MicroRNA‑23a inhibits endometrial cancer cell development by targeting SIX1

Abstract

The present study focused on exploring the inhibitory mechanism of microRNA (miR)-23a in endometrial cancer. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to investigate miR-23a expression in endometrial tissues and endometrial cancer cells. A colony formation assay using crystal violet staining was performed to compare cell proliferation, while wound-healing and Transwell assays were performed to compare cell migration and invasion. Subsequently, bioinformatics and a luciferase reporter gene assay were used to investigate the effect of miR-23a on sine oculis homeobox homolog 1 (SIX1) expression, and the biological function of SIX1 was analyzed. Additionally, a nude mouse tumorigenicity assay was performed to test the inhibitory effect of miR-23a and Taxol ® therapy in endometrial cancer. Finally, immunohistochemistry and RT-qPCR were used to explore the association between miR-23a and SIX1 expression in endometrial cancer tissues. miR-23a was underexpressed in endometrial cancer tissues compared with in para-carcinoma tissues, and the overexpression of miR-23a inhibited proliferation and invasion of endometrial cancer cells. Furthermore, SIX1 was demonstrated to be a downstream target of miR-23a, and miR-23a reduced SIX1 expression. Additionally, SIX1 inversely promoted cell proliferation, migration and invasion. In addition, the effects of reduced cell proliferation and increased cell invasion following miR-23a overexpression could be reversed by adding SIX1 to in vitro culture. Furthermore, the inhibitory effect of miR-23a and Taxol therapy, which reduced SIX1 expression in endometrial cancer, was demonstrated in vivo. Finally, a negative association between miR-23a and SIX1 expression was demonstrated in endometrial cancer tissues. The results of the present study revealed that miR-23a may inhibit endometrial cancer development by targeting SIX1.

Seminal Plasma Modulates miRNA Expression by Sow Genital Tract Lining Explants

“…Similarly, miR-574-5p was dysregulated in the mediastinal lymph node in pigs infected with porcine circovirus type 2 and was predicted to be involved in the regulation of the T cell receptor signaling pathway [ 79 ]. Related to reproductive processes, a dysregulation in miR-23a and miR-574 family members has been related to pathological pregnancy [ 80 , 81 ] and endometrial cancer in humans [ 82 ]. Therefore, the literature indicates that these two miRNAs play a key role in immune and reproductive processes, suggesting that they could also be involved in the modulation of the female immune response.…”

Overexpression of miR-331 Indicates Poor Prognosis and Promotes Progression of Breast Cancer

“…For example, in NSCLC, miR-133a inhibits cell proliferation with poor prognosis by targeting YES1 [16]. miR-23a was downregulated in endometrial cancer, which inhibited the development of endometrial cancer by targeting SIX1 [17]. In contrast, in gastric cancer, miR-23a was upregulated with the promoting role in gastric cancer through targeting SPRY2 [18].…”

MicroRNA‑448 modulates the progression of neuropathic pain by targeting sirtuin 1

“…miRNAs exert their functions by binding to the target genes at a post-transcriptional level (33,34). Previous evidence has suggested that miR-448 exhibits differing functions in human diseases.…”