microRNA‐216b inhibits cell proliferation and migration in human melanoma by targeting FOXM1 in vitro and in vivo

Sun, Mengyao; Wang, Xiaopeng; Tu, Chen‐Pei D.; Wang, Shuang; Qu, Jianqiang; Xiao, Shengxiang

doi:10.1002/cbin.10754

Cited by 24 publications

(13 citation statements)

References 25 publications

(30 reference statements)

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“…In this same study, the authors identify forkhead box protein M1 (FOXM1) as a direct target, and ectopic expression of miR-216b led to a decrease in FOXM1 protein levels and percentage of Ki-67-positive cells in xenograft models. A similar mechanism has been shown for melanoma (99), hepatocellular carcinoma (100), non-small-cell lung carcinoma (101), and osteosarcoma (102), and low miR-216b levels in tumors are independent poor prognostic indicators in many of these cancers. In pancreatic cancer, this miRNA functions in a tumor-suppressive way by targeting translationally controlled tumor protein (TPT1), and levels of this miRNA are significantly associated with large tumor size and advanced TNM stage (103).…”

Section: Mir-494: Upregulated In Hypoxia In Canine High-grade Glioma supporting

confidence: 62%

Differential Expression of miRNAs in Hypoxia (“HypoxamiRs”) in Three Canine High-Grade Glioma Cell Lines

et al. 2020

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Dogs with spontaneous high-grade gliomas increasingly are being proposed as useful large animal pre-clinical models for the human disease. Hypoxia is a critical microenvironmental condition that is common in both canine and human high-grade gliomas and drives increased angiogenesis, chemo-and radioresistance, and acquisition of a stem-like phenotype. Some of this effect is mediated by the hypoxia-induced expression of microRNAs, small (∼22 nucleotides long), non-coding RNAs that can modulate gene expression through interference with mRNA translation. Using an in vitro model with three canine high-grade glioma cell lines (J3T, SDT3G, and G06A) exposed to 72 h of 1.5% oxygen vs. standard 20% oxygen, we examined the global "hypoxamiR" profile using small RNA-Seq and performed pathway analysis for targeted genes using both Panther and NetworkAnalyst. Important pathways include many that are well-established as being important in glioma biology, general cancer biology, hypoxia, angiogenesis, immunology, and stem-ness, among others. This work provides the first examination of the effect of hypoxia on miRNA expression in the context of canine glioma, and highlights important similarities with the human disease.

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Section: Mir-494: Upregulated In Hypoxia In Canine High-grade Glioma supporting

confidence: 62%

Differential Expression of miRNAs in Hypoxia (“HypoxamiRs”) in Three Canine High-Grade Glioma Cell Lines

et al. 2020

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“…16 The cells in the 24-well culture plates were transfected with FoxM1-WT or FoxM1-MUT and miR-216b mimics (20 nmol/L) by Lipofectamine™ 2000. The MUT sequence of FoxM1 has been confirmed in a previous study.…”

Section: Luciferase Assaymentioning

confidence: 99%

MiR‐216b inhibits osteosarcoma cell proliferation, migration, and invasion by targeting Forkhead Box M1

Wang

Guo

et al. 2018

J of Cellular Biochemistry

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Osteosarcoma (OS) is considered the most common type of primary malignant bone tumor, which has a high rate of mortality in children and adolescents. However, the current treatment methods for OS are ineffective. Therefore, there is an urgent requirement to identify the critical targets. This study aimed to identify the roles and significance of microRNA‐216b (miR‐216b) in OS. To explore the cellular and molecular functions of miR‐216b and Forkhead Box M1 (FoxM1) in OS, the expression of miR‐216b and FoxM1 at the transcriptional level was measured using quantitative real‐time PCR (qRT‐PCR). Wound healing assay, 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyltetrazolium bromide assay (MTT) assay, flow cytometry, and transwell invasion assay were conducted to study the function of miR‐216b and FoxM1 in OS cells. Dual luciferase reporter assay was performed to identify the relationships between miR‐216b and FoxM1. qRT‐PCR results revealed that miR‐216b expression was significantly downregulated, and FoxM1 was observed to be significantly upregulated in human OS cell lines (MG‐63) and tissues. MTT data showed that upregulation of miR‐216b expression led to cell growth inhibition in MG‐63 cells. The results of the invasion assay and wound healing assay illustrated that miR‐216b upregulation or FoxM1 downregulation could inhibit the invasion and migration in MG‐63 cells. In vivo, the tumor volume was significantly decreased by miR‐194 mimic treatment compared with the control group. Furthermore, the results of the luciferase assay indicated that FoxM1 is a direct target of miR‐216b. These findings may provide novel insights into the molecular mechanism of miR‐216b and FoxM1 in the progression of OS, and suggested that miR‐216b may serve as a potential tumor inhibitor of OS by targeting FoxM1.

