MicroRNA-223-3p inhibits vascular calcification and the osteogenic switch of vascular smooth muscle cells

Han, Yingchun; Zhang, Jichao; Huang, Shan; Cheng, Ni; Zhang, Congcong; Li, Yulin; Wang, Xiaonan; Liu, Jinghua; You, Bo; Du, Jie

doi:10.1016/j.jbc.2021.100483

Cited by 36 publications

(33 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the 195 DEmiRNAs identified, the most was functionally annotated, such as miR-7a-5p, miR-223-3p, miR-22-3p, miR-146a-5p, miR-378a-3p, miR-467a-5p, miR-532-5p, miR-652-3p, miR-871-3p and miR-96-5p ( Coffey et al, 2019 ; Ota et al, 2019 ; Chu et al, 2020 ; Du et al, 2020 ; Liang et al, 2020 ; Yan et al, 2020 ; Wang X. et al, 2021 ; Gajeton et al, 2021 ; Han et al, 2021 ; Kinoshita et al, 2021 ). Unlike the DEmiRNAs, most of the DElncRNAs were not annotated with unknown functions.…”

Section: Resultsmentioning

confidence: 99%

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

Liu

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The larvae of Echinococcus multilocularis causes alveolar echinococcosis, which poses a great threat to the public health. However, the molecular mechanisms underlying the host and parasite interactions are still unclear. Exploring the transcriptomic maps of mRNA, miRNA and lncRNA expressed in the liver in response to E. multilocularis infection will help us to understand its pathogenesis. Using liver perfusion, different cell populations including the hepatic cells, hepatic stellate cells and Kupffer cells were isolated from mice interperitoneally inoculated with protoscoleces. Their transcriptional profiles including lncRNAs, miRNAs and mRNAs were done by RNA-seq. Among these cell populations, the most differentially-expressed (DE) mRNA, lncRNAs and miRNAs were annotated and may involve in the pathological processes, mainly including metabolic disorders, immune responses and liver fibrosis. Following the integrative analysis of 38 differentially-expressed DEmiRNAs and 8 DElncRNAs, the lncRNA-mRNA-miRNA networks were constructed, including F63-miR-223-3p-Fbxw7/ZFP36/map1b, F63-miR-27-5p-Tdrd6/Dip2c/Wdfy4 and IFNgAS1-IFN-γ. These results unveil the presence of several potential lncRNA-mRNA-miRNA axes during E. multilocularis infection, and further exploring of these axes may contribute to better understanding of the pathogenic mechanisms.

show abstract

Section: Resultsmentioning

confidence: 99%

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

Liu

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…miR-223 regulates STAT-3 expression at the posttranscriptional level ( 9 ). In the present study, miR-223 and STAT-3 expression levels were altered to validate whether the RORα-miR-223/STAT-3 signalling pathway was involved in the regulation of melatonin in ECs exposed to low shear stress.…”

Section: Resultsmentioning

confidence: 99%

“…RORα regulates the expression of numerous genes at the transcriptional level and participates in a number of biological processes, including anti-inflammatory and anti-apoptotic processes ( 8 ). A previous study also revealed that microRNA (miRNA/miR)-223 inhibits signal transducer and activator of transcription 3 (STAT-3) signalling pathway activation and inhibits vascular calcification of smooth muscle cells ( 9 ). In addition, via bioinformatical analysis, the present study revealed that melatonin potentially binds to the miR-223 promoter and promotes miR-223 transcription.…”

Section: Introductionmentioning

confidence: 99%

Melatonin attenuates low shear stress‑induced pyroptosis and endothelial cell dysfunction via the RORα/miR‑223/STAT‑3 signalling pathway

