the expression of the miR-26 family with C16:1 and C18:3 in milk fat. Taken together, our findings demonstrate that the expression of miR-26 is directly related to milk fatty acid composition and underscores the significance of miRNAs in milk fat synthesis regulation.

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“…However, this study did not examine adipogenesis or changes in the hormones leptin, insulin, and TNF-alpha 105 . Nonetheless, a number of studies have shown the impact of miRNAs on fatty acid oxidation and lipogenesis 83,106 .…”

Section: Cardiovascular Risk Factorsmentioning

confidence: 99%

The role of miRNAs in cardiovascular disease risk factors

et al. 2016

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Coronary artery disease and atherosclerosis are complex pathologies that develop over time due to genetic and environmental factors. Differential expression of miRNAs has been identified in patients with coronary artery disease and atherosclerosis, however, their association with cardiovascular disease risk factors, including hyperlipidemia, hypertension, obesity, diabetes, lack of physical activity and smoking, remains unclear. This review examines the role of miRNAs as either biomarkers or potential contributors to the pathophysiology of these aforementioned risk factors. It is intended to provide an overview of the published literature which describes alterations in miRNA levels in both human and animal studies of cardiovascular risk factors and when known, the possible mechanism by which these miRNAs may exert either beneficial or deleterious effects. The intent of this review is engage clinical, translational, and basic scientists to design future collaborative studies to further elucidate the potential role of miRNAs in cardiovascular diseases.

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MicroRNA-26a/b and their host genes synergistically regulate triacylglycerol synthesis by targeting theINSIG1gene

Cited by 50 publications

References 58 publications

MicroRNA roles in signalling during lactation: an insight from differential expression, time course and pathway analyses of deep sequence data

MicroRNA roles in signalling during lactation: an insight from differential expression, time course and pathway analyses of deep sequence data

Association between the expression of miR‐26 and goat milk fatty acids

The role of miRNAs in cardiovascular disease risk factors

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