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“…Numerous studies have indicated that miRNAs play pivotal roles in several biological processes, including cell proliferation, cycle, apoptosis, differentiation, migration and metastasis (13)(14)(15). Recently, dysregulation of miRNA expression has been observed in various types of human cancers, such as melanoma (16), gastric (17), lung (18) and colorectal cancer (19) and glioma (20). In the context of cancer, miRNAs may serve as oncogenes or tumour suppressors in tumourigenesis and tumour development by negatively regulating tumour suppressors or oncogenes (21,22).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-326 inhibits melanoma progression by targeting KRAS and suppressing the AKT and ERK signalling pathways

Kang

Zhang

et al. 2017

Oncol Rep

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Abstract. Melanoma is the seventh most common malignancy in females and the fifth most common cancer in males worldwide. An increasing number of studies have reported that microRNA (miRNA) dysregulation is frequently observed in various types of human cancers, including melanoma. Abnormally expressed miRNAs play an important role in melanoma formation and progression by serving as potential biomarkers and therapeutic targets. Recently, miRNA-326 (miR-326) has been reported to be differentially expressed in various types of tissues and play important roles in tumourigenesis and tumour development. However, the expression levels, biological roles and underlying mechanisms of miR-326 in melanoma remain unknown. In the present study, we demonstrated that miR-326 was significantly downregulated in melanoma tissues and cell lines. Functional assays revealed that the enforced expression of miR-326 suppressed melanoma cell proliferation and invasion and increased cell apoptosis in vitro. Using bioinformatic analysis, Kirsten rat sarcoma viral oncogene homolog (KRAS) was predicted as a potential target of miR-326. Luciferase reporter assay confirmed that miR-326 could directly target the 3'-untranslated region of KRAS. In addition, reverse transcription-quantitative polymerase chain reaction and western blot analysis revealed that miR-326 upregulation decreased the KRAS expression in melanoma cells at both the mRNA and protein level. Furthermore, KRAS was upregulated in melanoma tissues and inversely correlated with miR-326 expression. In addition, the KRAS knockdown phenocopied the tumour-suppressing effects of miR-326 overexpression on melanoma cells. The restoration of the expression of KRAS markedly reversed the antitumour effects induced by miR-326 overexpression in melanoma cells. Further experiments indicated that miR-326 inactivated the AKT and ERK signalling pathways in melanoma. Collectively, these results revealed that miR-326 serves as a tumour suppressor in melanoma by targeting KRAS and regulating the AKT and ERK signalling pathways, indicating that miR-326 may be a promising therapeutic target for melanoma patients.

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microRNA‐216b inhibits cell proliferation and migration in human melanoma by targeting FOXM1 in vitro and in vivo

Cited by 24 publications

References 25 publications

Differential Expression of miRNAs in Hypoxia (“HypoxamiRs”) in Three Canine High-Grade Glioma Cell Lines

Differential Expression of miRNAs in Hypoxia (“HypoxamiRs”) in Three Canine High-Grade Glioma Cell Lines

MiR‐216b inhibits osteosarcoma cell proliferation, migration, and invasion by targeting Forkhead Box M1

MicroRNA-326 inhibits melanoma progression by targeting KRAS and suppressing the AKT and ERK signalling pathways

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