MicroRNA‑218 inhibits the migration, epithelial‑mesenchymal transition and cancer stem cell properties of prostate cancer cells

Abstract

MicroRNA (miRNA) is a class of non-coding single-stranded RNA, able to regulate tumor-associated genes via binding the 3'-UTR of the target gene mRNA. Previous publications have demonstrated that miRNA-218 (miR-218) acts as a tumor-suppressive miRNA in various types of human cancer, including prostate cancer (PCa). However, the role of miR-218 in regulating PCa cell stemness and epithelial-mesenchymal transition remains unknown and requires further research. In the present study, it is demonstrated that miR-218 was downregulated in 2 PCa cell lines and could suppress cell migration, EMT and the exhibition of cancer stem cell-like properties. The expression of GLI family zinc finger 1 (Gli1) was inhibited by miR-218 overexpression, suggesting miR-218-suppression of Gli1 as a potential mechanism for the tumor-suppressive effect of miR-218. Overall, the results indicate that miR-218 served a critical role in the inhibition of PCa development. This may provide new insight for elucidating the mechanisms of PCa oncogenesis and suggests that miR-218 may be a novel therapeutic target for PCa.

RETRACTED: Downregulation of miR‐218 by nicotine promotes cell proliferation through targeting CDK6 in non–small cell lung cancer

“…17 Our findings also indicated that nicotine at concentration of 10 μM 15 could inhibit the expression of miR-218 in NSCLC cell lines. miR-218 plays an important role in cancer development, such as prostate cancer, 18 oral squamous cell carcinoma, 19 colorectal cancer, 20 ovarian cancer. 21 Except for osteoblasts, 22 miR-218 seems to be a tumor suppressor in various types of cancers.…”

LncRNA SOX2‐OT regulates AKT/ERK and SOX2/GLI‐1 expression, hinders therapy, and worsens clinical prognosis in malignant lung diseases

“…The MTA3/SOX2-OT/SOX2 axis has been involved as a prognostic factor for poor clinical outcomes in esophageal squamous cell carcinoma patients [40]. Results from our study confirm that overexpressed LncRNA SOX2-OT is involved in the control of SOX2-OT/SOX2/GLI-1 axis expression, as probable key archetypes, including as "scaffold" to RNA-binding protein (RBP) [65], and ceRNA function reported on SOX2-targeting miR200c [15], or hypothetically involved in ceRNA activity on miR326 [66], or miR218 [67] whose miRNAs are involved in GLI-1targeting. Some of these functional archetypes have been shown or suggested to participate in therapy resistance and clinical prognosis in lung cancer, as it has been previously reported [68].…”

<p>α-E-Catenin (CTNNA1) Inhibits Cell Proliferation, Invasion and EMT of Bladder Cancer</p>

“…Previous studies demonstrated that EMT was an important progress in tumor development and metastasis. 39 Guan et al 40 found that microRNA-218 suppressed EMT and migration in prostate cancer. Chockley et al 41 reported that in lung cancer, EMT could cause metastasis-specific immunosurveillance that mediated by natural killer cells.…”