Sui

Yang

2021

Exp Ther Med

View full text Add to dashboard Cite

Endothelial cells sense changes in blood flow shear stress and affect the progression of atherosclerotic plaques. Pyroptosis is an inflammatory form of cell death and has been implicated in cardiovascular diseases. Melatonin and its nuclear receptor retinoid-related orphan receptor α (RORα) have protective effects on the development of atherosclerosis. To date, whether melatonin can prevent endothelial cell pyroptosis and dysfunction in pathological shear stress remains unclear. In the present study, human umbilical vein endothelial cells (ECs) were cultured under low shear stress conditions (5 dyne/cm 2 ) for 24 h and treated with or without melatonin (2 µmol/l). The binding sites of the microRNA (miR)-223 promoter and RORα were predicted using the JASPAR website. Expression of pyroptosis-related proteins, including cleaved N-terminal gasdermin D, caspase-1, intercellular adhesion molecule 1 (ICAM-1) and nitric oxide (NO) were assessed. The results indicated that low shear stress increased pyroptosis and ICAM-1 expression, whereas it decreased NO levels. Melatonin alleviated pyroptosis and ICAM-1 expression and increased the production of NO in ECs. Further assessment revealed that low-level shear stress decreased RORα protein and mRNA expression, whereas melatonin would bind to RORα and thereby promoted miR-223 transcription in ECs. The present study also identified signal transducer and activator of transcription 3 (STAT-3) as a potential target gene of miR-223-3p. When transfected with miR-223 inhibitor, ECs up-regulated the expression of pyroptosis-related proteins and ICAM-1, and down-regulated NO levels. By contrast, silencing STAT-3 expression diminished the protective effect of miR-223. These results indicated that melatonin prevented ECs from undergoing pyroptosis and alleviated dysfunction via the RORα/miR-223/STAT-3 signalling pathway. This information could aid in the development of novel therapeutic approaches and provide new insights into atherosclerosis.

show abstract

“…Total RNAs were extracted from aortic valves and cells using TRIzol Reagent (Life technologies) as described previously (Han et al, 2021). RNA from five calcified aortic valves and 3 normal aortic valves were used for miRNA-sequencing.…”

Section: Rna Extraction and Mirna-sequencingmentioning

confidence: 99%

miRNA Expression Profiling Uncovers a Role of miR-139-5p in Regulating the Calcification of Human Aortic Valve Interstitial Cells

et al. 2021

Self Cite

View full text Add to dashboard Cite

Calcific aortic valve disease (CAVD) is the most common structural heart disease, and the morbidity is increased with elderly population. Several microRNAs (miRNAs) have been identified to play crucial roles in CAVD, and numerous miRNAs are still waiting to be explored. In this study, the miRNA expression signature in CAVD was analyzed unbiasedly by miRNA-sequencing, and we found that, compared with the normal control valves, 152 miRNAs were upregulated and 186 miRNAs were downregulated in calcified aortic valves. The functions of these differentially expressed miRNAs were associated with cell differentiation, apoptosis, adhesion and immune response processes. Among downregulated miRNAs, the expression level of miR-139-5p was negatively correlated with the osteogenic gene RUNX2, and miR-139-5p was also downregulated during the osteogenic differentiation of primary human aortic valve interstitial cells (VICs). Subsequent functional studies revealed that miR-139-5p overexpression inhibited the osteogenic differentiation of VICs by negatively modulating the expression of pro-osteogenic gene FZD4 and CTNNB1. In conclusion, these results suggest that miR-139-5p plays an important role in osteogenic differentiation of VICs via the Wnt/β-Catenin pathway, which may further provide a new therapeutic target for CAVD.

show abstract

MicroRNA-223-3p inhibits vascular calcification and the osteogenic switch of vascular smooth muscle cells

Cited by 36 publications

References 41 publications

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

Melatonin attenuates low shear stress‑induced pyroptosis and endothelial cell dysfunction via the RORα/miR‑223/STAT‑3 signalling pathway

miRNA Expression Profiling Uncovers a Role of miR-139-5p in Regulating the Calcification of Human Aortic Valve Interstitial Cells

Contact Info

Product

Resources

About