MicroRNA‐223 inhibits lipopolysaccharide‐induced inflammatory response by directly targeting <i>Irak1</i> in the nucleus pulposus cells of intervertebral disc

Wang, Hua; Pān, Hào; Zhang, Hu; Xu, Chunfang; Zhao, Junyan

doi:10.1002/iub.1747

Cited by 34 publications

(29 citation statements)

References 38 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiR - 223 was shown to be downregulated in the lungs of rats exposed to CS [80]. It has been shown that the overexpression of miR - 223 suppresses LPS-induced NF - κB signaling by targeting IL - 1 receptor-associated kinase 1 [184]. Recent data have revealed the upregulation of miR - 223 as a novel biomarker in subsets of patients with inflammatory bowel disease [185].…”

Section: Epigenetics and Inflammationmentioning

confidence: 99%

The role of cigarette smoke-induced epigenetic alterations in inflammation

Zong

Liu

et al. 2019

Epigenetics & Chromatin

125

View full text Add to dashboard Cite

Background: Exposure to cigarette smoke (CS) is a major threat to human health worldwide. It is well established that smoking increases the risk of respiratory diseases, cardiovascular diseases and different forms of cancer, including lung, liver, and colon. CS-triggered inflammation is considered to play a central role in various pathologies by a mechanism that stimulates the release of pro-inflammatory cytokines. During this process, epigenetic alterations are known to play important roles in the specificity and duration of gene transcription. Main text: Epigenetic alterations include three major modifications: DNA modifications via methylation; various posttranslational modifications of histones, namely, methylation, acetylation, phosphorylation, and ubiquitination; and non-coding RNA sequences. These modifications work in concert to regulate gene transcription in a heritable fashion. The enzymes that regulate these epigenetic modifications can be activated by smoking, which further mediates the expression of multiple inflammatory genes. In this review, we summarize the current knowledge on the epigenetic alterations triggered by CS and assess how such alterations may affect smoking-mediated inflammatory responses. Conclusion: The recognition of the molecular mechanisms of the epigenetic changes in abnormal inflammation is expected to contribute to the understanding of the pathophysiology of CS-related diseases such that novel epigenetic therapies may be identified in the near future.

show abstract

Section: Epigenetics and Inflammationmentioning

confidence: 99%

The role of cigarette smoke-induced epigenetic alterations in inflammation

Zong

Liu

et al. 2019

Epigenetics & Chromatin

125

View full text Add to dashboard Cite

show abstract

“…The previously reported close association between cul4B and inflammation suggested that there might an anti-inflammatory effect exerted by Syra in the survival of damaged nP cells. Indeed, the onset of inflammation induced by LPS in NP cells and the anti-inflammatory effect of SyrA has been confirmed in many prior reports (37)(38)(39)(40)(41). deGs in the 'apoptosis-multiple species' pathway include aPaF1, Birc3 and Bcl2.…”

Section: Lps-stimulated Groups Syra-reversed Groups -----------------mentioning

confidence: 61%

“…notably, the level of cullin 4B (cul4B) was found to be significantly upregulated in IDD samples compared with the controls, and this was positively correlated with the severity of idd (36). in addition, the expression of cul4B was enhanced after treatment with the inflammatory factors interleukin (il)-6 and tumour necrosis factor (TnF)-α, and reversed by il-6 and TnF-α inhibitors in primary nP cells (37). The previously reported close association between cul4B and inflammation suggested that there might an anti-inflammatory effect exerted by Syra in the survival of damaged nP cells.…”

