MicroRNA-221 regulates proliferation of bovine mammary gland epithelial cells by targeting the STAT5a and IRS1 genes

Jiao, Beilei; Zhang, X.L.; Wang, S.H.; Wang, L.X.; Luo, Zhixin; Zhao, Han; Khatib, Hasan; Wang, X.

doi:10.3168/jds.2018-15108

Cited by 29 publications

(23 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, our results indicate that miR-143 might promote milk fat synthesis by affecting the expression of the key regulators of lipid accumulation in BMECs. miRNAs can regulate multiple target genes, and exert their biological functions by different target genes in different cell types or in different cell states of the same cell type [30,31]. Chen et al [32] and Kim et al [33] revealed that miR-143 promotes adipogenesis by interacting with MAP2K5 and Pre-f-1 in adipocytes.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells

Zhang

Wang

et al. 2020

Animals

View full text Add to dashboard Cite

Milk fat is the main nutritional component of milk and is also an important indicator for evaluating milk quality. Substantial evidence has implicated miRNAs in the synthesis of milk fat. miR-143 is one of the miRNAs closely related to lipid metabolism. Herein, miR-143 upregulation remarkably promoted the production of lipid droplets and increased the level of intracellular triglyceride (TAG). Meanwhile, miR-143 suppression overtly repressed TAG synthesis and lipid droplet accumulation in bovine mammary epithelial cells (BMECs). At the same time, miR-143 significantly upregulated the genes associated with lipid synthesis, including PPARγ, SCD1, CEBPβ, and SREBP1. To examine the regulatory mechanism of miR-143 in milk fat synthesis, Smad3 was predicted as a new potential miR-143 target gene by TargetScan. Further studies found that miR-143 expression was inversely related to the levels of Smad3 mRNA and protein. Furthermore, luciferase reporter assays confirmed Smad3 to be a miR-143 direct target. Moreover, Smad3 gene silencing significantly increased intracellular TAG level in BMECs. These findings revealed that miR-143 promotes the TAG synthesis in BMECs via increasing the lipid synthesis related gens expression by targeting Smad3. The results of this study can be exploited in devising novel approaches for improving the nutritional value of milk in dairy cows.

show abstract

Section: Discussionmentioning

confidence: 99%

“…miRNAs can regulate multiple target genes, and exert their biological functions by different target genes in different cell types or in different cell states of the same cell type [ 30 , 31 ]. Chen et al [ 32 ] and Kim et al [ 33 ] revealed that miR-143 promotes adipogenesis by interacting with MAP2K5 and Pre-f-1 in adipocytes.…”

Section: Discussionmentioning

confidence: 99%

MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells

Zhang

Wang

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…Transfection of miR-221, which is highly expressed at peak compared with early lactation [101], into cultured bovine mammary gland epithelial cells inhibits cell proliferation and reduces the viability of these cells [155]. Dual luciferase assays have revealed that Stat5a, Stat3, and Insulin receptor substrate 1 (Irs1), key genes in the PI3KAkt/mTOR and JAK-STAT signalling pathways, bind directly to miR-221.…”

Section: Roles Of Mirnas In Mammary Gland Developmentmentioning

confidence: 99%

Epigenetics: New Insights into Mammary Gland Biology

Ivanova

Guillou

Hue-Beauvais

et al. 2021

Genes

View full text Add to dashboard Cite

The mammary gland undergoes important anatomical and physiological changes from embryogenesis through puberty, pregnancy, lactation and involution. These steps are under the control of a complex network of molecular factors, in which epigenetic mechanisms play a role that is increasingly well described. Recently, studies investigating epigenetic modifications and their impacts on gene expression in the mammary gland have been performed at different physiological stages and in different mammary cell types. This has led to the establishment of a role for epigenetic marks in milk component biosynthesis. This review aims to summarize the available knowledge regarding the involvement of the four main molecular mechanisms in epigenetics: DNA methylation, histone modifications, polycomb protein activity and non-coding RNA functions.

show abstract

“…The bovine mammary epithelial cell line was used to explore the function and regulatory mechanism of lncRNA and miRNA. It was obtained from the primary bovine mammary alveolar cells by stable transfection with SV-40 T antigen [33] and cultured in a 1:1 mixture of Ham's F-12 and Dulbecco's modi ed eagle medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% penicillin-streptomycin according to Jiao et al [34]. LncRNA MPNCR overexpression plasmid vector, miR-31 mimic or inhibitor were transfected using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions when the cells were at 60-80% con uence.…”

Section: Cell Culture and Transfectionmentioning

confidence: 99%

Long Non-Coding RNA MPNCR is a Negative Regulator of Bovine Mammary Epithelial Cell Proliferation

Wang¹,

Zhang²,

Liu³

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundNumerous studies have reported that many long non-coding RNAs (lncRNAs) may play multiple essential roles during mammary gland development; however, little is known about their functions and regulatory mechanism.ResultsIn this study, we confirmed a novel lncRNA, MPNCR, that was highly expressed during lactation compared to the dry period in dairy cattle. The potential biological and regulatory functions of MPNCR were explored in bovine mammary epithelial cells (BMECs). The MTT and EdU assays revealed that lncRNA MPNCR significantly suppressed cell viability and proliferation of BMECs. The subcellular localization indicated that MPNCR had a potential function as a competing endogenous RNA (ceRNA) to regulate the proliferation of BMECs. Bioinformatics analysis showed that lncRNA MPNCR had two binding sites for miR-31, further confirmed by the dual-luciferase report assay. The RT-qPCR result showed that the expression of miR-31 was downregulated following the overexpression of MPNCR in BMECs. Furthermore, the expression of MPNCR was significantly inhibited by miR-31 overexpression and upregulated by miR-31 inhibition. Cell viability and proliferation were significantly suppressed or promoted with the transfection of miR-31 inhibitor or miR-31 mimic into BMECs, respectively, whereas rescue with MPNCR reduced the proliferation of BMECs. The dual-luciferase report assay showed an interaction between miR-31 and the 3' UTR of the target gene CAMK2D. RT-qPCR and western blot further showed that the expression of CAMK2D was significantly increased with the overexpression of miR-31, while its expression was significantly suppressed with the inhibition of miR-31, indicating CAMK2D was a target of miR-31.ConclusionTaken together, these results demonstrate the function and regulatory mechanism of lncRNA MPNCR on the proliferation of BMECs, and that its regulation may contribute to bovine mammary cell population management.

show abstract

MicroRNA-221 regulates proliferation of bovine mammary gland epithelial cells by targeting the STAT5a and IRS1 genes

Cited by 29 publications

References 61 publications

MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells

MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells

Epigenetics: New Insights into Mammary Gland Biology

Long Non-Coding RNA MPNCR is a Negative Regulator of Bovine Mammary Epithelial Cell Proliferation

Contact Info

Product

Resources

About