Section: Lps-stimulated Groups Syra-reversed Groups -----------------mentioning

confidence: 97%

Gene expression profile of lipopolysaccharide‑induced apoptosis of nucleus pulposus cells reversed by syringic acid

et al. 2020

View full text Add to dashboard Cite

apoptosis of nucleus pulposus (nP) cells has an important role in the process of intervertebral disc degeneration (idd), and the search for novel compounds to prevent apoptosis from occurring is urgently required. in the present study, syringic acid (Syra) was found to exhibit no cytotoxicity on nP cells, and was able to reverse the cytotoxicity, as well as the abnormal expression of Bcl-2 and caspase-3, that were induced by lipopolysaccharide (lPS). The transcriptomes of each group were then analyzed using rna-Seq. a total of 65 differentially expressed genes (deGs) were identified in LPS-stimulated groups (lPS group vs. control group), 819 DEGs were identified in the SyrA-reversed groups (Syra plus lPS group vs. lPS group), and a further 25 deGs were identified in the Syra plus lPS group compared with the control group. reverse transcription-quantitative Pcr validation indicated that the alterations in expression of uroplakin 3B-like 1 (uPK3Bl1), voltage-dependent calcium channel subunit α-2/δ-1 (cacna2d1) and polo-like kinase 4 (PlK4) were consistent with the corresponding results of rna-Seq, and that these genes were involved in both lPS-stimulation and Syra-reversion processes. Kyoto encyclopedia of Genes and Genomes analyses indicated that the deGs in Syra-reversed groups were involved in, amongst other pathways, 'autophagy-other' and 'apoptosis-multiple species'. in conclusion, the addition of Syra to the nP cells co-incubated with lPS appeared to help prevent the abnormal expression of mrnas and apoptosis that had been identified in nP cells incubated with lPS alone. The potential mechanism underlying the reversion of Syra might be attributed to the regulation of cacna2d1 and PlK4.

show abstract

“…However, miR-233 can also target other genes, such as Irak1. 18 Our future studies will investigate the involvement of other targets of miR-233 in CSCC.…”

Section: Discussionmentioning

confidence: 99%

<p>LncRNA MAGI2-AS3 Affects Cell Invasion and Migration of Cervical Squamous Cell Carcinoma (CSCC) via Sponging miRNA-233/EPB41L3 Axis</p>

Hou¹,

Zhang²,

Fan³

et al. 2020

CMAR

View full text Add to dashboard Cite

Introduction: The incidence of cervical squamous cell carcinoma (CSCC) has expanded in recent years. However, the function of long non-coding RNA (lncRNA) MAGI2-AS3 in the occurrence and progression of CSCC remains unclear. Therefore, the role of lncRNA MAGI2-AS3 in cervical squamous cell carcinoma (CSCC) was investigated in our study. Methods: We used qRT-PCR analysis to identify the level of MAGI2-AS3 mRNA expression in CSCC clinical samples and cell lines. We investigated cell migration and invasion of CSCC cells transfected with MAGI2-AS3, miR-233 mimic, or EPB41L3 with transwell assays. Bioinformatics analysis and a luciferase reporter assay were employed to predict the interaction between MAGI2-AS3 and miR-233. Results: We found that MAGI2-AS3 and EPB41L3 were both downregulated in CSCC and the expression of this two was positively correlated. Bioinformatics analysis showed that MAGI2-AS3 might bind to miR-233, which could directly target EPB41L3. In CSCC cells, overexpression of MAGI2-AS3 led to upregulated, while overexpression of miRNA-233 led to downregulated expression of EPB41L3. However, MAGI2-AS3 and miR-233 did not affect the expression of each other. In addition, overexpression of MAGI2-AS3 and EPB41L3 led to inhibited cancer cell invasion and migration, while overexpression of miR-233 played an opposite role and attenuated the effects of overexpressing MAGI2-AS3. Conclusion: MAGI2-AS3 may sponge miR-233 to upregulate EPB41L3, thereby inhibiting CSCC cell invasion and migration.

show abstract

MicroRNA‐223 inhibits lipopolysaccharide‐induced inflammatory response by directly targeting Irak1 in the nucleus pulposus cells of intervertebral disc

Cited by 34 publications

References 38 publications

The role of cigarette smoke-induced epigenetic alterations in inflammation

The role of cigarette smoke-induced epigenetic alterations in inflammation

Gene expression profile of lipopolysaccharide‑induced apoptosis of nucleus pulposus cells reversed by syringic acid

<p>LncRNA MAGI2-AS3 Affects Cell Invasion and Migration of Cervical Squamous Cell Carcinoma (CSCC) via Sponging miRNA-233/EPB41L3 Axis</p>

Contact Info

Product

Resources

About